| 低温贮存对白星花金龟3龄幼虫存活、能量分配及抗氧化酶活性的影响 |
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| 引用本文：李彦萍,黄勋,马德英.低温贮存对白星花金龟3龄幼虫存活、能量分配及抗氧化酶活性的影响.植物保护学报,2025,52(6):1467-1476 |
| DOI：10.13802/j.cnki.zwbhxb.2025.2025059 |
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| 中文摘要:为解决白星花金龟Protaetia brevitarsis幼虫生命周期与生产需求的时间错配问题，以其3龄幼虫为研究对象，测定不同贮存温度（4、8、12、25℃）与不同贮存时间（10、20、30 d）下该试虫的存活率、体内能源物质（总蛋白、粗脂肪、总糖、总氨基酸）含量、抗寒代谢物海藻糖含量及抗氧化酶系统超氧化物歧化酶（superoxide dismutase,SOD）、过氧化氢酶（catalase,CAT）、过氧化物酶（peroxidase,POD）的活性变化。结果表明：12℃为白星花金龟3龄幼虫最适贮存温度，12℃贮存30 d后3龄幼虫的存活率仍保持在88.33%，而4℃和8℃贮存30 d后3龄幼虫的存活率显著降低，分别为24.17%和51.67%。12℃贮存20 d时白星花金龟3龄幼虫体内海藻糖、总糖与总蛋白含量均达到峰值，SOD活性也在同期显著升高；而在4℃和8℃低温胁迫下，多数能量物质代谢受到抑制，抗氧化酶系统协调性丧失，SOD、POD与CAT活性呈紊乱响应，机体清除自由基能力下降。表明在12℃下白星花金龟3龄幼虫通过积极积累抗寒物质并维持抗氧化系统稳定来适应低温环境，而更低温度导致的死亡与能量代谢失衡及保护酶系统功能破坏密切相关。 |
| 中文关键词:白星花金龟 3龄幼虫 低温贮存 贮存温度 贮存时间 代谢调控 抗氧化酶 |
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| Effect of cold storage on survival, energy metabolism, and antioxidant enzyme activities in third-instar larvae of white-spotted flower chafer Protaetia brevitarsis |
| Author Name | Affiliation | E-mail | | Li Yanping | Key Laboratory of Monitoring and Safety Control of Harmful Organisms in Agriculture and Forestry, College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang Uygur Autonomous Region, China | | | Huang Xun | Key Laboratory of Monitoring and Safety Control of Harmful Organisms in Agriculture and Forestry, College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang Uygur Autonomous Region, China | | | Ma Deying | Key Laboratory of Monitoring and Safety Control of Harmful Organisms in Agriculture and Forestry, College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang Uygur Autonomous Region, China | mdyxnd@163.com |
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| Abstract:To resolve the temporal mismatch between the larval life cycle of white-spotted flower chafer Protaetia brevitarsis and industrial production needs, this study investigated how different cold-storage temperatures（4, 8, 12, and 25 ℃） and durations（10, 20, and 30 d） affect the survival rate of third-instar larvae. Measurements included survival rate, energy reserves（total protein, crude fat, total sugar, and total amino acid）, the cryoprotectant trehalose, and the activities of major antioxidant enzymes, including superoxide dismutase（SOD）, catalase（CAT）, and peroxidase（POD）. The results showed that 12 ℃ was the optimal storage temperature, at which larval survival remained as high as 88.33% after 30 days. In contrast, storage at 4 ℃ and 8 ℃ significantly reduced survival, declining to 24.17% and 51.67%, respectively. After 20 days at 12 ℃, larvae exhibited peak levels of trehalose, total sugar, and total protein, accompanied by a significant elevation in SOD activity. However, under greater cold stress（4 ℃ and 8 ℃）, the metabolism of most energy substances was inhibited, and the coordination of the antioxidant enzyme system was disrupted. The disordered responses of SOD, POD, and CAT resulted in weakened free radical scavenging capacity. These findings indicate that third-instar larvae of P. brevitarsis adapt to mild low-temperature storage（12 ℃） by actively accumulating cryoprotectants and maintaining antioxidant homeostasis, whereas mortality at lower temperatures is closely associated with energymetabolism imbalance and impairment of protective enzyme function. |
| keywords:Protaetia brevitarsis third instar larva cold storage storage temperature storage duration metabolic regulation antioxidant enzyme |
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