| 链格孢菌橘致病型侵染柑橘抗感品种的转录组差异表达分析 |
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| 引用本文：钟胤珊,张懿丹,唐科志,周常勇.链格孢菌橘致病型侵染柑橘抗感品种的转录组差异表达分析.植物保护学报,2025,52(6):1379-1391 |
| DOI：10.13802/j.cnki.zwbhxb.2025.2025033 |
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| 中文摘要:为探究柑橘褐斑病致病菌链格孢菌橘致病型（the tangerine pathotype of Alternaria alternata）在侵染柑橘抗感品种过程中的基因表达差异，利用其菌丝球分别侵染感病品种红橘和抗病品种纽荷尔脐橙后，采用高通量测序技术对病原菌菌丝阶段（0 h）及3个侵染时期（接种后12、24和48 h）的样品进行转录组测序与组装，并进行功能注释与分类、差异基因筛选与分析。结果显示：链格孢菌橘致病型侵染感病品种红橘后其特异性差异表达基因的功能主要富集于细胞能量代谢、氨基酸和辅酶的合成与分解以及果胶的降解过程，该菌侵染抗病品种纽荷尔脐橙后其特异性差异表达基因的功能主要富集于细胞结构构建、细胞通信以及环境应激和响应，说明链格孢菌橘致病型侵染红橘和纽荷尔脐橙时所激活的生物反应和代谢途径不同。将该菌接种于红橘离体叶片后，其通过协同调控毒力、代谢及生长发育等过程主动侵染寄主，抑制寄主防御反应，实现在寄主体内的高效寄生适应；而将其接种于纽荷尔脐橙离体叶片后，病原菌与寄主的防御系统一直呈现博弈的状态，该菌也因寄主持续的防御干扰导致其一直未能完全瓦解寄主抗性，致使无法成功侵染定殖。实时荧光定量PCR验证结果与转录组测序结果一致，表明转录组测序数据可靠。 |
| 中文关键词:链格孢菌橘致病型 抗感品种 转录组 差异表达基因 |
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| Transcriptome differential expression analysis of the tangerine pathotype of Alternaria alternata after infecting citrus resistant/susceptible varieties |
| Author Name | Affiliation | E-mail | | Zhong Yinshan | Citrus Research Institute, Southwest University, Chongqing 400712, China | | | Zhang Yidan | Citrus Research Institute, Southwest University, Chongqing 400712, China | | | Tang Kezhi | Citrus Research Institute, Southwest University, Chongqing 400712, China | kztang@swu.edu.cn | | Zhou Changyong | Citrus Research Institute, Southwest University, Chongqing 400712, China | zhoucy@cric.cn |
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| Abstract:To explore the differences in gene expression of the tangerine pathotype of Alternaria alternata during the infection of citrus resistant/susceptible varieties, the susceptible variety Hongju and the resistant variety Newhall navel orange were inoculated with mycelial pellets of the tangerine pathotype of A. alternata, respectively. The high-throughput sequencing technology was used to sequence and assemble the transcriptomes of samples at the mycelial stage of the pathogen（0 h） and during three infection time points（12, 24, and 48 hours post-inoculation）. Additionally, functional annotation and classification were performed, along with differential gene screening and analysis. The results showed that the functions of the specifically differentially expressed genes（DEGs） of the tangerine pathotype of A. alternata after infecting Hongju were mainly enriched in cellular energy metabolism, the synthesis and decomposition of amino acids and coenzymes, and the pectin degradation process. The functions of the DEGs of this pathogen after infecting Newhall navel orange were mainly enriched in cell structure construction, cell communication, and environmental stress response. These results indicated the differences in the biological responses and metabolic pathways activated by the tangerine pathotype of A. alternata when infecting Hongju and Newhall navel orange. When the tangerine pathotype of A. alternata was inoculated onto detached leaves of Hongju tangerine, it actively infects the host by coordinating the regulation of virulence, metabolism, growth and development processes, thereby suppressing the defense response and achieving efficient parasitism adaptation within the host. When the tangerine pathotype of A. alternata was inoculated onto detached leaves of Newhall navel orange, the pathogen and the host’s defense systems remained in a state of constant interaction. Due to the continuous defense interference from the host, the pathogen was unable to completely break down the host’s resistance, this results in the inability of the mycelium to successfully infect and colonize. The real-time quantitatire PCR verification results were consistent with the transcriptional sequencing results, indicating that the transcriptome sequencing data was reliable. |
| keywords:the tangerine pathotype of Alternaria alternata resistant/susceptible varieties transcriptome differentially expressed gene |
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