| 人参灰霉病拮抗细菌的筛选及鉴定 |
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| 引用本文：孙卓,杨利民.人参灰霉病拮抗细菌的筛选及鉴定.植物保护学报,2016,43(6):935-942 |
| DOI：10.13802/j.cnki.zwbhxb.2016.06.008 |
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| 中文摘要:为获取对灰葡萄孢Botrytis cinerea Pers. ex Fr.防效优良的菌株，采用滤纸片法和牛津杯法从105株菌株中筛选出7株拮抗性良好的菌株，采用平板对峙法对抑菌效果较好的菌株SZ-35进行抑菌谱测定，采用盆栽法研究其对人参灰霉病的防效及在人参体内的定殖能力，并通过形态学、生理生化特征、16S rDNA及gyrB基因测序确立其分类地位。结果显示，菌株SZ-35对灰葡萄孢的抑制作用最强，抑菌率和抑菌带宽分别为86.53%和12.20 mm，且具有广谱抑菌能力；菌株SZ-35可有效定殖于人参体内，对人参灰霉病的防效达76.42%，显著高于70%代森锰锌可湿性粉剂；通过形态特征、生理生化特征观察，以及16S rDNA及gyrB同源性序列对比分析，鉴定菌株SZ-35为解淀粉芽胞杆菌Bacillus amyloliquefaciens（GenBank登录号为KC511113）。研究表明，解淀粉芽胞杆菌SZ-35对灰葡萄孢具有良好的抑菌能力，且在人参中的定殖能力较好。 |
| 中文关键词:人参 拮抗细菌 定殖 生物防治 |
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| Isolation and characterization of antagonistic bacteria against ginseng grey mould |
| Author Name | Affiliation | E-mail | | Sun Zhuo | Breeding Base of State Key Laboratory of JLP-MOST for Ecological Restoration and Ecosystem Management, College of Herbal Medicine, Jilin Agricultural University, Changchun 130118, Jilin Province, China | | | Yang Limin | Breeding Base of State Key Laboratory of JLP-MOST for Ecological Restoration and Ecosystem Management, College of Herbal Medicine, Jilin Agricultural University, Changchun 130118, Jilin Province, China | ylmh777@126.com |
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| Abstract:To obtain more effective antagonistic bacteria against Botrytis cinerea Pers. ex Fr., seven antagonistic bacteria strains were screened from 105 bacterial strains isolated from ginseng rhizosphere soils by using the Kirby-Bauer disk diffusion and the assay by Oxford cup plate. The antibacterial spectrum of strain SZ-35 was examined using flat-stand method. The colonization ability and control efficiency of strain SZ-35 against pray mold of ginseng were evaluated by pot experiment, and also the taxonomic status of strain SZ-35 was identified using morphology, physiological and biochemical characteristics, 16S rDNA and gyrB sequences. The results showed that the inhibitory effect of strain SZ-35 against B.cinerea was the highest among all strains and had broad-spectrum antibacterial function. The inhibition rate and inhibition zone were 86.53% and 12.20 mm. The strain SZ-35 could colony effectively in the root, stem and leaves of ginseng. The control efficacy of strain SZ-35 against pray mold of ginseng with 76.42% was higher than that of 70% mancozeb WP fungicide treatment. According to the morphology, physiological and biochemical characteristics, 16S rDNA and gyrB sequences, strain SZ-35 was identified as Bacillus amyloliquefaciens (GenBank accession no. KC511113). The results indicated that strain SZ-35 could successfully colony in the ginseng and effectively control B. cinerea. |
| keywords:ginseng antagonistic bacteria colonization biological control |
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