欢迎访问《植物保护学报》网站！

首页 | 期刊简介 |
编委会
编委会
青年编委会
| 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English

江苏省洁田稻种植区稗草对甲氧咪草烟的抗性水平及抗性机制

引用本文：凌溪铁,仇泽宇,唐兆成,杨霞,张保龙.江苏省洁田稻种植区稗草对甲氧咪草烟的抗性水平及抗性机制.植物保护学报,2025,52(6):1429-1437

DOI：10.13802/j.cnki.zwbhxb.2025.2025017

摘要点击次数:

全文下载次数:

作者	单位	E-mail
凌溪铁	江苏省农业科学院种质资源与生物技术研究所, 江苏省农业生物学重点实验室, 南京 210014
仇泽宇	江苏省农业科学院种质资源与生物技术研究所, 江苏省农业生物学重点实验室, 南京 210014
唐兆成	江苏省农业科学院种质资源与生物技术研究所, 江苏省农业生物学重点实验室, 南京 210014
杨霞	江苏省农业科学院种质资源与生物技术研究所, 江苏省农业生物学重点实验室, 南京 210014
张保龙	江苏省农业科学院种质资源与生物技术研究所, 江苏省农业生物学重点实验室, 南京 210014	zhbl2248@hotmail.com

中文摘要:为明确耐甲氧咪草烟水稻田中稗草的抗性情况和抗性机制，2023年10月自南京市江北新区连续种植6年以上抗甲氧咪草烟水稻金粳818的田块中采集西来稗Echinochloa crus-galli var.zelayensis、无芒稗E. crus-galli var. mitis、硬稃稗E. glabrescens和稗E. crus-galli var. crus-galli，采用整株剂量反应试验测定这4个种群对甲氧咪草烟的敏感性，并测定其对甲氧咪草烟、双草醚和五氟磺草胺3种乙酰乳酸合成酶（acetolactate synthase,ALS）抑制剂类除草剂的抗性水平，并通过靶标基因ALS测序和ALS活性变化初步探索其靶标抗性机制。结果显示：西来稗、无芒稗、硬稃稗和稗4个稗草种群对甲氧咪草烟均产生了抗性，其抗性指数分别为32.92、25.27、33.95和21.35；且这4个抗性稗草种群对双草醚和五氟磺草胺产生不同水平的交互抗性，其中对双草醚的抗性指数分别为11.03、13.17、13.71和13.89，对五氟磺草胺的抗性指数分别为29.56、25.32、34.03和28.51。这4个抗性稗草种群ALS3中第549位氨基酸由色氨酸Trp突变为亮氨酸Leu。45 g/hm²甲氧咪草烟处理3 d后，硬稃稗、西来稗、无芒稗和稗敏感种群的ALS活性分别为0.77、0.68、0.83和0.72 U，均较处理前显著下降；硬稃稗、西来稗、无芒稗和稗抗性种群的ALS活性分别为1.81、1.76、1.89和1.86 U，均较处理前显著下降，且显著高于其对应敏感种群。综上所述，ALS3基因突变是4种稗草对甲氧咪草烟产生抗性的主要原因之一，并同时赋予其对其他ALS类除草剂的交互抗性。

中文关键词:稗西来稗无芒稗硬稃稗甲氧咪草烟乙酰乳酸合成酶基因突变抗性水平

Resistance levels and underlying mechanisms of barnyard grasses Echinochloa spp. from clearfield rice cultivation area in Jiangsu Province to imazamox

Author Name	Affiliation	E-mail
Ling Xitie	Jiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu Province, China
Qiu Zeyu	Jiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu Province, China
Tang Zhaocheng	Jiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu Province, China
Yang Xia	Jiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu Province, China
Zhang Baolong	Jiangsu Provincial Key Laboratory of Agrobiology, Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, Jiangsu Province, China	zhbl2248@hotmail.com

Abstract:In order to clarify the status and mechanisms of resistance in Echinochloa spp. to imazamox in imazamox-resistant rice fields, four ecotypes of Echinochloa spp. populations（E. crus-galli var. zelayensis, E. crus-galli var. mitis, E. glabrescens and E. crus-galli var. crus-galli） were collected in October 2023 from paddy fields in Jiangbei New District, Nanjing, Jiangsu, where the imazamoxresistant rice Jinjing 818 had been cultivated continuously for more than six years. Whole-plant doseresponse assays were performed to determine the sensitivity of these populations to imazamox and to evaluate their cross-resistance levels to two other acetolactate synthase（ALS） inhibitors: bispyribacsodium and penoxsulam. Furthermore, the target-site resistance mechanism was preliminarily investigated by sequencing the ALS gene and analyzing the corresponding enzyme activity. The results showed that E. crus-galli var. zelayensis, E. crus-galli var. mitis, E. glabrescens and E. crus-galli var. crus-galli have developed resistance to imazamox, with resistance indices of 32.92, 25.27, 33.95, and 21.35, respectively. Furthermore, these four resistant populations exhibited varying levels of crossresistance to bispyribac-sodium and penoxsulam. Their resistance indices to bispyribac-sodium were 11.03, 13.17, 13.71, and 13.89, respectively, and to penoxsulam were 29.56, 25.32, 34.03, and 28.51, respectively. Sequencing analysis revealed a point mutation in the ALS3 gene of all four resistant populations, the amino acid at position 549 was mutated from tryptophan（Trp） to leucine（Leu）. Three days after treatment with imazamox at 45 g/hm², the ALS activity in susceptible populations（0.77, 0.68, 0.83, 0.72 U） was significantly lower than in resistant populations（1.81, 1.76, 1.89, 1.86 U） for E. glabrescens, E. crus-galli var. zelayensis, E. crus-galli var. mitis and E. crus-galli, respectively, although all showed a significant decrease compared with pre-treatment levels. In conclusion, the ALS3 gene mutation（Trp-549-Leu） is a primary mechanism conferring imazamox resistance in these four Echinochloa ecotypes and simultaneously confers cross-resistance to other ALS-inhibiting herbicides.

keywords:Echinochloa crus-galli var. zelayensis Echinochloa crus-galli var. mitis Echinochloa gla-brescens Echinochloa crus-galli var. crus-galli imazamox acetolactate synthase （ALS） gene mutation resistance level

查看全文查看/发表评论下载PDF阅读器