| 辽宁省稻曲病菌遗传多样性和致病力分析 |
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| 引用本文：李昕洋,魏松红,桑海旭,王井士,张照茹,王应玲.辽宁省稻曲病菌遗传多样性和致病力分析.植物保护学报,2020,47(1):84-92 |
| DOI：10.13802/j.cnki.zwbhxb.2020.2019075 |
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| 中文摘要:为有效防治辽宁省稻曲病菌Ustilaginoidea virens，利用重复序列PCR（repetitive elementbased PCR，rep-PCR）分子指纹技术，对2017年自辽宁省8个市8个主产稻区采集的51株稻曲病菌菌株进行遗传多样性和致病力分析。结果显示，在3对引物中，以BOX1/BOX2和ERIC1/ERIC2为引物扩增的DNA指纹图谱的遗传多样性值分别为0.764、0.707，均大于0.7，故选择这2种引物扩增的DNA指纹图谱进行遗传多样性分析；当DNA指纹相似系数为0.78时，以BOX1/BOX2为引物和以ERIC1/ERIC2为引物扩增的DNA指纹图谱分别将供试菌株划分为12个和10个遗传类群；供试菌株致病力可划分为弱致病型、中等致病型和强致病型3个致病型，所占比例分别为33.33%、58.82%和7.85%，强致病型菌株仅在沈阳市、鞍山市和大连市出现；所有优势类群均包含3种致病型菌株。表明辽宁省稻曲病菌遗传结构复杂，不同地理来源的稻曲病菌菌株致病力存在一定差异，相同致病型的稻曲病菌菌株分属于不同的遗传类群，同一遗传类群中包含不同的致病型菌株。 |
| 中文关键词:稻曲病菌 重复序列PCR 遗传多样性 致病力 聚类分析 |
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| Analysis of genetic diversity and pathogenicity of rice false smut pathogen Ustilaginoidea virens in Liaoning Province |
| Author Name | Affiliation | E-mail | | LI Xinyang | College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, Liaoning Province, China | | | WEI Songhong | College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, Liaoning Province, China | songhongw125@163.com | | SANG Haixu | Saline-alkali Land Utilization and Research Institute, Liaoning Academy of Agricultural Sciences, Panjin 124010, Liaoning Province, China | | | WANG Jingshi | Saline-alkali Land Utilization and Research Institute, Liaoning Academy of Agricultural Sciences, Panjin 124010, Liaoning Province, China | | | ZHANG Zhaoru | College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, Liaoning Province, China | | | WANG Yingling | College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, Liaoning Province, China | |
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| Abstract:In order to effectively control rice false smut pathogen Ustilaginoidea virens in Liaoning Province, a total of 51 strains of U. virens collected from eight main rice-producing areas in eight cities of Liaoning Province in 2017 were analyzed for their genetic diversity and pathogenicity by using the repetitive element-based PCR (rep-PCR) molecular fingerprinting techniques. The results showed that the genetic diversities of DNA fingerprints amplified with BOX1/BOX2 and ERIC1/ERIC2 primers were 0.764 and 0.707, respectively, which were greater than 0.7. The DNA fingerprints amplified by these two primers were thus selected for genetic diversity analysis. When the similarity coefficient of DNA fingerprints was 0.78, the amplified fingerprints using BOX1/BOX2 and ERIC1/ERIC2 as the primers divided the tested strains into 12 and ten genetic groups, respectively. The tested strains were divided into three pathogenicity types, among which weak pathogenicity type, moderate pathogenicity type and strong pathogenicity type accounted for 33.33%, 58.82% and 7.85%, respectively. The strong pathogenicity type only appeared in Shenyang, Anshan and Dalian cities. All dominant groups contained three pathogenicity types. The results indicated that the genetic structure of U. virens was complicated in Liaoning Province. There were some differences in pathogenicity of the strains of U. virens from different geographical sources. The same pathogenicity type of U. virens belonged to different genetic groups and the same genetic group contained different pathogenic types. |
| keywords:Ustilaginoidea virens repetitive element-based PCR genetic diversity pathogenicity cluster analysis |
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