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基于转录组测序解析麦红吸浆虫对小麦防御物质解毒代谢的分子机制

引用本文：张国军,陈锐,金绮梦,王兴云,成卫宁.基于转录组测序解析麦红吸浆虫对小麦防御物质解毒代谢的分子机制.植物保护学报,2026,53(1):198-208

DOI：10.13802/j.cnki.zwbhxb.2026.2025096

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作者	单位	E-mail
张国军	西北农林科技大学植物保护学院, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
陈锐	西北农林科技大学植物保护学院, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
金绮梦	西北农林科技大学植物保护学院, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
王兴云	西北农林科技大学植物保护学院, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100
成卫宁	西北农林科技大学植物保护学院, 植保资源与病虫害治理教育部重点实验室, 陕西杨凌 712100	cwning@126.com

中文摘要:为筛选麦红吸浆虫Sitodiplosis mosellana参与解毒代谢小麦防御性次生物质的关键基因，以分别取食阿魏酸等次生物质含量差异显著的高抗小麦品种晋麦47和高感小麦品种西农822的麦红吸浆虫幼虫为材料，采用BGISEQ-500高通量测序平台对其进行转录组测序，分析差异表达基因并对其进行GO分类和KEGG通路富集分析，筛选在麦红吸浆虫解毒代谢中起关键作用的相关基因；采用实时荧光定量PCR技术对部分差异表达基因的表达水平进行验证。结果显示：取食两个小麦品种的麦红吸浆虫幼虫转录组共获得42 505条unigene，其中差异表达基因3 566个，2 188个在取食抗虫小麦的麦红吸浆虫幼虫中显著上调，大多数基因参与细胞进程和代谢进程，具有结合、催化和转运蛋白酶的活性，在有毒物质降解与代谢途径显著富集。在取食抗虫小麦的麦红吸浆虫幼虫中共筛选到 26 个显著上调的解毒酶基因，其中 6 个谷胱甘肽-S-转移酶（glutathione S-transferase，GST）、7 个细胞色素 P450 酶系（cytochrome P450，CYP450）、4 个尿苷二磷酸糖基转移酶（UDPglycosyl transferase，UGT）和9个ATP结合盒（ATP-binding cassette，ABC）转运蛋白基因。随机选取的13个差异表达解毒酶基因的实时荧光定量PCR结果的表达趋势与转录组测序结果基本一致。表明筛选获得的GST、CYP450、UGT和ABC转运蛋白基因可能在麦红吸浆虫对小麦防御性次生物质的解毒代谢和适应抗性寄主防御中起着重要作用。

中文关键词:麦红吸浆虫植物次生物质转录组解毒代谢防御相关基因

Transcriptome analysis reveals the molecular mechanisms of detoxification metabolism of red wheat blossom midge Sitodiplosis mosellana in response to wheat defensive compounds

Author Name	Affiliation	E-mail
Zhang Guojun	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Chen Rui	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Jin Qimeng	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Wang Xingyun	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China
Cheng Weining	Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China	cwning@126.com

Abstract:To identify key genes involved in the detoxification metabolism of wheat defensive secondary metabolites in red wheat blossom midge Sitodiplosis mosellana, larvae feeding on a highly resistant wheat cultivar Jinmai 47 and a highly susceptible wheat cultivar (Xinong 822), which differ significantly in the contents of defensive secondary compounds (e.g. ferulic acid), were subjected to comparative transcriptome sequencing using the BGISEQ 500 platform. Differentially expressed genes (DEGs) were identified and analyzed by Gene Ontology classification and Kyoto Encyclopedia of Genes and Genomes pathway enrichment to screen genes potentially involved in detoxification metabolism. In addition, the expression levels of selected DEGs were validated by real-time quantitative PCR (RTqPCR). A total of 42 505 unigenes were obtained from the combined transcriptomes, among which 3 566 genes were differentially expressed. Compared with larvae fed on the susceptible wheat cultivar, 2 188 DEGs were significantly upregulated in larvae fed on the resistant cultivar. Most of these genes were associated with cellular and metabolic processes and were enriched in functions related to binding, catalytic activity, and transport, with significant enrichment in pathways involved in the degradation and metabolism of toxic substances. A total of 26 detoxification enzyme genes were significantly upregulated in larvae fed on resistant wheat, including six glutathione S-transferases (GSTs), seven cytochrome P450 monooxygenases (CYP450s), four UDP-glycosyltransferases (UGTs) and nine ATPbinding cassette (ABC) transporter genes. RT-qPCR analysis of 13 randomly selected DEGs showed expression patterns largely consistent with the transcriptome sequencing results. These findings suggest that the identified GST, CYP450, UGT, and ABC transporter genes may play pivotal roles in the detoxification metabolism of wheat defensive secondary metabolites and in the adaptation of S. mosellana to resistant host plants.

keywords:Sitodiplosis mosellana plant secondary metabolite transcriptome detoxification metabolism defense-related gene

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