| 茉莉酸甲酯对辣椒抗青枯病的诱导效应及抗氧化酶活性的影响 |
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| 引用本文：赵显阳,盘柳依,陈明,付永琦,向妙莲,曾晓春.茉莉酸甲酯对辣椒抗青枯病的诱导效应及抗氧化酶活性的影响.植物保护学报,2018,45(5):1103-1111 |
| DOI：10.13802/j.cnki.zwbhxb.2018.2018128 |
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| 中文摘要:为探索茉莉酸甲酯（methyl jasmonate，MeJA）诱导辣椒抗青枯病的效应及与抗氧化酶活性的关系，以感青枯病品种粤红1号和抗青枯病品种辛香8号辣椒为试验材料，分别用浓度为0.025、0.05、0.1、0.5、1.0 mmol/L的MeJA浸种12 h和喷雾48 h处理后，接种浓度为107 CFU/mL的青枯病菌Ralstonia solanacearum，测定MeJA对青枯病菌的抑制活性以及辣椒幼苗的病情指数、抗氧化酶活性和丙二醛含量。结果表明，0.025~1.0 mmol/L MeJA对青枯病菌无抑制作用；MeJA浸种处理对粤红1号和辛香8号辣椒幼苗的诱导效果最高，分别为21.38%和25.17%，而喷雾处理的诱导效果可依次提高至31.64%和37.30%，且诱导效果随MeJA浓度先升高后降低。与对照相比，MeJA处理后2个品种辣椒幼苗的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、过氧化物酶（POD）、抗坏血酸过氧化物酶（APX）及谷胱甘肽还原酶（GR）活性均随MeJA浓度先升高后降低，而丙二醛（MDA）含量则先降低后升高；CAT、POD、APX和GR活性最大值均为抗病品种辛香8号经MeJA喷雾处理后所达峰值，依次为13.51、49.27、11.58和5.28 U/g FW，MDA含量最低值为13.58 μmol/g FW，而SOD活性最大值为感病品种粤红1号经MeJA喷雾处理所达峰值，为364.31 U/g FW。表明MeJA预处理可有效诱导辣椒抗青枯病，其诱导效应与其激活幼苗叶片抗氧化酶活性和降低丙二醛含量有关。 |
| 中文关键词:茉莉酸甲酯 辣椒青枯病 诱导抗性 抗氧化酶 |
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| Inductive effect of methyl jasmonate to bacterial wilt and the effects on the activities of antioxidant enzymes in pepper seedlings |
| Author Name | Affiliation | E-mail | | Zhao Xianyang | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Pan Liuyi | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Chen Ming | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Fu Yongqi | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Xiang Miaolian | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | mlxiang2010@126.com | | Zeng Xiaochun | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China
College of Life Science and Environmental Resources, Yichun University, Yichun 336000, Jiangxi Province, China | xchzeng2013@163.com |
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| Abstract:In order to explore the relationships between the inductive effect of methyl jasmonate (MeJA) on pepper seedlings against bacterial wilt and the activities of antioxidant enzymes, the susceptible variety Yuehong 1 and resistant variety Xinxiang 8 were used as the experimental materials and treated with 0.025, 0.05, 0.1, 0.5, 1.0 mmol/L MeJA by 12-hour seed-soaking and 48-hour leaf-spraying respectively, followed by inoculation with 107 CFU/mL Ralstonia solanacearum, and the inhibition effect of MeJA to R. solanacearum and the incidence of bacterial wilt as well as the activities of antioxidant enzymes and MDA content were determined. The results showed that there was no inhibition effect of 0.025-1.0 mmol/L MeJA against R. solanacearum, but the highest inductive effect of MeJA by soaking seeds was increased by 21.38% and 25.17% in the susceptible variety Yuehong 1 and resistant variety Xinxiang 8, respectively, and the best inductive effect of MeJA by spraying seedlings was 31.64% and 37.30%, respectively, higher than those of the control. The inductive effect increased first and then decreased with the treatment concentration of MeJA. Meanwhile, the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and glutathione reductase (GR) decreased first and then increased with the treatment concentration of MeJA in two pepper seedlings compared with the control, while the malondialdehyde (MDA) content decreased first and then increased. The maximum activities of CAT, POD, APX and GR were 13.51, 49.27, 11.58 and 5.28 U/g FW, respectively, which peaked in resistant variety Xinxiang 8 sprayed by MeJA, and the lowest MDA content was 13.58 μmol/g FW, but the highest SOD activity was 364.31 U/g FW in susceptible variety Yuehong 1 sprayed with MeJA. In summary, MeJA pretreatment could effectively induce resistance to bacterial wilt in pepper seedlings, and the inductive effect of MeJA might be related to the enhancement of antioxidant enzyme activity and the decrease of MDA content. |
| keywords:methyl jasmonate pepper bacterial wilt induced resistance antioxidant enzyme |
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