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河北省小麦田麦瓶草对苯磺隆的抗性机制

引用本文：薛鑫慧,户申奥,董佳博,丁晓菲,韦庆康,马红,黄兆峰.河北省小麦田麦瓶草对苯磺隆的抗性机制.植物保护学报,2026,53(1):273-282

DOI：10.13802/j.cnki.zwbhxb.2026.2025092

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作者	单位	E-mail
薛鑫慧	东北农业大学植物保护学院, 哈尔滨 150030 中国农业科学院植物保护研究所, 北京 100193
户申奥	东北农业大学植物保护学院, 哈尔滨 150030 中国农业科学院植物保护研究所, 北京 100193
董佳博	东北农业大学植物保护学院, 哈尔滨 150030 中国农业科学院植物保护研究所, 北京 100193
丁晓菲	中国农业科学院植物保护研究所, 北京 100193
韦庆康	中国农业科学院植物保护研究所, 北京 100193
马红	东北农业大学植物保护学院, 哈尔滨 150030	157492795@qq.com
黄兆峰	中国农业科学院植物保护研究所, 北京 100193	huangzhaofeng@caas.cn

中文摘要:为明确河北省小麦田麦瓶草Silene conoidea抗性种群对苯磺隆的抗性机制，通过整株生物法测定、靶标乙酰乳酸合成酶（acetolactate synthase，ALS）基因序列分析、ALS活性测定、代谢酶抑制试验和交互抗性及多抗性测定进行分析。结果表明：苯磺隆对麦瓶草DZ-2R抗性种群的生长抑制中量GR₅₀为527.60 g（a.i.）/hm²，对S2400敏感种群的GR₅₀为3.99 g（a.i.）/hm²，DZ-2R抗性种群的抗性指数达132.23，对苯磺隆处于高抗水平；ALS基因序列分析发现DZ-2R抗性种群第574位氨基酸由色氨酸（TGG）突变为亮氨酸（TTG），且ALS基因的相对拷贝数和相对表达量在抗性和敏感种群中均无显著差异；苯磺隆对DZ-2R抗性种群的半数抑制浓度I50为211.50 μmol/L，是其对S2400敏感种群的19.71倍；细胞色素P450酶抑制剂马拉硫磷和谷胱甘肽-S-转移酶抑制剂NBD-Cl预处理分别使苯磺隆对 DZ-2R 抗性种群的 GR₅₀下降了 53.43% 和 60.48%；交互抗性和多抗性试验发现DZ-2R抗性种群对甲基二磺隆、双氟磺草胺和双草醚等ALS抑制剂表现出交互抗性，但对双唑草酮和唑草酮表现敏感。综上表明麦瓶草DZ-2R种群对苯磺隆的抗性是由ALS基因突变与代谢解毒能力增强协同所致。

中文关键词:麦瓶草苯磺隆乙酰乳酸合成酶除草剂抗性抗性机制

Mechanisms of tribenuron-methyl resistance in weed Silene conoidea from wheat fields in Hebei Province

Author Name	Affiliation	E-mail
Xue Xinhui	College of Plant Protection, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China Institute of Plant Protecton, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Hu Shen'ao	College of Plant Protection, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China Institute of Plant Protecton, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Dong Jiabo	College of Plant Protection, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China Institute of Plant Protecton, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Ding Xiaofei	Institute of Plant Protecton, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Wei Qingkang	Institute of Plant Protecton, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Ma Hong	College of Plant Protection, Northeast Agricultural University, Harbin 150030, Heilongjiang Province, China	157492795@qq.com
Huang Zhaofeng	Institute of Plant Protecton, Chinese Academy of Agricultural Sciences, Beijing 100193, China	huangzhaofeng@caas.cn

Abstract:To elucidate the resistance mechanisms of tribenuron-methyl-resistant weed Silene conoidea population from wheat fields in Hebei Province, this study conducted whole-plant bioassays, target-site acetolactate synthase (ALS) gene sequencing analysis, ALS activity assays, metabolic enzyme inhibition experiments, and cross-resistance and multi-resistance determinations. The results showed that the GR₅₀ of the DZ-2R population to tribenuron-methyl was 527.60 g (a.i.)/hm², whereas that of the S2400 population was 3.99 g (a.i.)/hm², resulting in a resistance index of 132.23, which at a high resistance level. ALS gene sequencing revealed a point mutation at the nucleotide position 574 in the DZ-2R population, where the codon for tryptophan (TGG) was substituted by leucine (TTG), and there was no significant difference in the relative copy number and relative expression levels of the ALS gene between the two populations. ALS activity assays showed that the I₅₀ value of the DZ-2R population was 211.50 μmol/L, which was 19.71-fold higher than that of the S2400 population. In metabolic enzyme inhibition assays, pretreatment with the cytochrome P450 inhibitor malathion and the glutathione S-transferase inhibitor NBD-Cl reduced the GR₅₀ of the DZ-2R population by 53.43% and 60.48%, respectively. Cross- and multiple-resistance analyses indicated that the DZ-2R population exhibited cross-resistance to other ALS-inhibiting herbicides, including mesosulfuron-methyl, florasulam, and bispyribac-sodium, but remained sensitive to the bipyrazone and carfentrazone-ethyl. In conclusion, these results indicate that the resistance to tribenuron-methyl in the S. conoidea DZ-2R population is mediated by a combination of ALS target-site mutation and enhanced metabolic detoxification.

keywords:Silene conoidea tribenuron-methyl acetolactate synthase herbicide resistance resistance mechanism

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