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草地贪夜蛾取食诱导玉米叶片转录组分析

引用本文：黄欣蒸,单双,张丹丹,张天涛,石旺鹏,张永军.草地贪夜蛾取食诱导玉米叶片转录组分析.植物保护学报,2020,47(4):780-788

DOI：10.13802/j.cnki.zwbhxb.2020.2020814

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作者	单位	E-mail
黄欣蒸	中国农业大学植物保护学院昆虫学系, 农业农村部作物有害生物监测与绿色防控重点实验室, 北京 100193
单双	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
张丹丹	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
张天涛	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
石旺鹏	中国农业大学植物保护学院昆虫学系, 农业农村部作物有害生物监测与绿色防控重点实验室, 北京 100193
张永军	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193	yjzhang@ippcaas.cn

中文摘要:为阐明玉米响应草地贪夜蛾Spodoptera frugiperda取食胁迫防御反应的分子机制，利用RNA-seq技术对草地贪夜蛾取食的玉米叶片进行转录组测序分析，并对抗虫代谢产物生物合成途径中的基因进行筛选鉴定。结果显示，与未接虫对照相比，草地贪夜蛾取食18 h导致玉米叶片中有1 645个基因差异表达（log₂|处理/对照|>1且FDR<0.05），其中上调表达基因有1 352个，下调表达基因有293个。茉莉酸、水杨酸、乙烯等植物激素生物合成与信号转导途径相关基因大多上调表达，其中44个茉莉酸途径相关基因全部上调表达，说明该途径在玉米响应草地贪夜蛾取食诱导的防御反应中发挥着核心作用，其它激素生物合成与信号转导途径发挥协同作用。玉米重要抗虫次生代谢物苯并噁唑嗪酮类生物合成相关基因中有9个基因上调表达；15个萜烯挥发物生物合成相关差异表达基因中有14个上调表达，包括8个萜烯合成酶基因和1个CYP基因CYP92C5。表明草地贪夜蛾取食会诱导玉米复杂的植物激素途径和基因调控机制，激活以次级抗虫代谢物合成为主的防御反应。

中文关键词:化学通讯生态调控信号分子植物挥发物萜烯合成酶

Transcriptome analysis of Zea mays leaves infested by fall armyworm Spodoptera frugiperda larvae

Author Name	Affiliation	E-mail
HUANG Xinzheng	Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China
SHAN Shuang	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
ZHANG Dandan	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
ZHANG Tiantao	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
SHI Wangpeng	Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing 100193, China
ZHANG Yongjun	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China	yjzhang@ippcaas.cn

Abstract:To investigate the molecular mechanisms of defense response that Spodoptera frugiperda infestation trigger in maize plants, transcriptome sequencing was performed to analyze the maize plants infested with S. frugiperda larvae, and as yet unknown defensive genes involved in the biosynthesis of important secondary metabolites were identified. The results showed that 1 645 transcripts were differentially expressed (log₂|treatment/control|>1; FDR<0.05) in S. frugiperda-infested (18 h) maize leaves compared to the uninfested control plants. Among these differentially expressed genes (DEGs), 1 352 were significantly upregulated, and 293 were significantly downregulated. Most of the DEGs involved in biosynthesis and signaling pathway of phytohormones such as jasmonic acid (JA), salicylic acid (SA) and ethylene (ET) were upregulated, and interestingly, all 44 DEGs in JA pathways were upregulated, highlighting a central role of JA in S. frugiperda-induced signaling with overlapping and synergistic effects on other phytohormone pathways. In the biosynthesis of benzoxazinoids, the most important defensive secondary metabolites in maize, nine genes were upregulated. Moreover, almost all (14 of 15) DEGs involved in herbivore-induced terpene biosynthesis were upregulated, and more noteworthily, all eight terpene synthase genes and one CYP gene (CYP92C5) responsible for terpenoid volatile emission were upregulated. The present data indicated that S. frugiperda induced massive changes in maize transcriptome and complex crosstalk between phytohormone pathways, and triggered the transcriptional reprogramming of defensive secondary metabolism.

keywords:chemical communication ecological regulation signaling molecule plant volatile terpene synthase

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