| 拮抗菌株xj063-1发酵条件的优化及室内防效测定 |
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| 引用本文：刘晓琳,马荣,梁英梅,韩文静,陈梦,王雪剑.拮抗菌株xj063-1发酵条件的优化及室内防效测定.植物保护学报,2015,42(5):820-826 |
| DOI：10.13802/j.cnki.zwbhxb.2015.05.018 |
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| 中文摘要:为探讨菌株xj063-1的最优发酵条件,以菌体生物量和对病原菌的抑制率为指标,采用单因素方法对菌株的发酵条件进行优化,并将优化的发酵液在离体枣果上进行防效测定。结果表明,当装液量为50 mL时,拮抗菌株生物量OD600为1.987,对病原菌的抑制率为67.1%;接种量为装液量体积的4%时,生物量OD600为1.573,对病原菌的抑制率为59.7%;初始pH 7.0时,其OD600为1.905,对病原菌的抑制率为65.6%;温度在30℃、转速180 r/min时,OD600 分别为1.969和1.982,对病原菌的抑制率分别为62.2%和61.2%。拮抗菌株优化后的发酵液通过预防法处理后的枣果的病斑面积为4 mm2,对照发病面积为121 mm2,抑制率为96.7%;通过治疗法处理后的枣果的病斑面积为12.25 mm2,抑制率为89.9%,表明菌株xj063-1具有较好的生防潜力。 |
| 中文关键词:枣果黑斑病 枯草芽胞杆菌 发酵条件优化 防效 |
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| Optimization and fermentation conditions of antagonistic strain xj063-1 and its effect on jujube black spot |
| Author Name | Affiliation | E-mail | | Liu Xiaolin | College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang Uygur Autonomous Region, China | | | Ma Rong | College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang Uygur Autonomous Region, China
Key Laboratory for Silviculture and Conservation of Ministry of Education, Beijing Forestry University, Beijing 100083, China | xjaumr@sina.com | | Liang Yingmei | Museum of Beijing Forestry University, Beijing 100083, China | | | Han Wenjing | College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang Uygur Autonomous Region, China | | | Chen Meng | Forest Pest Control and Quarantine Bureau of Xinjiang, Urumqi 830000, Xinjiang Uygur Autonomous Region, China | | | Wang Xuejian | College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang Uygur Autonomous Region, China | |
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| Abstract:In order to investigate best conditions for fermentation liquid of antagonistic strain xj063-1, the single factor experiments were performed with bacterial biomass and the inhibition rate of pathogenic bacteria as indicators. The results showed that the optimal fermentation conditions were composed of the following factors: liquid volume, 50 mL; inoculum, 4% (v/v); initial pH 7.0; fermentation temperature, 30℃; rotation speed, 180 r/min. Bacterial biomass OD600 and the inhibition rate of pathogenic bacteria were 1.987/67.1%, 1.573/59.7%, 1.905/65.6%, 1.969/62.2% and 1.982/61.2%, respectively. When optimized filtrate was treated on jujube fruits, the lesion diameters were 4 mm2 by prevention, and 12.25 mm2 by therapy, respectively. The control effects were 96.7% by prevention and 89.9% by therapy, respectively. These results indicated that the antagonistic strains may provide a new biological control method in controlling Alternaria alternate. |
| keywords:Alternaria alternate Bacillus subtilis fermentation condition optimization control effect |
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