| 贝莱斯芽胞杆菌NSZ-YBGJ001的鉴定及其生物学特性 |
| 点此下载全文 |
| 引用本文：陶永梅,潘洪吉,张小利,黄健,李鹏,卢志军.贝莱斯芽胞杆菌NSZ-YBGJ001的鉴定及其生物学特性.植物保护学报,2020,47(2):324-331 |
| DOI：10.13802/j.cnki.zwbhxb.2020.2019115 |
| 摘要点击次数: |
| 全文下载次数: |
|
|
| 中文摘要:为明确分离自海南省三亚市陵水县土壤中的菌株NSZ-YBGJ001的分类地位和生物学特性，利用形态特征观察、生理生化特性测定、16S rRNA和gyrB基因序列比对及系统进化分析对其进行分类鉴定，测定并绘制其生长曲线，对该菌株在不同pH、NaCl浓度和温度下的生长繁殖进行研究，评价其抗逆性。结果表明：结合菌株NSZ-YBGJ001的形态特征、生理生化特性以及16S rRNA和gyrB基因序列分析，将该菌株鉴定为贝莱斯芽胞杆菌Bacillus velezensis。菌株NSZ-YBGJ001生长较快，繁殖量大，接种后4 h即进入对数生长期，16~24 h为稳定生长期，24 h时菌液浓度最高，达到1.89×109 CFU/mL，然后进入衰退期，一直到44 h菌液浓度维持在1.79×108 CFU/mL左右。该菌在pH 5~9、2%~10% NaCl和20~45℃条件下均可以正常生长；在pH低于4或高于10、NaCl浓度高于15%、温度超过50℃的条件下菌株NSZ-YBGJ001生长受到严重抑制，菌液浓度随生长条件极端化而显著降低；当pH超过13，NaCl浓度超过20%时菌株死亡。 |
| 中文关键词:贝莱斯芽胞杆菌 种类鉴定 生物学特性 抗逆性 |
| |
| Identification and biological characteristics of Bacillus velezensis NSZ-YBGJ001 |
| Author Name | Affiliation | E-mail | | TAO Yongmei | College of Plant Protection, China Agricultural University, Beijing 100193, China | | | PAN Hongji | Beijing Plant Protection Station, Beijing 100029, China | | | ZHANG Xiaoli | Beijing Plant Protection Station, Beijing 100029, China | | | HUANG Jian | Beijing Agricultural Products Quality Production and Marketing Service Station, Beijing 102220, China | | | LI Peng | Hainan Agricultural Department, Haikou 570204, Hainan Province, China | | | LU Zhijun | Beijing Plant Protection Station, Beijing 100029, China | lerrylu@126.com |
|
|
| Abstract:In order to clarify the taxonomic status and biological characteristics of strain NSZ-YBGJ001 isolated from the soil of Lingshui County, Sanya City, Hainan Province, the colony characteristics, morphological characteristics, physiological and biochemical characteristics, 16S rRNA and gyrB gene sequences and phylogenetics were analyzed. The growth curve of strain NSZ-YBGJ001 was determined and drawn, and the growth and reproduction of strain NSZ-YBGJ001 were studied under different pH values, NaCl concentrations and temperatures to evaluate its stress resistance. The results showed that the strain NSZ-YBGJ001 was identified as Bacillus velezensis according to its morphological characteristics, physiological and biochemical characteristics, 16S rRNA and gyrB gene sequence analysis. The strain NSZ-YBGJ001 grew rapidly and had a high reproductive capacity. It entered the logarithmic phase at 4 h after inoculation and the stable phase at 16-24 h; the highest concentration (1.89×109 CFU/mL) was observed at 24 h, followed by the decline phase, lasting until 44 hours when the concentration of bacterial solution maintained at about 1.79×108 CFU/mL. Under the conditions of pH 5-9, 2%-10% NaCl and 20-45℃, the strain could grow normally; under the conditions of pH lower than 4 or higher than 10, NaCl concentration higher than 15%, and temperature higher than 50℃, the growth of the strain was severely inhibited, and the concentration of bacterial solution significantly decreased under the extreme growth conditions; when pH exceeded 13 and the concentration of NaCl exceeded 20%, the strain died. |
| keywords:Bacillus velezensis species identification biological characteristics stress resistance |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 8710527 版权所有：植物保护学报 京ICP备05006550号-2 主管单位：中国科协 主办单位：中国植物保护学会、中国农业大学 地址：北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话：010-62732528 电子邮件：zbxb@cau.edu.cn 技术支持： 北京勤云科技发展有限公司 |
