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台湾省苹果上牛眼果腐病菌的截获鉴定
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引用本文：张慧丽,李雪莲,段丽君,徐瑛,陈先锋,段维军.台湾省苹果上牛眼果腐病菌的截获鉴定.植物保护学报,2019,46(1):90-96
DOI：10.13802/j.cnki.zwbhxb.2019.2019911
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作者单位E-mail
张慧丽 宁波检验检疫科学技术研究院, 浙江 宁波 315012  
李雪莲 宁波检验检疫科学技术研究院, 浙江 宁波 315012  
段丽君 宁波检验检疫科学技术研究院, 浙江 宁波 315012  
徐瑛 宁波检验检疫科学技术研究院, 浙江 宁波 315012  
陈先锋 宁波检验检疫科学技术研究院, 浙江 宁波 315012  
段维军 宁波检验检疫科学技术研究院, 浙江 宁波 315012 weijunduan@tom.com 
中文摘要:为明确从宁波空港口岸入境旅客携带苹果上截获的1株明孢盘菌属菌株60017的分类地位，通过形态学特征观察、内转录间隔区（ITS）和β-微管蛋白基因（β-tubulin）序列比对分析以及致病性测定，对该菌株进行了种类鉴定。结果表明：菌株60017在PDA培养基上的菌落为圆形，边缘整齐，呈乳白色，气生菌丝较少，可产生大量分生孢子；基于ITS和β-tubulin序列构建的系统发育树中，菌株60017和苹果牛眼果腐病菌Neofabraea kienholzii处于同一分支；菌株60017接种苹果后，在接种部位能引起褐腐症状，与原发病症状一致。结合形态学特征、致病性测定结果以及分子鉴定结果，最终将该菌株鉴定为苹果牛眼果腐病菌N.kienholzii。
中文关键词:苹果牛眼果腐病菌  形态观察  序列分析  致病性测定  检测鉴定
 
Isolation and identification of Neofabraea kienholzii on Malus pumila imported from Taiwan Province
Author NameAffiliationE-mail
Zhang Huili Ningbo Academy of Inspection and Quarantine, Ningbo 315012, Zhejiang Province, China  
Li Xuelian Ningbo Academy of Inspection and Quarantine, Ningbo 315012, Zhejiang Province, China  
Duan Lijun Ningbo Academy of Inspection and Quarantine, Ningbo 315012, Zhejiang Province, China  
Xu Ying Ningbo Academy of Inspection and Quarantine, Ningbo 315012, Zhejiang Province, China  
Chen Xianfeng Ningbo Academy of Inspection and Quarantine, Ningbo 315012, Zhejiang Province, China  
Duan Weijun Ningbo Academy of Inspection and Quarantine, Ningbo 315012, Zhejiang Province, China weijunduan@tom.com 
Abstract:To identify the fungal strain 60017 isolated from the apple imported from Taiwan Province of China, the morphological observation, sequence analysis and pathogenicity test were carried out. The morphological results showed that the colony of the strain 60017 on PDA medium was round, with neat edges, milky white, less aerial hyphae, and could produce a large number of conidia. Its rDNA ITS and β-tubulin sequences were sequenced. Analysis of these sequences with the related sequences in NCBI showed that the strain 60017 clustered into Neofabraea kienholzii clade. The pathogenicity test showed that the strain 60017 could infect apple fruit with the symptoms of brown rot. These results indicated that the fungal strain 60017 was N. kienholzii.
keywords:Neofabraea kienholzii  morphological characteristics  sequence analysis  pathogenicity  detection
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