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黑龙江省稻瘟病菌育性及其交配型分析

引用本文：张晓玉,张亚玲,靳学慧,周弋力,孟峰,武菁菁.黑龙江省稻瘟病菌育性及其交配型分析.植物保护学报,2020,47(1):93-100

DOI：10.13802/j.cnki.zwbhxb.2020.2019058

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作者	单位	E-mail
张晓玉	黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319
张亚玲	黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319
靳学慧	黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319	Jxh2686@163.com
周弋力	黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319
孟峰	黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319
武菁菁	黑龙江八一农垦大学, 黑龙江省植物抗性研究中心, 大庆 163319

中文摘要:为明确黑龙江省采集自不同年份、不同地区的稻瘟病菌Magnaporthe oryzae的育性能力和交配型分布，采用2株标准菌株GUY11（MAT1-2）和KA3（MAT1-1）对2016-2017年黑龙江省西部、东部、中部3个地区经单孢分离的241株稻瘟病菌进行育性测定，并利用PCR技术对其交配型进行检测。结果表明，黑龙江省西部、东部、中部的241株稻瘟病菌中可育性菌株比例为11.62%，其中雌性菌株、雄性菌株、两性菌株分别占1.66%、4.56%和1.25%，不能判断其性别的未知菌株占4.15%。采集自不同地区、不同年份的稻瘟病菌可育性差异均较大，西部、东部、中部地区可育性菌株出现频率分别为13.25%、7.27%和12.62%；2016年采集的稻瘟病菌可育性较高，可育性菌株出现频率为25.30%。黑龙江省稻瘟病菌群体中同时存在MAT1-1和MAT1-2两种交配型，主要以交配型MAT1-1占优势，出现频率为58.92%，交配型为MAT1-2的菌株出现频率为8.30%。不同地区稻瘟病菌的交配型亦有差异，交配型为MAT1-1的菌株在黑龙江省东部地区出现频率最高，为72.73%，在中部、西部地区的出现频率次之，分别为61.17%和46.99%。表明黑龙江省水稻种植区的稻瘟病菌同时存在2种交配型菌株，其交配型存在丰富的多态性，但其可育性及交配型分布不均衡。

中文关键词:稻瘟病菌有性生殖交配型育性 PCR

Analysis of the fertility and mating type of rice blast fungus Magnaporthe oryzae in Heilongjiang Province

Author Name	Affiliation	E-mail
ZHANG Xiaoyu	Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China
ZHANG Yaling	Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China
JIN Xuehui	Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China	Jxh2686@163.com
ZHOU Yili	Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China
MENG Feng	Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China
WU Jingjing	Research Center of Plant Resistance in Heilongjiang, Heilongjiang Bayi Agricultural University, Daqing 163319, Heilongjiang Province, China

Abstract:In order to clarify the distribution of fertility and mating types of Magnaporthe oryzae collected from different regions of Heilongjiang Province and in different years, 241 isolates of M. grisea collected from western, eastern and central regions of Heilongjiang Province from 2016 to 2017 were tested for their mating types and fertility with two standard test isolates GUY11 (MAT1-2) and KA3 (MAT1-1) and their mating types were detected by PCR. The results showed that the percentage of fertile isolates in 241 isolates of M. oryzae was 11.62%, of which female isolates, male isolates and amphipathic isolates accounted for 1.66%, 4.56% and 1.25%, respectively, and unknown isolates that could not judge their sex accounted for 4.15%. The fertility of rice blast fungus collected from different regions and in different years varied greatly. The frequency of fertility isolates in western, eastern and central regions was 13.25%, 7.27% and 12.62%, respectively. The frequency of fertility isolates of M. oryzae collected in 2016 was higher (25.30%). There were two mating types of MAT1-1 and MAT1-2 existing simultaneously in Heilongjiang Province:mating type MAT1-1 was dominant, the frequency was 58.92%, and the frequency of mating type MAT1-2 was 8.30%. The mating types of M. oryzae in different regions also differed greatly. The isolate with mating type MAT1-1 occurred the most frequently in the eastern part of Heilongjiang Province, the frequency was 72.73%, followed by the frequencies of the central and western regions, accounting for 61.17% and 46.99%, respectively. The results indicated that there were two mating isolates in the rice-growing areas of Heilongjiang Province, and the mating type was rich in polymorphism, but the distribution of fertility and mating type was imbalanced.

keywords:Magnaporthe oryzae sexual reproduction mating type fertility PCR

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