| 制霉菌素对褐飞虱取食行为、营养及共生菌数量的影响 |
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| 引用本文：张珏锋,何月平,陈建明.制霉菌素对褐飞虱取食行为、营养及共生菌数量的影响.植物保护学报,2015,42(2):264-270 |
| DOI：10.13802/j.cnki.zwbhxb.2015.02.018 |
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| 中文摘要:为明确制霉菌素对褐飞虱取食行为、营养和共生菌数量的影响, 用150、300和600 U/mL制霉菌素处理的水稻饲养褐飞虱, 利用刺探电位图、高效液相色谱和镜检法监测其取食行为及体内必需氨基酸含量和类酵母共生菌(yeast-like symbiotes,YLS)数量。结果表明, 取食300、600 U/mL制霉菌素处理水稻的褐飞虱3龄若虫体内YLS数量显著低于未处理水稻(对照), 而150 U/mL处理与对照无显著差异;5龄若虫体内YLS数量随制霉菌素浓度的增加而显著降低, 但600 U/mL与300 U/mL处理间差异不显著。褐飞虱于韧皮部吸食波出现次数在对照中显著多于制霉菌素处理, 不同浓度间无明显差异;制霉菌素对褐飞虱的吸食时间影响不明显, 但对其体内必需氨基酸含量影响较大, 600 U/mL处理下均显著低于对照和其余处理, 300 U/mL处理下除丙氨酸、脯氨酸、蛋氨酸和酪氨酸外均显著低于对照, 150 U/mL处理下也均显著低于对照。表明制霉菌素能显著降低褐飞虱体内YLS数量和各种必需氨基酸含量, 但对其吸食时间的影响不明显。 |
| 中文关键词:褐飞虱 类酵母共生菌 制霉菌素 刺吸电位图 必需氨基酸 |
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| Effects of nystatin on the feeding behavior, nutrition and yeast-like symbiont number of the brown planthopper, Nilaparvata lugens (Stål) |
| Author Name | Affiliation | E-mail | | Zhang Juefeng | State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China | | | He Yueping | State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China
College of Plant Science & Technology of Huazhong Agricultural University, Wuhan 430070, Hubei Province, China | | | Chen Jianming | State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China | chenjm63@163.com |
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| Abstract:The effects of nystatin on the brown planthopper (BPH), Nilaparvata lugens, were determined by using electrical penetration graph (EPG), high performance liquid chromatography (HPLC) and microscopic examination. The BPHs were reared on the 0, 150, 300 or 600 U/mL concentrations of nystatin-treated rice plants, respectively. The results showed that the numbers of yeast-like symbiotes (YLS) in nystatin-treated 3rd instar BPH nymphs were lower than those of the control except the 150 U/mL treatment. The numbers of YLS in 5th instar BPH nymphs declined remarkably along with increasing nystatin concentration. But no significant difference was detected between 300 U/mL and 600 U/mL nystatin-treated populations. The frequency of N4-b (a parameter for determining the feeding behavior of BPH) was significantly higher in control population than that in all nystatin treated populations. However, no significant difference was detected among all nystatin treated concentrations. The nystatin had no effect on the feeding time of BPH, but it had a significant impact on the content of essential amino acids. The contents of all essential amino acids of BPH in 600 U/mL nystatin treatment were significant lower than those of BPH in other treatments; the contents of most essential amino acids of BPH in 300 U/mL nystatin treatment and the contents of all essential amino acids of BPH in 150 U/mL nystatin treatment were also significantly lower than those of BPH in the control treatment. In conclusion, nystatin could significantly reduce the number of YLS and the content of essential amino acids in BPH, but no remarkable effects on feeding behavior. |
| keywords:Nilaparvata lugens yeast-like symbiote nystatin feeding behavior essential amino acids |
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