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植物源羊脂酸除草活性及其响应机制

引用本文：李祖任,黄勤勤,彭琼,周勇,周小毛,柏连阳.植物源羊脂酸除草活性及其响应机制.植物保护学报,2018,45(5):1161-1167

DOI：10.13802/j.cnki.zwbhxb.2018.2017056

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作者	单位	E-mail
李祖任	湖南省农业科学院, 湖南省农业生物技术研究所, 杂草生物学及安全防控湖南省重点实验室, 长沙 410125 湖南省农田杂草防控技术与应用协同创新中心, 娄底 417000
黄勤勤	湖南省农业科学院, 湖南省农业生物技术研究所, 杂草生物学及安全防控湖南省重点实验室, 长沙 410125
彭琼	湖南省农业科学院, 湖南省农业生物技术研究所, 杂草生物学及安全防控湖南省重点实验室, 长沙 410125 湖南省农田杂草防控技术与应用协同创新中心, 娄底 417000
周勇	湖南省农业科学院, 湖南省农业生物技术研究所, 杂草生物学及安全防控湖南省重点实验室, 长沙 410125
周小毛	湖南省农业科学院, 湖南省农业生物技术研究所, 杂草生物学及安全防控湖南省重点实验室, 长沙 410125 湖南省农田杂草防控技术与应用协同创新中心, 娄底 417000
柏连阳	湖南省农业科学院, 湖南省农业生物技术研究所, 杂草生物学及安全防控湖南省重点实验室, 长沙 410125 湖南省农田杂草防控技术与应用协同创新中心, 娄底 417000	lybai196712@163.com

中文摘要:为研制新型植物源除草剂，需从植物中筛选具有除草活性的天然产物，本研究将椰子经物理压榨成椰子油，椰子油经皂化、酸化、蒸馏分段，再经气质联用仪鉴定其中具有除草活性的产物，并采用室内生测法和田间药效试验对该产物的除草活性进行评价，测定其在不同光照和浓度条件下对小飞蓬Conyza canadensis的3种防御酶——L-苯丙氨酸解氨酶、多酚氧化酶和过氧化物酶活性的影响。结果表明：从椰子油中筛选的具有除草活性的产物经鉴定为羊脂酸，气质色谱图中保留时间为6.20 min。羊脂酸具有较高的除草活性，EC₅₀为14.07 mg/L，100 mL的20%羊脂酸水乳剂喷施后15 d对20 m²非耕地杂草的株防效和鲜重防效分别为90.25%和90.37%，即与对照药剂草甘膦异丙胺盐水剂除草效果相当。低浓度羊脂酸对小飞蓬的防御酶活性影响不显著，较高浓度下L-苯丙氨酸解氨酶活性显著上升，多酚氧化酶和过氧化物酶活性显著下降，当光强为100~120 μmol·m^-2·s^-1、温度为18~20℃时，这种变化在完全黑暗条件下较完全光照条件下更为明显。表明羊脂酸除草机理可能与其抑制杂草的光合作用有关。

中文关键词:植物源除草剂羊脂酸杂草防控作用机理

Herbicidal activity and response mechanism of botanical caprylic acid

Author Name	Affiliation	E-mail
Li Zuren	Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Agricultural Biotechnology Reseach Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China Collaborative Innovation Center for Field Weeds Control, Loudi 417000, Hunan Provicne, China
Huang Qinqin	Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Agricultural Biotechnology Reseach Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China
Peng Qiong	Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Agricultural Biotechnology Reseach Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China Collaborative Innovation Center for Field Weeds Control, Loudi 417000, Hunan Provicne, China
Zhou Yong	Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Agricultural Biotechnology Reseach Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China
Zhou Xiaomao	Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Agricultural Biotechnology Reseach Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China Collaborative Innovation Center for Field Weeds Control, Loudi 417000, Hunan Provicne, China
Bai Lianyang	Hunan Provincial Key Laboratory for Biology and Control of Weeds, Hunan Agricultural Biotechnology Reseach Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, Hunan Province, China Collaborative Innovation Center for Field Weeds Control, Loudi 417000, Hunan Provicne, China	lybai196712@163.com

Abstract:In order to develop novel botanical herbicides, the natural products from plant with herbicidal activities were screened. Coconut were physically crushed to produce coconut oil, after saponification and acidification, coconut oil was distillated to several parts. The herbicidal part was identified by GSMS and evaluated by laboratory and field efficacy trials. The antioxidant enzyme (L-phenylalanin ammo-nialyase, polyphenol oxidase, peroxidase) activities of Conyza canadensis was measured after caprylic acid treatment at different concentrations and under varying light intensities. The results showed that the herbicidal part from coconut oil was identified to caprylic acid on GS-MS total ion chromatogram (6.20 min). The caprylic acid has good herbicidal activity (EC₅₀=14.07 mg/L). The control effects on the plant number and fresh weight reached 90.25% and 90.37% in a 20 m² field after 15 d caprylic acid EW application. Caprylic acid at low concentrations has no significant influenced on antioxidant enzyme activities in C. canadensis leaves. However, higher concentration of caprylic showed significant increasing on L-phenylalanin ammo-nialyase activity and significant decreasing on peroxidase and polyphenol oxidase activities. The effects on the three antioxidant activities in the light (24 h illuminations,100-120 μmol·m^-2·s^-1, 18-20℃) was more signification than in the dark. Therefore, the weed control mechanism of caprylic acid might be due to photosynthesis inhibition in weeds.

keywords:botanical herbicide caprylic acid weed control control mechanism

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