| 棉铃疫病生防细菌筛选、鉴定及制剂防治效果 |
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| 引用本文：鹿秀云,李宝庆,张晓云,郭庆港,马平,李社增.棉铃疫病生防细菌筛选、鉴定及制剂防治效果.植物保护学报,2019,46(4):805-815 |
| DOI：10.13802/j.cnki.zwbhxb.2019.2018110 |
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| 作者 | 单位 | E-mail | | 鹿秀云 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | | | 李宝庆 | 临沂市农业技术推广服务中心, 山东 临沂 276000 | | | 张晓云 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | | | 郭庆港 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | | | 马平 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | | | 李社增 | 河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000 | shezengli@163.com |
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| 中文摘要:为有效防治由苎麻疫霉Phytophthora boehmeriae引起的棉铃疫病，采用平板对峙培养法、离体试验和田间小区试验对棉花、玉米、番茄、黄瓜等作物根围细菌进行筛选，结合形态学观察、16SrDNA序列分析及Biolog微生物自动鉴定系统对筛选出的生防细菌进行分类鉴定，并通过离体试验对优秀生防菌2种制剂的防治效果进行评价。拮抗细菌初筛试验结果表明，在供试的1 250株作物根围细菌中，399株细菌菌株有良好的抑菌能力，其中40株拮抗细菌菌株抑菌率在80.00%以上，菌株HMB22922抑菌能力突出，对棉铃疫病菌的抑菌率为85.71%，抑菌带宽10.0 mm。室内筛选试验结果表明，菌株HMB22922、HMB23917和HMB21405的病情指数显著低于空白对照，与化学药剂对照间差异不显著，人工接种苎麻疫霉5 d和7 d后，3株菌株对棉铃疫病的防治效果分别为73.92%、69.58%、56.54%和81.34%、66.67%、64.60%。田间筛选试验结果表明，菌株HMB22922培养液对棉铃疫病的田间防治效果为47.12%~58.64%，优于其它2株供试菌株，与化学药剂的防治效果相当且防治效果稳定。生防菌株HMB22922经形态学观察、序列分析和和微生物鉴定系统被鉴定为萎缩芽胞杆菌Bacillus atrophaeus。人工接种苎麻疫霉5、7和12 d时，以菌株HMB22922为有效成分的2.5×109 CFU/mL萎缩芽胞杆菌悬浮剂的防治效果分别为86.33%、78.66%和64.44%，均优于其相同菌量培养液的防治效果。 |
| 中文关键词:棉铃疫病 生防细菌 苎麻疫霉 棉花 微生物杀菌剂 |
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| Screening, identification and evaluation of biocontrol bacteria against cotton boll blight |
| Author Name | Affiliation | E-mail | | Lu Xiuyun | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture
IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | | | Li Baoqing | Linyi Agricultural Technology Promotion Center, Linyi 276000, Shandong Province, China | | | Zhang Xiaoyun | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture
IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | | | Guo Qinggang | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture
IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | | | Ma Ping | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture
IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | | | Li Shezeng | Key Laboratory of Integrated Pest Management on Crops in Northern Region of North China, Ministry of Agriculture
IPM Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China | shezengli@163.com |
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| Abstract:To effectively prevent cotton boll blight caused by Phytophthora boehmeriae, the bacterial strains isolated from rhizospheres of crops such as cotton, corn, tomato, cucumber, etc. were screened and evaluated with dual-culture assay, in vitro test and field trial. Identification of the potential biocontrol strain was based on morphological characters, 16S rDNA sequences and microbial identification system. The preparations of potential biocontrol bacteria against cotton boll blight were evaluated with in vitro test for their control efficacy. A total of 399 antagonistic bacteria with strong inhibitory abilities against P. boehmeriae were obtained from 1 250 tested bacterial strains, and 40 bacteria showed more than 80.00% inhibitory rate. Among them, strain HMB22922 showed the highest antagonistic activities with an inhibition rate of 85.71% and an inhibition zone of 10.0 mm. Strains HMB22922, HMB23917 and HMB21405 showed lower disease indices than the negative control, and no significant difference was observed compared to the chemical fungicide. The control efficacy against cotton boll blight of the strain HMB22922, HMB23917 and HMB21405 were 73.92%, 69.58% and 56.54% at the 5th day after inoculation and 81.34%, 66.67% and 64.60% at the 7th day after inoculation, respectively. Field trials showed that the biocontrol efficacy of strain HMB22922 was stable and similar to chemical fungicide, with a biocontrol efficacy of 47.12%-58.64% against cotton boll blight. Strain HMB22922 was identified as Bacillus atrophaeus based on morphological characters, 16S rDNA and microbial identification system. The preparation of 2.5×109 CFU/mL B. atrophaeus SC, developed from HMB22922, showed higher biocontrol efficacy than the bacterium culture broth, and 86.33%, 78.66% and 64.44% biocontrol efficacies were obtained at the 5th, 7th and 12th day after inoculation, respectively. |
| keywords:cotton boll blight biocontrol bacteria Phytophthora boehmeriae cotton microbial fungicide |
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