高寒草地青藏苔草拮抗内生细菌筛选、鉴定及其促生作用测定 Click here to download the full text Citation：金梦军,李珊珊,田文波,杨成德,王玉琴.高寒草地青藏苔草拮抗内生细菌筛选、鉴定及其促生作用测定.Journal of Plant Protection,2019,46(4):779-786 Hits: Download times: Author Name Affiliation E-mail Jin Mengjun  Laboratory for Biocontrol Engineering of Crop Diseases and Pests in Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Li Shanshan  Laboratory for Biocontrol Engineering of Crop Diseases and Pests in Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Tian Wenbo  Laboratory for Biocontrol Engineering of Crop Diseases and Pests in Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Yang Chengde  Laboratory for Biocontrol Engineering of Crop Diseases and Pests in Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  yangcd@gsau.edu.cn  Wang Yuqin  State Key Laboratory of Plateau Ecology and Agriculture, Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, Qinghai Province, China    中文摘要:为从青藏苔草Carex moorcroftii内生细菌中开发优势拮抗菌种资源，采用对峙培养法测定其叶片内生细菌对黄瓜枯萎病菌Fusarium oxysporum、马铃薯炭疽病菌Colletotrichum coccodes、孜然根腐病菌F. solani等9种病原真菌的拮抗作用，对所筛选的优良拮抗内生细菌进行形态学及16SrDNA和gyr-B基因序列分析鉴定，并将其经固体发酵制成菌剂，采用生防菌剂接种方法测定其对黄瓜和辣椒的促生作用。结果显示，从青藏苔草叶片中共分离得到14株内生细菌；以黄瓜枯萎病菌作为指示菌初筛得到8株具有拮抗作用的菌株，其中菌株1Y4、1Y5、和1Y7的抑菌率均为57.53%，菌株1Y11的抑菌率为58.44%，均高于平均抑菌率56.73%；菌株1Y4具有广谱抑菌活性，对孜然根腐病和马铃薯炭疽病菌的抑菌率分别达73.33%和71.43%，且具有固氮作用；结合形态学和分子生物学鉴定，确定菌株1Y4为西姆芽胞杆菌Bacillus siamensis；以0.5% 1Y4发酵菌剂处理黄瓜和辣椒，株高分别增加153.06%和117.33%，促生效果显著。表明青藏苔草内生细菌1Y4的防病促生作用良好，有开发为微生物菌剂的潜力。 中文关键词:高寒草地  青藏苔草  拮抗内生细菌  鉴定  促生   Screening, identification and detection of growth-promoting antagonistic endophytic bacteria from Carex moorcroftii in alpine grassland Author Name Affiliation E-mail Jin Mengjun  Laboratory for Biocontrol Engineering of Crop Diseases and Pests in Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Li Shanshan  Laboratory for Biocontrol Engineering of Crop Diseases and Pests in Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Tian Wenbo  Laboratory for Biocontrol Engineering of Crop Diseases and Pests in Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China    Yang Chengde  Laboratory for Biocontrol Engineering of Crop Diseases and Pests in Gansu Province, College of Plant Protection, Gansu Agricultural University, Lanzhou 730070, Gansu Province, China  yangcd@gsau.edu.cn  Wang Yuqin  State Key Laboratory of Plateau Ecology and Agriculture, Academy of Animal Science and Veterinary Medicine, Qinghai University, Xining 810016, Qinghai Province, China    Abstract:In order to exploit the resources of dominant bacteria with antagonistic function from the endophytic bacteria of Carex moorcroftii, the antagonistic effects of endophytic bacteria against nine fungi such as Fusarium oxysporum, Colletotrichum coccodes, F. solani, etc. were evaluated by using the flat confrontation method; the selected endophytic bacteria with excellent antagonism were identified based on morphology and sequence analyses of 16S rDNA and gyr-B, and the growth-promoting effects of endophytic bacteria on cucumbers and peppers treated with the bacterial fertilizer fermented with selected endophytic bacteria were determined. The results showed that 14 endophytic bacterial strains were isolated from the leaves of C. moorcroftii, and eight endophytic bacteria with antagonistic activity were obtained by preliminary screening of the strains against F. oxysporum. Among them, the inhibition rates of strains 1Y4, 1Y5 and 1Y7 were 57.53%, and the inhibition rate of strain 1Y11 were 58.44%, both higher than 56.73% (the average inhibition rate). The strain 1Y4 had broad-spectrum antibiotics, especially against F. solani. and C. coccodes, with their inhibition rates reaching to 73.33% and 71.43%, respectively; it also had the nitrogen-fixing function. According to morphological and sequence analyses, strain 1Y4 was identified as Bacillus siamensis. When the cucumbers and peppers were treated with 0.5% fermentation agents of strain 1Y4, the height of plants was increased by 153.06% and 117.33%, respectively. The results indicated that the endophytic bacterial strain 1Y4 from leaves of C. moorcroftii in alpine grasslands had great anti-fungal and growth-promoting effects, and it also had the great potential for the development of microbial agents. keywords:alpine grassland  Carex moorcroftii  antagonistic endophytic bacteria  identification  growth-promoting View Full Text  View/Add Comment  Download reader

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