| 转Cry1Ab-ma基因玉米CM8101对草地贪夜蛾1龄和2龄幼虫的抗性 |
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| 引用本文：武奉慈,翁建峰,李新海,尹俊琦,宋新元.转Cry1Ab-ma基因玉米CM8101对草地贪夜蛾1龄和2龄幼虫的抗性.植物保护学报,2020,47(4):815-821 |
| DOI：10.13802/j.cnki.zwbhxb.2020.2020818 |
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| 中文摘要:为明确转Bt基因玉米对草地贪夜蛾Spodoptera frugiperda的抗性，采用室内玉米离体组织生测法测定取食转Cry1Ab-ma基因抗虫玉米CM8101心叶、花丝和籽粒后草地贪夜蛾1龄和2龄幼虫存活数量、存活幼虫的体重、蛹重、化蛹和羽化等参数，分析转Cry1Ab-ma基因抗虫玉米CM8101三种组织对草地贪夜蛾1龄和2龄幼虫的抗性。结果显示，转Cry1Ab-ma基因玉米CM8101心叶可完全杀死草地贪夜蛾1龄幼虫，2龄幼虫有存活，存活率为10.0%，但不能化蛹与羽化；花丝可杀死大部分草地贪夜蛾1龄幼虫，存活率为3.3%，并阻断其生活史，但对2龄幼虫杀伤力弱，存活率为73.3%，存活幼虫化蛹率和羽化率分别为20.0%和13.3%；籽粒可完全杀死草地贪夜蛾1龄幼虫，2龄幼虫有存活，存活率为13.3%，有少量可化蛹，化蛹率为3.3%、有少量羽化，羽化率为3.3%。表明转Cry1Ab-ma基因玉米CM8101在短期内对草地贪夜蛾1龄和2龄幼虫有良好的控制作用。 |
| 中文关键词:草地贪夜蛾 转Cry1Ab-ma基因玉米CM8101 抗虫性 室内生测 |
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| Resistance of transgenic maize CM8101 with Cry1Ab-ma gene against the 1st and 2nd instar larvae of fall armyworm Spodoptera frugiperda |
| Author Name | Affiliation | E-mail | | WU Fengci | Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, Jilin Province, China | | | WENG Jianfeng | Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | LI Xinhai | Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China | | | YIN Junqi | Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, Jilin Province, China | yin_junqi@163.com | | SONG Xinyuan | Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, Jilin Province, China | songxinyuan1980@163.com |
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| Abstract:To clarify the resistance effects of transgenic Bt maize against fall armyworm Spodoptera frugiperda, the vitro tissues bioassay was conducted in the laboratory. The 1st and 2nd instar larvae of S. frugiperda were feed with heart leaves, maize silks and seeds, the survival number of 1st and 2nd instar larvae, weight of surviving larvae, pupal weight, number of pupation and eclosion were measured, the resistance of heart leaves, maize silks and seeds of transgenic corn CM8101 with Cry1Ab-ma gene to the 1st and 2nd instar larvae of S. frugiperda were analyzed. The results showed that the 1st instar larvae were completely killed by the heart leaves, and the 2nd instar larvae could survive with survival rate 10.0%, but could not complete the life cycle of pupation and eclosion. The most 1st instar larvae with survival rate of 3.3% could be killed by maize silks and their life cycles were interrupted, however, it was not lethal to 2nd instar larvae with survival rate of 73.3%, and pupation rate was 20.0%, eclosion rate was 13.3%. The 1st instar larvae were completely killed by seeds, and the 2nd instar larvae could survive with survival rate 13.3%, had less pupae with pupation rate of 3.3%, and had less pupae with eclosion rate of 3.3%.The results indicated that the transgenic maize CM8101 with Cry1Ab-ma gene had high control efficacy against the 1st and 2nd instar larvae of fall armyworm, and could effectively block their life cycles and reduce the pest damage. |
| keywords:Spodoptera frugiperda transgenic maize CM8101 with Cry1Ab-ma gene insect resistance laboratory bioassay |
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