吉林省西瓜蔓枯病菌对吡唑醚菌酯的抗性监测及抗性风险评估 |
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引用本文:孙东晗,陈悦,田文学,白庆荣,杨丽娜,赵廷昌.吉林省西瓜蔓枯病菌对吡唑醚菌酯的抗性监测及抗性风险评估.植物保护学报,2022,49(6):1663-1672 |
DOI:10.13802/j.cnki.zwbhxb.2022.2021004 |
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中文摘要:为明确吉林省西瓜蔓枯病菌Stagonosporopsis citrulli对吡唑醚菌酯的敏感基线和抗性风险,采用菌丝生长速率法测定吉林省151株西瓜蔓枯病菌菌株对吡唑醚菌酯的敏感性及吡唑醚菌酯对西瓜蔓枯病菌的最低抑制浓度,并监测375株西瓜蔓枯病菌菌株对吡唑醚菌酯的抗性水平。结果表明,供试的151株菌株对吡唑醚菌酯的敏感性频率分布呈连续的单峰曲线,接近正态分布,敏感基线为1.800 μg/mL;吡唑醚菌酯的最低抑制浓度MIC为80.000 μg/mL。抗性水平监测结果显示,375株菌株对吡唑醚菌酯的抗性频率为0。室内药剂驯化获得的抗性突变菌株T-LH3-1和T-XZC5-1在连续转接培养8代后的EC50分别为16.139 μg/mL和31.782 μg/mL,抗性倍数分别为15和51,均为中抗水平,抗药性可以稳定遗传。抗性突变体及其亲本菌株生物学性状结果显示,抗性突变体生物适合度与其亲本菌株差异不显著。交互抗性结果表明,吡唑醚菌酯与醚菌酯之间存在正交互抗性,与戊唑醇、异菌脲、多菌灵和肟菌酯之间不存在交互抗性,可以交替使用以达到延缓抗药性的产生。 |
中文关键词:西瓜蔓枯病菌 吡唑醚菌酯 抗性监测 抗性风险 |
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Monitoring and risk evaluation of the resistance to pyraclostrobin in gummy stem blight pathogen Stagonosporopsis citrulli from watermelon in Jilin Province |
Author Name | Affiliation | E-mail | Sun Donghan | College of Plant protecion, Jilin Agricultural University, Changchun 130118, Jilin Province, China | | Chen Yue | College of Plant protecion, Jilin Agricultural University, Changchun 130118, Jilin Province, China Agricultural and RuralService Center in Heihe, Heihe 164300, Heilongjiang Province, China | | Tian Wenxue | Shandong Sino-Agri United Biotechnology Co., Ltd., Jinan 250100, Shandong Province, China | | Bai Qingrong | College of Plant protecion, Jilin Agricultural University, Changchun 130118, Jilin Province, China | | Yang Lina | College of Plant protecion, Jilin Agricultural University, Changchun 130118, Jilin Province, China | yanglina20140@126.com | Zhao Tingchang | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute ofPlant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | zhaotgcg@163.com |
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Abstract:To evaluate the resistance to pyraclostrobin in Stagonosporopsis citrulli causing gummy stem blight of Citrullus lanatus in Jilin Province, the sensitivity baseline and the minimum inhibitory concentration (MIC) to pyraclostrobin were determined for 151 S. citrulli strains collected from watermelon in Jilin Province based on mycelial growth rate. Meanwhile, the resistance levels to pyraclostrobin were also monitored for other 375 watermelon strains from Jilin. The results showed that the sensitivity frequency distribution of tested strains (n=151) was a continuous single peak curve close to a normal distribution. The sensitivity baseline was 1.800 μg/mL, and the MIC was 80.000 μg/mL to pyraclostrobin for these strains. The resistance frequency of the 375 tested strains to pyraclostrobin was 0. Two intermediate pyraclostrobin-resistant mutants (T-LH3-1 and T-XZC5-1) were obtained by chemical taming. After continuously culturing without the fungicide for eight generations, EC50 of T-LH3-1 and and T-XZC5-1, were 16.139 μg/mL and 31.782 μg/mL, respectively, and their resistance were 15 and 51 times of the resistance of their parents, respectively, remaining to be comparable to their parents, indicating that they still kept the intermediate resistance, and their resistance could be inherited stably. Comparison of the biological characteristics of both intermediate resistant mutants with their parental strains indicated that the biocompatibility of the resistant mutants were not significantly different from their parent strains. Cross-resistance results showed that there was a positive cross-resistance between pyraclostrobin and kresoxim-methyl, but there was no cross-resistance between pyraclostrobin and tebuconazole, iprodione, carbendazim, and trifloxystrobin, indicating that they could be used alternately so as to delay the development of resistance. |
keywords:Stagonosporopsis citrulli pyraclostrobin resistant detection risk of resistance |
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