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我国部分地区常见农作物上黄瓜花叶病毒分离物核酸多样性分析

引用本文：陈玉珍,谭新球,朱春晖,孙书娥,刘勇,张德咏.我国部分地区常见农作物上黄瓜花叶病毒分离物核酸多样性分析.植物保护学报,2016,43(3):427-433

DOI：10.13802/j.cnki.zwbhxb.2016.03.011

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作者	单位	E-mail
陈玉珍	湖南农业大学植物保护学院, 长沙 410128 湖南省农业科学院植物保护研究所, 园艺作物害虫治理湖南省重点实验, 长沙 410125
谭新球	湖南省农业科学院植物保护研究所, 园艺作物害虫治理湖南省重点实验, 长沙 410125
朱春晖	湖南省农业科学院植物保护研究所, 园艺作物害虫治理湖南省重点实验, 长沙 410125
孙书娥	湖南省农业科学院植物保护研究所, 园艺作物害虫治理湖南省重点实验, 长沙 410125
刘勇	湖南农业大学植物保护学院, 长沙 410128 湖南省农业科学院植物保护研究所, 园艺作物害虫治理湖南省重点实验, 长沙 410125
张德咏	湖南农业大学植物保护学院, 长沙 410128 湖南省农业科学院植物保护研究所, 园艺作物害虫治理湖南省重点实验, 长沙 410125	dyzhang78@gmail.com

中文摘要:为明确我国黄瓜花叶病毒株系分化及系统进化基本情况,从湖南、新疆、青海和海南4省区采集1367个样品对其进行酶联免疫和RT-PCR检测,并对分离获得的15个黄瓜花叶病毒(Cucumber mosaic virus,CMV)纯化分离物CP、MP、2b核苷酸序列进行相似性和进化树分析及生物学性状比较。结果表明,辣椒、龙葵和黄瓜的CMV阳性检出率较高,分别为54.13%、29.19%和18.46%。进化树分析显示CMV-Q5与CMV亚组II的亲缘性较高;CMV-N7为新发现的重组株系,其CP、2b基因属于CMV亚组IB,MP基因却属于CMV亚组II;其余13个分离物均属于CMV亚组IB。CMV-N7和CMV-Q5在系统寄主心叶烟和枯斑寄主苋色藜上引发的症状相似,但比对照株系CMV-P3613(IB)的发病时间要晚1~2 d,系统花叶较温和,枯斑较小。表明在以上4省区常见农作物上广泛流行的CMV存在分子变异。

中文关键词:黄瓜花叶病毒检测进化核酸多样性

Sequence diversity analysis of Cucumber mosaic virus isolates from common crops in China

Author Name	Affiliation	E-mail
Chen Yuzhen	College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China
Tan Xinqiu	Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China
Zhu Chunhui	Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China
Sun Shu'e	Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China
Liu Yong	College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China
Zhang Deyong	College of Plant Protection, Hunan Agricultural University, Changsha 410128, Hunan Province, China Key Laboratory of Integrated Management of Pests and Diseases on Horticultural Crops in Hunan Province Institute of Plant Protection, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China	dyzhang78@gmail.com

Abstract:In order to explore the strain differentiation and phylogenetic characteristics of Cucumber mosaic virus(CMV)in China, 1367 samples displaying relevant symptoms from Hunan, Xinjiang, Qinghai and Hainan were detected by using enzyme-linked immunosorbent assay (ELISA) and reverse transcription-polymerase chain reaction (RT-PCR) methods. The results showed that the CMV detection rates of chili, Chenopodium amaranticolor and cucumber plants were 54.13%, 29.19% and 18.46%, respectively. Moreover, 15 isolates of CMV were obtained through biological purification. The phylogenetic analysis based on nucleotide sequences of 2b, CP and MP showed that CMV-Q5 was classified as CMV subgroup II; CMV-N7 was a new natural reassortant between CMV subgroups, with its CP and 2b being derived from CMV subgroup IB, while MP was derived from CMV subgroup II. Other 13 isolates were classified as CMV subgroup IB. Compared with CMV-P3613(IB) standard strain, CMV-N7 and CMV-Q5 were significantly different in biological characteristics as their infection symptoms were milder and appeared two days later on Nicotiana glutinosa and Chenopodium amaranticolor. These results indicated that the CMV isolates infecting common crops in the four regions presented some molecular variation.

keywords:Cucumber mosaic virus detection evolution sequence diversity

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