| 湖南省张家界市莓茶早疫病病原菌鉴定及防治药剂筛选 |
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| 引用本文：周衍章,凌云,张圆,罗漫,赵剑,许文祺,刘宇知,周泽华,易图永.湖南省张家界市莓茶早疫病病原菌鉴定及防治药剂筛选.植物保护学报,2025,52(6):1447-1456 |
| DOI：10.13802/j.cnki.zwbhxb.2025.2025078 |
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| 中文摘要:为有效防控莓茶早疫病，从张家界市不同莓茶种植园采集具有典型症状的病叶，对其进行分离纯化，通过形态学特征与分子生物学手段对分离菌株进行鉴定，初步测定8类常用杀菌剂对该病原菌的抑制效果。结果显示：经过分离纯化共获得100株形态相似的菌株；在PDA平板上分离菌株的菌落中央呈灰褐色，边缘呈灰白色，气生菌丝绒状；分生孢子呈卵圆形或倒棍棒形，具9～11个横膈膜和0～3个纵膈膜，平均大小约为21.9μm×10.1μm。代表菌株B1、B3和B4的ITS、ALT和GAPDH序列与互隔交链孢菌Alternaria alternata的相似性均高于96%，结合形态学特征将其鉴定为互隔交链孢菌A. alternata。在8类共14种杀菌剂中，咯菌腈对分离菌株B1和B3均有较高的抑制效果，EC50分别为0.144μg/mL和0.152μg/mL。因此，咯菌腈可作为防治由互隔交链孢菌引起的莓茶早疫病的候选药剂。 |
| 中文关键词:莓茶 早疫病 病原菌 互隔交链孢菌 抑菌活性 防治药剂 形态学 鉴定 |
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| Identification of the pathogen causing vine tea early blight in Zhangjiajie, Hunan Province and screening of fungicides |
| Author Name | Affiliation | E-mail | | Zhou Yanzhang | College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China | | | Ling Yun | College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China | | | Zhang Yuan | College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China | | | Luo Man | Agriculture and Rural Affairs Bureau of Yongding District, Zhangjiajie 427099, Hunan Province, China | | | Zhao Jian | Agriculture and Rural Affairs Bureau of Yongding District, Zhangjiajie 427099, Hunan Province, China | | | Xu Wenqi | Huaihua Vocational Technical College, Huaihua 418099, Hunan Province, China | | | Liu Yuzhi | National Agricultural Science and Technology Management Center of Zhangjiajie, Zhangjiajie 427000, Hunan Province, China | | | Zhou Zehua | College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China | m15895982813@163.com | | Yi Tuyong | College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China | yituyong@hunau.net |
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| Abstract:To effectively control vine tea early blight, diseased leave with typical symptoms were collected from different vine tea plantations in Zhangjiajie, Hunan Province. The causal agents were isolated and purified, and identified based on morphological characteristics and molecular assays. The inhibitory effects of eight commonly used fungicides against the pathogens were preliminarily determined. The results showed that 100 isolates with similar morphological characteristics were obtained. All colonies were initially grey-white, and turned into dark-gray with abundant cotton-shaped aerial mycelia on potato dextrose agar（PDA） plates. The conidia were ovoid or inverted club-shaped, and measured 21.9 μm×10.1 μm, 0-3 longitudinal septa and 9-11 septa per conidium. The ITS, ALT, and GAPDH sequences of representative strains B1, B3, and B4 exhibited a similarity of over 96% to those of the fungal pathogen Alternaria alternata. Combined with their morphological characteristics, these strains were identified as A. alternata. Among the 14 fungicides belonging to eight categories, fludioxonil exhibited a higher inhibitory effect against strains B1 and B3 compared with other selected fungicides, with the EC50 values of 0.144 μg/mL and 0.152 μg/mL respectively. Therefore, fludioxonil can be used as the candidate agent for the control of vine tea early blight caused by A. alternata. |
| keywords:vine tea early blight pathogen Alternaria alternata antifungal activity control agent morphology identification |
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