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棉花中5-羟色胺含量的时空动态及其对棉铃虫生长发育的影响

引用本文：孙佩瑶,滕东,吕贝贝,张航,张永军.棉花中5-羟色胺含量的时空动态及其对棉铃虫生长发育的影响.植物保护学报,2021,48(5):1075-1080

DOI：10.13802/j.cnki.zwbhxb.2021.2021860

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作者	单位	E-mail
孙佩瑶	中国农业科学院植物保护研究所, 植物病虫害生物学国家实验室, 北京 100193
滕东	中国农业科学院植物保护研究所, 植物病虫害生物学国家实验室, 北京 100193
吕贝贝	中国农业科学院植物保护研究所, 植物病虫害生物学国家实验室, 北京 100193
张航	中国农业科学院植物保护研究所, 植物病虫害生物学国家实验室, 北京 100193
张永军	中国农业科学院植物保护研究所, 植物病虫害生物学国家实验室, 北京 100193	yjzhang@ippcaas.cn

中文摘要:为明确棉花中5-羟色胺含量的时空动态及其对棉铃虫Helicoverpa armigera生长发育的影响，采用高效液相色谱方法检测不同棉花品种（系）中5-羟色胺含量的时空动态，并以添加外源5-羟色胺的人工饲料饲喂棉铃虫，分析5-羟色胺对其生长发育的影响。结果表明，不同棉花品种（系）叶片中5-羟色胺的含量为5.0~20.8 μg/g，其中蕾期与花期棉株叶片中5-羟色胺的含量均高于苗期与铃期棉株叶片，除个别品种（系）外差异均达显著水平。饲喂添加外源5-羟色胺的人工饲料可导致棉铃虫化蛹提前、发育历期缩短以及蛹重增加。10 μg/g和100 μg/g的5-羟色胺对棉铃虫幼虫的死亡率或没有影响或降低死亡率，但是极高浓度（500 μg/g和1 000 μg/g）的5-羟色胺能够提高棉铃虫幼虫的死亡率。表明常规棉花蕾期与花期较高含量的5-羟色胺可能有利于棉铃虫的生长发育，在抗虫品种（系）培育中可将低5-羟色胺含量作为抗虫种质资源的参考指标。

中文关键词:棉花 5-羟色胺时空动态抗虫性

Temporal and spatial dynamics of 5-hydroxytryptamine content in cotton and its effects on the growth and development of cotton bollworm Helicoverpa armigera

Author Name	Affiliation	E-mail
Sun Peiyao	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Teng Dong	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Lü Beibei	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Zhang Hang	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Zhang Yongjun	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China	yjzhang@ippcaas.cn

Abstract:In order to clarify the temporal and spatial dynamics of 5-hydroxytryptamine (5-HT) content in cotton and its effects on the growth and development of cotton bollworm Helicoverpa armigera, a high performance liquid chromatography (HPLC) method was established for detecting 5-HT in cotton. Based on the HPLC method, the temporal and spatial dynamics of 5-HT content in different varieties (lines) of cotton were determined. In addition, exogenous 5-HT was added to artificial diets to feed larvae of cotton bollworm, and its effect on the growth and development of this insect was investigated. The results showed that the content of 5-HT in cotton leaves ranged from 5.0 μg/g to 20.8 μg/g, and the contents during budding stage and flowering stage were significant higher than those during seedling and boll-setting stages. Addition of exogenous 5-HT resulted in earlier pupation of the cotton bollworm, shortened development period and increased pupal weight. The 5-HT at 10 μg/g and 100 μg/g did not affect or reduce the mortality of cotton bollworm larvae, but extremely high concentrations of 5-HT (500 μg/g and 1 000 μg/g) increased the mortality of cotton bollworm larvae. The study indicated that the higher content of 5-HT at the budding and flowering stage of conventional cotton might be beneficial to the growth and development of cotton bollworm. In the breeding of insect-resistant varieties (lines), lower 5-HT content can be used as a reference index for insect-resistant germplasm resources.

keywords:cotton 5-hydroxytryptamine temporal and spatial dynamics insect resistance

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