大麦抗条纹病基因的定位分析 Click here to download the full text Citation：司二静,张宇,孟亚雄,李葆春,马小乐,王化俊.大麦抗条纹病基因的定位分析.Journal of Plant Protection,2019,46(4):723-729 Hits: Download times: Author Name Affiliation E-mail Si Erjing  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Zhang Yu  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China Gansu Plant Seed Administrative Station, Lanzhou 730020, Gansu Province, China    Meng Yaxiong  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Li Baochun  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Ma Xiaole  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Wang Huajun  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  whuajun@yahoo.com  中文摘要:为发掘大麦中抗条纹病的新基因，采用三明治法通过人工接种大麦条纹病菌Pyrenophora graminea强致病力菌株QWC对甘啤2号（免疫）与Alexis（高感）杂交F1代及F2代分离群体进行抗性遗传分析，利用群体分离分析法鉴定与抗病基因连锁的SSR标记，并通过QTL IciMapping软件构建遗传连锁图谱完成对抗病基因的定位。结果显示，甘啤2号与Alexis杂交F1代对大麦条纹病菌强致病力菌株QWC表现为免疫，F2代表现3∶1抗感分离，表明甘啤2号对菌株QWC的抗性由1个显性抗性基因控制，将该抗病基因暂命名为Rdg3；该基因位于大麦7H染色体上的SSR标记Bmag206和Bmag7之间，与二者的遗传距离分别为1.78 cM和2.86 cM。经与已定位于7H染色体上的抗病基因比较，发现Rdg3是一个新的抗条纹病基因，可作为大麦抗病育种的新种质资源。 中文关键词:大麦条纹病  分子标记  抗病基因  基因定位   Molecular mapping of a gene for resistance to barley leaf stripe Author Name Affiliation E-mail Si Erjing  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Zhang Yu  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China Gansu Plant Seed Administrative Station, Lanzhou 730020, Gansu Province, China    Meng Yaxiong  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Li Baochun  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Ma Xiaole  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Wang Huajun  Gansu Key Laboratory of Crop Important & Germplasm Enhancement, Gansu Provincial Key Laboratory of Aridland and Crop Science, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China College of Agronomy, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  whuajun@yahoo.com  Abstract:In order to identify new resistance genes to Pyrenophora graminea in barley, the resistance evaluation of F1 and F2 generations from the crosses between Ganpi 2 (immune) and Alexis (high susceptible), as well as the parents, were carried out with sandwich method. Based on bulk segregation analysis and microsatellite markers analysis, SSR marker linked with resistance genes was identified, and then QTL IciMapping software was used to calculate the genetic distance, construct genetic linkage map, and map resistance gene. The results of resistance evaluation showed that F1 population was immune, and the ratio of resistance/susceptible was 3:1 in F2 generation. This indicated that the barley leaf stripe resistance in Ganpi 2 was conferred by a single dominant gene, which was designated as Rdg3 temporarily. The resistance gene Rdg3 located on chromosome 7H was between SSR markers Bmag206 and Bmag7, and the genetic distance was 1.78 cM to Bmag206 and 2.86 cM to Bmag7. Furthermore, based on the comparison with resistance gene mapped on 7H, Rdg3 was a novel barley leaf stripe resistance gene, which might serve as a new germplasm in barley resistance breeding. keywords:barley leaf stripe  molecular marker  resistance gene  gene mapping View Full Text  View/Add Comment  Download reader

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