| 番茄品种对细菌性溃疡病的抗性及其抗病相关指标分析 |
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| 引用本文：张舒涵,刘朋飞,仇智灵,楼兵干.番茄品种对细菌性溃疡病的抗性及其抗病相关指标分析.植物保护学报,2025,52(6):1392-1403 |
| DOI：10.13802/j.cnki.zwbhxb.2025.2025068 |
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| 中文摘要:为明确不同番茄品种对细菌性溃疡病的抗性水平，发掘番茄细菌性溃疡病抗性品种并解析其抗病机制，采用针刺接种法对66个番茄品种进行细菌性溃疡病抗性评价，并对高抗、高感番茄品种接种番茄细菌性溃疡病菌——密执安棒杆菌Clavibacter michiganensis后的抗氧化酶活性、丙二醛（malondialdehyde,MDA）含量及抗病相关基因表达水平进行测定。结果表明：66个番茄品种对细菌性溃疡病的抗性可分为5个等级，其中，Micro Tom表现为高抗，红白兰地表现为抗病，釜山99、白灵千禧和小千金黄妃等23个品种表现为中抗，普罗旺斯、牛心番茄和黑美人等27个品种表现为感病，黄洋梨、迷彩番茄和合作909等14个品种表现为高感。按抗病的表现形式将供试番茄品种分为3种类型，即抗侵入类型品种21个，抗扩展类型品种12个，双敏感类型品种33个。接种番茄细菌性溃疡病菌后高抗品种Micro Tom的过氧化物酶（peroxidase,POD）、超氧化物歧化酶（superoxide dismutase,SOD）和过氧化氢酶（catalase,CAT）活性均显著高于接种后的高感品种合作909，接种后72 h高抗品种的POD和CAT活性分别是高感品种的2.28倍和1.40倍，接种后120 h高抗品种的SOD活性是高感品种的1.72倍，而接种后高抗品种的MDA含量则显著低于高感品种的。通过定时荧光定量PCR检测发现番茄细菌性溃疡病菌侵染可诱导高抗品种病程相关蛋白编码基因LePOD和PR-NP24显著上调表达。表明番茄品种对细菌性溃疡病菌的抗性与其抗氧化能力、膜脂过氧化水平和病程相关基因表达水平密切相关。 |
| 中文关键词:番茄细菌性溃疡病 密执安棒杆菌 品种 抗性评价 抗性指标 |
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| Evaluation of resistance in tomato varieties to bacterial canker and analysis of disease resistance-related indicators |
| Author Name | Affiliation | E-mail | | Zhang Shuhan | Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China | | | Liu Pengfei | Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China | | | Qiu Zhiling | Hangzhou Lin'an Plant Quarantine Station, Hangzhou 311300, Zhejiang Province, China | | | Lou Binggan | Ministry of Agriculture and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insect Pests, Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China | bglou@zju.edu.cn |
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| Abstract:In order to clarify the resistance levels of different tomato varieties to bacterial canker, identify resistant tomato varieties, and elucidate the underlying resistance mechanisms, a needle-puncture inoculation method was used to evaluate the resistance of 66 tomato varieties to this disease. The activities of antioxidant enzymes, malondialdehyde（MDA） content, and the expression levels of disease resistance-related genes were measured in highly resistant and highly susceptible tomato varieties after inoculation with Clavibacter michiganensis, the pathogen causing tomato bacterial canker. The results showed that the resistance of the 66 tomato varieties to bacterial canker could be classified into five grades: Micro Tom was highly resistant, Red Brandy resistant, 23 varieties including Fushan 99, Bailingqianxi, and Xiaoqianjinhuangfei moderately resistant, 27 varieties including Provence, Niuxin Tomato, and Heimeiren susceptible, and 14 varieties including Yellow Pear, Micai Tomato, and Hezuo 909 highly susceptible. Based on the manifestation of resistance, the tested tomato varieties were divided into three types: 21 varieties were resistant to pathogen invasion, 12 varieties resistant to pathogen expansion, and 33 varieties dual-sensitive. The activities of peroxidase（POD）, superoxide dismutase（SOD）, and catalase（CAT） in the highly resistant variety Micro Tom inoculated with C. michiganensis were significantly higher than those in the highly susceptible variety Hezuo 909. At 72 hours post-inoculation, the POD and CAT activities of the highly resistant variety were 2.28 times and 1.40 times those of the highly susceptible variety, respectively. At 120 hours post-inoculation, the SOD activity of the highly resistant variety was 1.72 times that of the highly susceptible variety, while the MDA content of the highly resistant variety was significantly lower than that of the highly susceptible variety. Real-time quantitative RCR analysis indicated that infection by C. michiganensis induced significant upregulation of the pathogenesis-related protein-encoding genes LePOD and PR-NP24 in the highly resistant variety. These findings demonstrate that the resistance of tomato varieties to C. michiganensis is closely associated with their antioxidant capacity, membrane lipid peroxidation level, and the expression levels of pathogenesis-related genes. |
| keywords:tomato bacterial canker Clavibacter michiganensis variety resistance evaluation resistance index |
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