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普通小麦-华山新麦草易位系9020-17-25-6抗条锈性遗传分析及分子标记

引用本文：马东方,尹军良,刘署艳,王文凯,方正武,井金学.普通小麦-华山新麦草易位系9020-17-25-6抗条锈性遗传分析及分子标记.植物保护学报,2015,42(3):327-333

DOI：10.13802/j.cnki.zwbhxb.2015.03.007

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作者	单位	E-mail
马东方	长江大学农学院, 湿地生态与农业利用教育部工程研究中心, 主要粮食作物产业化湖北省协同创新中心, 湖北荆州 434025 西北农林科技大学, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100
尹军良	西北农林科技大学, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100
刘署艳	湖北省荆州农业科学院, 荆州 434000
王文凯	西北农林科技大学, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100
方正武	长江大学农学院, 湿地生态与农业利用教育部工程研究中心, 主要粮食作物产业化湖北省协同创新中心, 湖北荆州 434025	fangzhengwu88@163.com
井金学	西北农林科技大学, 旱区作物逆境生物学国家重点实验室, 陕西杨凌 712100	jingjinxue@163.com

中文摘要:为明确普通小麦-华山新麦草易位系9020-17-25-6的抗条锈病基因及其遗传特点,利用中国条锈菌小种CYR29对9020-17-25-6、铭贤169及其杂交后代F₁、F₂、F₃代进行苗期抗条锈性鉴定及遗传分析,选取48条RGAP引物和491对SSR引物对接种CYR29的F₂代群体进行筛选,寻找与抗病基因连锁的分子标记.结果表明:9020-17-25-6对CYR29具有良好的抗条锈性,由1对显性基因独立控制,暂定名为YrHua9020.筛选到2个RGAP标记(M1和M2)和位于染色体3AS上的4个SSR标记(Xwmc11、Xwmc532、Xcfd79、Xgwm2)与YrHua9020连锁,与目的基因的遗传距离分别为6.9、9.5、17.8、12.2、7.2和17.8 cM.与已定位于3A 染色体上的抗条锈病基因的比较研究表明,YrHua9020 是一个与已知基因不同的新的抗条锈病基因.

中文关键词:小麦条锈病华山新麦草抗病基因遗传分析

Genetic and molecular mapping of stripe rust resistance gene in wheat-Psathyrostachys huashanica translocation line 9020-17-25-6

Author Name	Affiliation	E-mail
Ma Dongfang	College of Agriculture, Yangtze University Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education Hubei Collaborative Innovation Center for Grain Industry, Jingzhou 434025, Hubei Province, China State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Yin Junliang	State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Liu Shuyan	Jingzhou Academy of Agricultural Sciences of Hubei Province, Jingzhou 434000, Hubei Province, China
Wang Wenkai	State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi Province, China
Fang Zhengwu	College of Agriculture, Yangtze University Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education Hubei Collaborative Innovation Center for Grain Industry, Jingzhou 434025, Hubei Province, China	fangzhengwu88@163.com
Jing Jinxue	State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi Province, China	jingjinxue@163.com

Abstract:9020-17-25-6 is a translocation line derived from Triticum aestivum and Psathyrostachys huashanica through cross and back-cross. To study and locate the resistance genes to stripe rust in 9020-17-25-6, the seedlings of F₁, F₂ and F₃ derived from 9020-17-25-6 crossing with susceptible parent Mingxian 169 were tested in greenhouses for genetic analysis of stripe rust resistance. The resistance gene-analog polymorphism (RGAP) and simple sequence repeat (SSR) techniques were used to screen molecular marker linked to the gene conferring resistance to Puccinia striiformis f. sp. tritici race CYR29. The genetic results indicated that 9020-17-25-6 had a single dominant gene conferring resistance to CYR29, temporarily designated as YrHua9020. Two RGAP markers M1 and M2, and four SSR markers Xwmc11, Xwmc532, Xcfd79 and Xgwm2 were tightly linked to the resistance gene on wheat chromosome 3AS, with genetic distance of 6.9, 9.5, 17.8, 12.2, 7.2 and 17.8 cM, respectively. Furthermore, based on chromosomal location, reactions to different pathotypes and pedigree analysis, YrHua9020 was a new resistance gene to stripe rust.

keywords:stripe rust Psathyrostachys huashanica Keng stripe rust resistance gene genetic analysis

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