| 参与烟粉虱免疫反应的clip丝氨酸蛋白酶基因分析 |
| 点此下载全文 |
| 引用本文：于洁,王登杰,张林雅,雷仲仁,王海鸿.参与烟粉虱免疫反应的clip丝氨酸蛋白酶基因分析.植物保护学报,2016,43(1):55-61 |
| DOI：10.13802/j.cnki.zwbhxb.2016.01.008 |
| 摘要点击次数: |
| 全文下载次数: |
| 作者 | 单位 | E-mail | | 于洁 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | | 王登杰 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | | 张林雅 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | | 雷仲仁 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | | 王海鸿 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | wanghaihong2020@sina.com |
|
|
| 中文摘要:为了解参与烟粉虱免疫反应的clip丝氨酸蛋白酶(clip-domain serine proteases in Bemisia tabaci,BtCLIPs)基因,对已鉴定的8个BtCLIPs基因编码的蛋白进行序列分析,与冈比亚按蚊、家蚕、黑腹果蝇、烟草天蛾4种模式昆虫的同源蛋白进行系统进化分析,并通过qRT-PCR检测球孢白僵菌侵染烟粉虱4龄若虫后BtCLIPs的时间表达模式.结果显示,8个BtCLIPs基因均为含有CLIPs结构域的丝氨酸蛋白酶基因;BtCLIP19、BtCLIP20和BtCLIP21独自聚在进化树的一支上;其它BtCLIPs均与4种模式昆虫中的1种或多种同源蛋白聚在一起;与对照相比,8个BtCLIPs基因受球孢白僵菌侵染后在不同时间的相对表达量均上调,但诱导程度不同,48 h的相对表达量达最高,其中BtCLIP19上调表达最明显,为对照组的71倍.研究表明,8个BtCLIPs可能参与烟粉虱对真菌侵染的免疫反应,并成为烟粉虱生物防治的新靶标. |
| 中文关键词:烟粉虱 丝氨酸蛋白酶 clip结构域 免疫反应 |
| |
| Analysis on clip serine protease genes involved in innate immunity of Bemisia tabaci (Hemiptera: Aleyrodidae) |
| Author Name | Affiliation | E-mail | | Yu Jie | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | | Wang Dengjie | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | | Zhang Linya | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | | Lei Zhongren | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | | Wang Haihong | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | wanghaihong2020@sina.com |
|
|
| Abstract:To study clip-domain serine proteases in tobacco whitefly Bemisia tabaci, eight CLIP serine proteases identified in B. tabaci were analyzed by protein sequence alignment and phylogenetic analysis with homologous CLIPs in Anopheles gambiae, Bombyx mori, Drosophila melanogaster and Manduca sexta. The 4th instar B. tabaci nymphs were infected by Beauveria bassiana and the expression of clips serine protease genes was subsequently analyzed by qRT-PCR. The results showed that the eight BtCLIPs genes contained CLIP domain; BtCLIP19, BtCLIP20 and BtCLIP21 formed a specific clade. The other BtCLIPs were evolutionarily close to one or more homologous serine proteases in the four model insects. The transcriptions of eight BtCLIPs genes in B. tabaci infected by B. bassiana were up-regulated and peaked at 48 h. The transcription of BtCLIP19 was the highest among all BtCLIPs genes and increased 71 folds compared to the control. The eight BtCLIPs genes probably participated in the innate immune responses to the fungus infection, and could be a new target for controlling B. tabaci. |
| keywords:Bemisia tabaci serine protease clip domain immunological reaction |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 14644255 版权所有：植物保护学报 京ICP备05006550号-2 主管单位：中国科协 主办单位：中国植物保护学会、中国农业大学 地址：北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话：010-62732528 电子邮件：zbxb@cau.edu.cn 技术支持： 北京勤云科技发展有限公司 |
