| 糜子细菌性条斑病病原菌鉴定及其对13种杀菌剂的敏感性 |
| 点此下载全文 |
| 引用本文：白雪,陈悦,白庆荣,谷月,孙蕾,赵廷昌.糜子细菌性条斑病病原菌鉴定及其对13种杀菌剂的敏感性.植物保护学报,2019,46(6):1233-1242 |
| DOI：10.13802/j.cnki.zwbhxb.2019.2018144 |
| 摘要点击次数: |
| 全文下载次数: |
|
|
| 中文摘要:为有效防治糜子细菌性条斑病，采用平板划线分离法对2016年自黑龙江省齐齐哈尔市采集的罹细菌性条斑病的糜子植株进行病原菌分离与纯化，依据柯赫氏法则对其致病性进行测定；采用Biolog自动微生物鉴定系统、16S rDNA和16S-23S rDNA间隔区序列分析及病原菌的生理生化反应对病原菌进行鉴定；并测定该病原菌对13种常用杀菌剂的敏感性。结果显示，从糜子叶片和叶鞘病斑中共分离纯化获得10株培养性状一致的菌株，经柯赫氏法则验证均为细菌性条斑病病原菌。经Biolog鉴定，3株代表菌株与标准菌株燕麦噬酸菌Acidovorax avenae的相似值分别为0.716、0.705、0.723，序列分析显示，这3株代表菌株与燕麦噬酸菌燕麦亚种A.avenae subsp.avenae的一致性为99%以上；该病原菌的革兰氏染色反应及碳源利用等生理生化反应与对照菌株燕麦噬酸菌ICMP3183表现一致。因此，确定糜子细菌性条斑病病原菌为燕麦噬酸菌。糜子细菌性条斑病病原菌代表菌株MZ121101对80%乙蒜素乳油、30%乙蒜素乳油及0.3%四霉素水剂的敏感性较高，抑制中浓度EC50介于389.31~939.94 μg/mL之间，这2种药剂可作为防治糜子细菌性条斑病的首选药剂。 |
| 中文关键词:糜子 细菌性条斑病 燕麦噬酸菌 病原菌鉴定 杀菌剂 敏感性 |
| |
| Identification of pathogen causing bacterial stripe of millet and its susceptibility to 13 bactericides |
| Author Name | Affiliation | E-mail | | Bai Xue | College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China
Institute of Plant Protection, Jilin Academy of Agricultural Sciences, Gongzhuling 136100, Jilin Province, China | | | Chen Yue | College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China | | | Bai Qingrong | College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China | bbbqqqrrr@163.com | | Gu Yue | College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China | | | Sun Lei | College of Plant Protection, Jilin Agricultural University, Changchun 130118, Jilin Province, China | | | Zhao Tingchang | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | zhaotgcg@163.com |
|
|
| Abstract:To control millet bacterial stripe, the method of streaking plate was used to isolate pathogen from the diseased samples that were collected from Qiqihaer City, Heilongjiang Province in 2016. The pathogenicity of the isolates was tested to fulfill by the Koch's postulates. Biolog systematic, sequences analyses of 16S rDNA and 16S-23S rDNA, biochemical and physiological reactions were used in pathogen identification. The sensitivity of representative strain to 13 bactericides was also determined. Ten isolates were obtained from the diseased leaves and sheaths with similar morphology on medium, and were verified as the pathogens of the bacterial stripe of millet. The results of Biolog test showed three representative strains were similar with Acidovorax avenae with similarity values of 0.716, 0.705 and 0.723, respectively. Sequences analysis showed that the three representative strains were more than 99% consistent with A.avenae subsp. avenae. Physiological and biochemical reactions such as Gram staining and carbon utilization of these isolates were consistent with those of control strain A.avenae ICMP3183, thus the pathogen was eventually identified as Acidovorax avenae. The MZ121101 strain was sensitive to 80% ethylicin EC, 30% ethylicin EC and 0.3% tetramycin AS, the EC50 of them was 389.31-939.94 μg/mL。The two fungicides could be used to control bacterial stripe of millet in the field. |
| keywords:millet bacterial stripe Acidovorax avenae pathogen identification bactericide susceptibility |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 8715799 版权所有：植物保护学报 京ICP备05006550号-2 主管单位：中国科协 主办单位：中国植物保护学会、中国农业大学 地址：北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话：010-62732528 电子邮件：zbxb@cau.edu.cn 技术支持： 北京勤云科技发展有限公司 |
