| 喜旱莲子草次生代谢物对莲草直胸跳甲谷胱甘肽S-转移酶和淀粉酶活性的影响 |
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| 引用本文：杨永昌,柴艳萍,马瑞燕,李鑫,郭艳琼,高玲玲.喜旱莲子草次生代谢物对莲草直胸跳甲谷胱甘肽S-转移酶和淀粉酶活性的影响.植物保护学报,2018,45(4):739-744 |
| DOI：10.13802/j.cnki.zwbhxb.2018.2017213 |
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| 中文摘要:为明确喜旱莲子草的次生代谢物对其专食性天敌-莲草直胸跳甲Agasicles hygrophila成虫解毒酶和消化酶的影响，利用不同浓度的橙花叔醇、齐墩果酸和甜菜碱浸叶处理喜旱莲子草Alternanthera philoxeroides，连续饲喂莲草直胸跳甲1~3 d后，测定莲草直胸跳甲谷胱甘肽S-转移酶（GSTs）和淀粉酶（AMS）的活性变化。结果表明，橙花叔醇、齐墩果酸和甜菜碱均能抑制莲草直胸跳甲的GSTs活性，0.1%橙花叔醇和20%甜菜碱处理24 h时的GSTs活性最低，分别为45.67 U/mg和53.95 U/mg，0.2%齐墩果处理72 h时的GSTs活性最低，为98.77 U/mg，且莲草直胸跳甲对橙花叔醇的刺激存在着一种适应机制；0.5%橙花叔醇和0.1%齐墩果酸处理24 h后的GSTs活性最高，分别为243.10 U/mg和250.22 U/mg，是对照的1.55倍和1.59倍，存在诱导激活现象。甜菜碱对莲草直胸跳甲的AMS活性有明显的抑制作用，20%甜菜碱处理72 h后AMS活性最低，为1.01 U/mg，是对照的55.92%。表明喜旱莲子草3种次生代谢物均能抑制谷胱甘肽S-转移酶活性，其中橙花叔醇和齐墩果酸对谷胱甘肽S-转移酶有诱导激活作用，而甜菜碱对淀粉酶抑制作用较强。 |
| 中文关键词:喜旱莲子草 莲草直胸跳甲 谷胱甘肽S-转移酶 淀粉酶 |
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| Effects of secondary metabolites of alligator weed Alternanthera philoxeroides on the enzyme activity of amylases and glutathione S-transferases in the adults of alligator weed flea beetle Agasicles hygrophila |
| Author Name | Affiliation | E-mail | | Yang Yongchang | College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Chai Yanping | College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Ma Ruiyan | College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Li Xin | College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | | | Guo Yanqiong | College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | guoyq1979@163.com | | Gao Lingling | College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi Province, China | |
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| Abstract:To study the effects of secondary metabolites of alligator weed Alternanthera philoxeroides on the detoxifying and digestive enzymes of Agasicles hygrophila, an introduced natural enemy for this invasive weed in China, the alligator weed was first dipped by various concentrations of secondary metabolites, nerolidol, oleanolic acid and betaine. After the flea beetles were fed with the treated alligator weed for 1-3 days. The activities of amylases (AMS) and glutathione S-transferases (GSTs) of A. hygrophila were analyzed. The results showed that nerolidol, oleanolic acid and betaine remarkably inhibited the GSTs activities with the lowest activities (45.67, 98.77 and 53.95 U/mg, respectively) at 24, 72 and 24 h, respectively. However, A. hygrophila could quickly adapt to nerolidol and oleanolic acid. The GSTs activities in A. hygrophila were highest (243.10 U/mg and 250.22 U/mg) among all treatments 24 h after treatment with nerolidol and oleanolic acid, which were 1.55 and 1.59 times of that of the control, respectively. The betaine had an inhibitory effect on AMS. The activity of amylases in A. hygrophila treated with 20% betaine was the lowest (1.01 U/mg) at 72 h, which was 55.92% of the control. The results indicated that all three secondary metabolites had a negative effect on GSTs in the early time; nerolidol and oleanolic acid activated the GSTs activity after a short period, and the amylases activity was adversely affected by betaine. |
| keywords:Alternanthera philoxeroides Agasicles hygrophila glutathione S-transferases amylase |
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