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橡胶树白粉菌Oidium heveae侵染寄主拟南芥的筛选

引用本文：梁羽萍,何其光,刘文波,林春花,缪卫国,郑服丛.橡胶树白粉菌Oidium heveae侵染寄主拟南芥的筛选.植物保护学报,2016,43(4):567-572

DOI：10.13802/j.cnki.zwbhxb.2016.04.006

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作者	单位	E-mail
梁羽萍	海南大学环境与植物保护学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228
何其光	海南大学环境与植物保护学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228
刘文波	海南大学环境与植物保护学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228
林春花	海南大学环境与植物保护学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228
缪卫国	海南大学环境与植物保护学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228	weiguomiao1105@126.com
郑服丛	海南大学环境与植物保护学院, 海南省热带生物资源可持续利用重点实验室, 海口 570228	zhengfucong@126.com

中文摘要:为筛选出橡胶树白粉菌Oidium heveae Steimnann新的寄主载体，通过接种野生型拟南芥Col-0、突变体Sr1-4D及eds1，观察了橡胶树白粉菌侵染不同拟南芥突变体的过程，PCR扩增测序法验证相关致病基因，构建橡胶树白粉菌-拟南芥互作体系。结果表明，在Col-0、Sr1-4D叶片上面，橡胶树白粉菌产生部分菌丝后停止生长，不能形成典型的橡胶树白粉病症状；但能成功侵染eds1叶片，在叶片的正、背面有银白色辐射状菌丝，后期在病斑上出现一层粉层，表现橡胶树白粉病的典型症状。组织染色和显微观察结果显示，在突变体eds1叶片上橡胶树白粉菌完成了侵染过程。PCR扩增测序结果表明接种后突变体eds1叶片上及组织中病菌均为橡胶树白粉菌；使用橡胶树白粉菌3个致病相关基因作为靶标，验证了eds1和橡胶树上白粉菌基因组中的3个致病相关基因相似度均达到99%～100%。表明橡胶树白粉菌可侵染拟南芥突变体eds1。

中文关键词:橡胶白粉菌拟南芥侵染 eds1

Screening of Arabidopsis thaliana infected by rubber tree powdery mildew Oidium heveae

Author Name	Affiliation	E-mail
Liang Yuping	Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Environmental and Plant Protection, Hainan University, Haikou 570228, Hainan Province, China
He Qiguang	Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Environmental and Plant Protection, Hainan University, Haikou 570228, Hainan Province, China
Liu Wenbo	Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Environmental and Plant Protection, Hainan University, Haikou 570228, Hainan Province, China
Lin Chunhua	Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Environmental and Plant Protection, Hainan University, Haikou 570228, Hainan Province, China
Miao Weiguo	Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Environmental and Plant Protection, Hainan University, Haikou 570228, Hainan Province, China	weiguomiao1105@126.com
Zheng Fucong	Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources, College of Environmental and Plant Protection, Hainan University, Haikou 570228, Hainan Province, China	zhengfucong@126.com

Abstract:To screen successful Arabidopsis thaliana mutants as a host of rubber tree powdery mildew pathogen and confirm Oidium heveae Steimnann reproduction in A.thaliana mutants eds1, which would take the place of rubber-O. heveae interaction system, A.thaliana type Col-0, mutant Sr1-4D and mutant eds1 were inoculated for the infection of O.heveae. The results showed that, by brushing inoculation method, O. heveae produced some hyphae and then stopped growing on Col-0 and Sr1-4D leaves, and the pathogen infection was not able to form the typical symptoms of rubber tree powdery mildew on the leaf of Col-0, Sr1-4D. The pathogen was able to successfully infect the leaves from eds1, and silvery white radial hyphae were able to be observed on the two sides of the leaf, lately the powder layer appeared on the diseased spots of the leaves, which was the same as the typical symptom of rubber tree powdery mildew. Combined further tissue staining and microscopic observation, the rubber tree powdery mildew pathogen was able to finish the infection process in the mutant eds1 leaf. On the molecular level, ITS sequence amplification and sequencing results further confirmed that the pathogen was O. heveae. The similarity of three pathogenicity related genes, which were from O. heveae infected A. thaliana eds1 and rubber tree Reyan 7-33-97 clone respectively, had reached to 99%-100% after PCR amplification with the corresponding primers and sequencing. The results indicated that rubber tree O. heveae was able to infect A. thaliana mutant eds1.

keywords:Oidium heveae Arabidopsis thaliana infection eds1

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