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玉米抗南方锈病种质标记基因型鉴定与遗传多样性分析

引用本文：段灿星,江凯,秦子惠,孙素丽,宋凤景,王晓鸣.玉米抗南方锈病种质标记基因型鉴定与遗传多样性分析.植物保护学报,2015,42(6):899-907

DOI：10.13802/j.cnki.zwbhxb.2015.06.006

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作者	单位	E-mail
段灿星	中国农业科学院作物科学研究所, 国家农作物基因资源与基因改良重大科学工程, 北京 100081
江凯	中国农业科学院作物科学研究所, 国家农作物基因资源与基因改良重大科学工程, 北京 100081
秦子惠	中国农业科学院作物科学研究所, 国家农作物基因资源与基因改良重大科学工程, 北京 100081
孙素丽	中国农业科学院作物科学研究所, 国家农作物基因资源与基因改良重大科学工程, 北京 100081
宋凤景	中国农业科学院作物科学研究所, 国家农作物基因资源与基因改良重大科学工程, 北京 100081
王晓鸣	中国农业科学院作物科学研究所, 国家农作物基因资源与基因改良重大科学工程, 北京 100081	wangxiaoming@caas.cn

中文摘要:为阐明玉米抗南方锈病种质的标记基因型和遗传背景,利用7个与玉米抗南方锈病3个基因连锁的SSR标记鉴定了38份抗病玉米种质的标记基因型,并采用40个多态性SSR标记对39份抗南方锈病的玉米自交系和6个标准测验种进行了遗传多样性研究。结果表明,7个与抗病基因连锁的SSR标记将38份抗病种质鉴定为17种标记基因型,表明可能存在多样的抗性基因组合方式;辽2204等9份种质仅扩增出齐319的标记基因型,沈136和W456仅扩增出W2D的标记基因型;种质LO932未扩增出与齐319、P₂₅和W2D相同的标记,可能携带新的抗南方锈病基因;相近遗传背景的抗性种质分属不同的标记基因型,表明抗病种质可能携带的抗南方锈病基因在育种选择中发生了分离。40对多态性SSR引物在45份自交系中共检测出115个等位基因变异,平均每对引物检测到2.88个等位基因,变异范围为2~4;平均多态性信息含量为0.4649,变化范围为0.1258~0.6951;通过UPGMA聚类分析,39份抗病材料被划分到以标准测验种为代表的6个杂种优势亚群中,与系谱分析基本一致,这为在育种中合理利用抗源提供了信息。

中文关键词:玉米南方锈病 SSR标记抗病标记基因型遗传多样性

Identification of resistance marker genotype and genetic diversity of maize germplasm with resistance to southern corn rust

Author Name	Affiliation	E-mail
Duan Canxing	Institute of Crop Science/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jiang Kai	Institute of Crop Science/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Qin Zihui	Institute of Crop Science/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Sun Suli	Institute of Crop Science/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Song Fengjing	Institute of Crop Science/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Wang Xiaoming	Institute of Crop Science/National Key Facility of Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences, Beijing 100081, China	wangxiaoming@caas.cn

Abstract:To understand resistance marker genotype and genetic background of maize lines with resistance to southern corn rust (SCR), seven SSR markers linked to three SCR resistance genes were used to genotype 38 resistant maize inbred lines and 40 polymorphic SSR makers were adopted in genetic diversity analysis of 39 resistant maize lines. A total of 17 resistance marker genotypes were identified, indicating there may be different combinations of SCR resistance genes among 38 resistant maize accessions. The same marker genotypes as those of Qi 319 were amplified among nine lines, e.g. Liao 2204. The marker genotypes of Shen 136 and W456 lines were the same as those of W2D; LO932 might carry new SCR resistance gene which was different from Qi 319, P₂₅ and W2D lines. The lines with similar genetic background belonged to different marker genotypes, suggesting that new combination or separation of resistance genes occurred in the course of breeding. A total of 115 alleles were detected in 45 maize lines with an average of 2.88 and a range of 2-4 per locus. The polymorphism information content (PIC) averaged 0.4649, ranging from 0.1258 to 0.6951. Using UPGMA analysis, the 45 maize lines were classified into six heterosis subgroups, which were consistent with pedigree information and would provide basis for utilizing resistance germplasm in breeding.

keywords:maize southern corn rust SSR marker resistance marker genotype genetic diversity

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