| 亚磷酸钾对马铃薯晚疫病病原菌的作用机理 |
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| 引用本文：林敏,王荣波,陈庆河,刘裴清,李本金,翁启勇.亚磷酸钾对马铃薯晚疫病病原菌的作用机理.植物保护学报,2018,45(6):1389-1395 |
| DOI：10.13802/j.cnki.zwbhxb.2018.2017094 |
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| 作者 | 单位 | E-mail | | 林敏 | 福建省农业科学院植物保护研究所, 福建省作物有害生物监测与治理重点实验室, 福州 350013 | | | 王荣波 | 福建省农业科学院植物保护研究所, 福建省作物有害生物监测与治理重点实验室, 福州 350013 | | | 陈庆河 | 福建省农业科学院植物保护研究所, 福建省作物有害生物监测与治理重点实验室, 福州 350013
福建农林大学, 闽台作物有害生物生态防控国家重点实验室, 福州 350002 | | | 刘裴清 | 福建省农业科学院植物保护研究所, 福建省作物有害生物监测与治理重点实验室, 福州 350013 | | | 李本金 | 福建省农业科学院植物保护研究所, 福建省作物有害生物监测与治理重点实验室, 福州 350013 | lbenjin@126.com | | 翁启勇 | 福建省农业科学院植物保护研究所, 福建省作物有害生物监测与治理重点实验室, 福州 350013
福建农林大学, 闽台作物有害生物生态防控国家重点实验室, 福州 350002 | wengqy@faas.cn |
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| 中文摘要:为明确亚磷酸钾对马铃薯晚疫病病原菌的作用机理，通过菌丝生长速率法和显微观察技术研究亚磷酸钾对马铃薯晚疫病病原菌菌丝生长和游动孢子释放的影响，并分别采用半定量反转录-聚合酶链锁反应（reverse transcription-polymerase chain reaction，RT-PCR）及生物化学技术分析亚磷酸钾处理后马铃薯叶片防御基因表达、氧化酶活性以及可溶性蛋白含量的变化情况。结果表明，亚磷酸钾对马铃薯晚疫病病原菌菌丝生长和游动孢子释放均有明显的抑制作用，其抑制中浓度EC50分别为24.03 μg/mL和5.99 μg/mL。接种晚疫病菌并经亚磷酸钾溶液处理的马铃薯叶片防御基因PR1、PR2表达量显著上调，接种8 d时表达量为银法利处理和清水对照的1.97~5.12倍；同时，过氧化物酶（peroxidase，POD）、超氧化物歧化酶（superoxide dismutase，SOD）、过氧化氢酶（catalase，CAT）酶活性增强，其峰值分别达534.29、281.13和68.84 U/mg，为银法利处理和清水对照的1.18~3.52倍；可溶性蛋白含量也随之增加，峰值（0.85 mg/mL）分别为银法利处理和清水对照的1.85倍和3.15倍。表明亚磷酸钾可通过直接抑菌作用和诱导植物产生抗病反应从而达到防治病害的目的。 |
| 中文关键词:亚磷酸钾 晚疫病病原菌 防御基因 氧化酶活性 可溶性蛋白含量 |
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| Action mechanism of potassium phosphite against potato Phytophthora infestans |
| Author Name | Affiliation | E-mail | | Lin Min | Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China | | | Wang Rongbo | Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China | | | Chen Qinghe | Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China | | | Liu Peiqing | Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China | | | Li Benjin | Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China | lbenjin@126.com | | Weng Qiyong | Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests, Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013, Fujian Province, China
State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China | wengqy@faas.cn |
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| Abstract:In order to explore the action mechanism of potassium phosphite against potato Phytophthora infestans, the method of mycelium growth rate and microscopic observation were used to detected the effect for mycelium growth and zoospore release of P. infestans treated with potassium phosphite, and the expression of defense genes, oxidase activity and soluble protein content in the treated leaves of potato were analyzed by RT-PCR and biochemical method, respectively, after being inoculated with P. infestans and application of the potassium phosphite. The results showed that potassium phosphite could inhibit the mycelium growth and zoospore release of P. infestans, and the EC50 values were 24.03, 5.99 μg/mL, respectively. The expression of resistance related genes, including PR1 and PR2, were significantly increased by 1.97-5.12 times than CK (infinito and water treatment), after application of potassium phosphite. The activity of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) were increased, the specific value of that were 534.29, 281.13, 68.84 U/mg, with 1.18-3.52 times than CK, and the soluble protein concentration were also increased, the specific value of that were 0.85 mg/mL, with 1.85 and 3.15 times than CK (infinito and water treatment), respectively. In conclusion, the potassium phosphite may against the pathogen of oomycetes by direct inhibition and induced resistance of host. |
| keywords:potassium phosphite Phytophthora infestans defense genes oxidase activity soluble protein content |
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