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Q型烟粉虱成虫传播番茄褪绿病毒的能力

引用本文：李娇娇,于毅,张秀霞,竺晓平,刘永光,张安盛.Q型烟粉虱成虫传播番茄褪绿病毒的能力.植物保护学报,2018,45(2):228-234

DOI：10.13802/j.cnki.zwbhxb.2018.2016063

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作者	单位	E-mail
李娇娇	山东省农业科学院植物保护研究所, 山东省植物病毒学重点实验室, 济南 250100
于毅	山东省农业科学院植物保护研究所, 山东省植物病毒学重点实验室, 济南 250100
张秀霞	山东省农业科学院植物保护研究所, 山东省植物病毒学重点实验室, 济南 250100
竺晓平	山东农业大学植物保护学院, 泰安 271018
刘永光	潍坊科技学院, 山东寿光 262700
张安盛	山东省农业科学院植物保护研究所, 山东省植物病毒学重点实验室, 济南 250100	zhangansheng2003@163.com

中文摘要:为明确Q型烟粉虱Bemisia tabaci成虫对番茄褪绿病毒（Tomato chlorosis virus，ToCV）的传毒特征，应用RT-PCR技术检测了ToCV在Q型烟粉虱成虫体内的存留时间、Q型烟粉虱成虫对ToCV的获毒效率及传毒效率。结果表明，Q型烟粉虱成虫离开带毒番茄植株后，其带毒率随时间的延长逐渐降低，最初为85.73%，96 h后仅为17.14%。在相同温度下，Q型烟粉虱成虫的获毒率先随着取食时间的延长而提高，而后处于相对稳定状态，当温度为21、26、31℃时，Q型烟粉虱成虫获毒率达到峰值的时间分别为72、48、6 h，最高获毒率分别为56.52%、54.17%和61.64%。当温度为26℃时，Q型烟粉虱成虫的传毒效率随着取食时间的延长而提高，取食时间为0.5 h时，番茄植株带毒率为25.00%，取食时间为48 h时，番茄植株带毒率达91.67%；温度对Q型烟粉虱成虫的传毒效率有极显著影响，取食48 h后，在21~31℃条件下番茄植株带毒率为83.33%~91.67%，在16℃条件下仅为16.67%。表明Q型烟粉虱成虫对ToCV具有较强的获毒和传毒能力，对其进行有效控制可以防止ToCV的扩散和蔓延。

中文关键词:番茄褪绿病毒 Q型烟粉虱传毒能力

Transmission of Tomato chlorosis virus (ToCV) by Bemisia tabaci biotype Q adults

Author Name	Affiliation	E-mail
Li Jiaojiao	Shandong Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Yu Yi	Shandong Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Zhang Xiuxia	Shandong Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China
Zhu Xiaoping	College of Plant Protection, Shandong Agricultural University, Tai'an 271018, Shandong Province, China
Liu Yongguang	Weifang University of Science and Technology, Shouguang 262700, Shandong Province, China
Zhang Ansheng	Shandong Key Laboratory of Plant Virology, Institute of Plant Protection, Shandong Academy of Agricultural Sciences, Jinan 250100, Shandong Province, China	zhangansheng2003@163.com

Abstract:In order to clarify the transmission characteristics of Tomato chlorosis virus (ToCV) by Bemisia tabaci biotype Q adults, the residence time of ToCV in B. tabaci biotype Q adults, the virus acquisition efficiency and transmission efficiency of B. tabaci biotype Q adults were studied with RT-PCR detection. The rate of B. tabaci biotype Q adult carrying virus reduced gradually with the time after they left the viruliferous tomato plant, which was 85.73% at first and 17.14% after 96 h. At the same temperature, the rate of B. tabaci biotype Q adult acquiring virus increased with the feeding time first and then inclined to be steady. When the experiment temperature were 21, 26 and 31℃, the times that the virus acquisition rate of B. tabaci biotype Q adults reached the peak were 72 h, 48 h and 6 h, respectively, and the highest virus acquisition rate were 56.52%, 54.17% and 61.64%, respectively. At 26℃ , the virus transmission efficiency of B. tabaci biotype Q adult increased with the feeding time. The viruliferous percent of tomato plant was 25.00% when the feeding time was 0.5 h, and it was 91.67% when the feeding time was 48 h. Temperature had significant influence on the virus transmission efficiency of B. tabaci biotype Q adult; when the feeding time was 48 h, the viruliferous percent of tomato plant was 83.33%-91.67% when the temperature was 21-31℃, but it was only 16.67% when the temperature was 16℃. Therefore, B. tabaci biotype Q adult had stronger acquisition efficiency and transmission efficiency for ToCV and should be effectively controlled to prevent the diffusion and spread of ToCV disease.

keywords:Tomato chlorosis virus Bemisia tabaci biotype Q transmitting ability

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