四种非寄主植物精油对小菜蛾的生物活性 Click here to download the full text Citation：宋程飞,冯雪梦,王志宇,闫喜中,郝赤.四种非寄主植物精油对小菜蛾的生物活性.Journal of Plant Protection,2022,49(2):671-682 Hits: Download times: Author Name Affiliation E-mail SONG Cheng-fei  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    FENG Xue-meng  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    WANG Zhi-yu  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    YAN Xi-zhong  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  yanxizhong80@163.com  HAO Chi  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  sxauhc@163.com  中文摘要:为寻找小菜蛾Plutella xylostella绿色防控方法,于室内测定辣蓼、迷迭香、芸香和紫苏4种非寄主植物精油对小菜蛾的生物活性。结果表明,辣蓼植物精油对小菜蛾2龄幼虫的毒杀作用最强,24、48和72 h时的致死中浓度LC50分别为2.18、1.78和1.46μg/μL,芸香植物精油次之;当芸香植物精油浓度为15μg/μL时,小菜蛾3龄幼虫的选择拒食率最高,作用24 h和48 h时分别为100.00%和92.84%,而在10μg/μL浓度下作用24 h和48 h时非选择拒食率达到最高值,分别为65.72%和42.72%;在4种植物精油中,芸香植物精油对小菜蛾3龄幼虫的生长抑制作用最明显,相对生长率最低,为-34.72%,生长抑制率最高,为133.74%,辣蓼植物精油的生长抑制效果次之,迷迭香和紫苏植物精油的抑制作用相对较差;小菜蛾雌、雄成虫对4种植物精油均表现出一定的触角电位反应和驱避活性,其中,芸香植物精油的驱避效果最明显;气相色谱-质谱分析结果显示,芸香植物精油的主要成分为β-石竹烯、(+)-α-蒎烯和β-蒎烯。表明芸香植物精油对小菜蛾具有显著的生物活性,具备开发为小菜蛾靶标杀虫剂的潜力。 中文关键词:小菜蛾  芸香植物精油  生物活性   Bioactivities of the essential oils derived from four species of non-host plants against diamondback moth Plutella xylostella Author Name Affiliation E-mail SONG Cheng-fei  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    FENG Xue-meng  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    WANG Zhi-yu  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China    YAN Xi-zhong  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  yanxizhong80@163.com  HAO Chi  College of Plant Protection, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China  sxauhc@163.com  Abstract:In search for the green prevention and control method of diamondback moth Plutella xylostella, the biological activities of the essential oils from four non-host plants, Polygonum hydropiper,Rosmarinus officinalis, Ruta graveolens and Perilla frutescens, against P. xylostella were determined in the laboratory. The results showed that the toxicity of P. hydropiper plant essential oil against the 2nd-instar larvae was the strongest, with a median lethal concentration(LC50) value of 2.18, 1.78 and 1.46 μg/μL at 24, 48 and 72 h, respectively, followed by Ru. graveolens plant essential oil. The choice antifeedant rate of Ru. graveolens plant essential oil at 15 μg/μL against the 3rd-instar larvae was the highest, which was 100.00% and 92.84% at 24 h and 48 h, respectively. Its non-choice antifeedant rate reached the peak values at the concentration of 10 μg/μL, which was 65.72% and 42.72% at 24 h and 48 h. Ru. graveolens plant essential oil had the most obvious inhibitory effect on the growth of the 3rd-instar larvae among the four species of plant essential oils, resulting in a lowest relative growth rate of -34.72% and a highest growth inhibition rate of 133.74%, followed by P. hydropiper plant essential oil, and Ro. officinalis and P. frutescens plant essential oils exhibited a relatively poor inhibitory effect. The female and male adults of P. xylostella displayed certain electroantennogram(EAG) responses and repellent activities to the four plant essential oils. Ru. graveolens plant essential oil showed the most obvious repellent effect. The result of gas chromatography-mass spectrometer(GC-MS) assay showed that the main components of Ru. graveolens plant essential oil were β-caryophyllene,(+)-α-pinene and β-pinene. It indicated that Ru. graveolens plant essential oil had significant biological activity against P. xylostella, and had the potential to be developed as an insecticide for P. xylostella. keywords:Plutella xylostella  Ruta graveolens plant essential oil  bioactivity View Full Text  View/Add Comment  Download reader

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