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河北省和山东省黄瓜霜霉病菌对氟吡菌胺的抗性及常规药剂对黄瓜霜霉病的田间防效

引用本文：王文桥,韩秀英,吴杰,赵建江,孟润杰,路粉.河北省和山东省黄瓜霜霉病菌对氟吡菌胺的抗性及常规药剂对黄瓜霜霉病的田间防效.植物保护学报,2019,46(2):385-392

DOI：10.13802/j.cnki.zwbhxb.2019.2017220

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作者	单位	E-mail
王文桥	河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000	wenqiaow@163.com
韩秀英	河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000
吴杰	河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000
赵建江	河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000
孟润杰	保定职业技术学院, 河北保定 071051
路粉	河北省农林科学院植物保护研究所, 河北省农业有害生物综合防治工程技术研究中心, 农业部华北北部作物有害生物综合治理重点实验室, 保定 071000

中文摘要:为明确黄瓜霜霉病菌Pseudoperonospora cubensis对氟吡菌胺的抗性时空动态，2011—2016年从河北省和山东省黄瓜主产区采集1 821株霜霉病菌，采用叶盘漂浮法检测供试菌株对氟吡菌胺的敏感性，并以茎叶喷雾法评估5种常规药剂对黄瓜霜霉病的田间防效。结果表明，河北省和山东省黄瓜主产区的黄瓜霜霉病菌对氟吡菌胺已普遍产生了低度抗性，平均抗性倍数为5.86；所有供试菌株的抗性频率为51.89%，其中低抗菌株及中抗菌株分别占36.18%和15.71%；平均抗性指数为0.34，每年检测的抗性频率、抗性倍数及抗性指数呈增长趋势，且随着监测区域的变化而变化。在河北省定兴县和山东省寿光市进行的田间防效试验结果显示，按推荐剂量喷施，687.5 g/L氟吡菌胺·霜霉威盐酸盐SC对黄瓜霜霉病防效显著高于常规对照药剂68%精甲霜灵·代森锰锌WG、58%甲霜灵·代森锰锌WP、80%代森锰锌WP及250 g/L嘧菌酯SC的防效，但防效明显下降，由2011年的92.58%~93.31%降至2016年的80.07%~80.82%。表明需要制定和实施抗药性治理对策，如限制山东和河北2省黄瓜的每个生长季节中687.5 g/L氟菌·霜霉威SC的使用不超过2次，而且要与不同作用机理的卵菌杀菌剂交替使用防治黄瓜霜霉病。

中文关键词:黄瓜霜霉病菌氟吡菌胺抗性时空动态田间防效

Resistance of Pseudoperonospora cubensis to fluopicolide and control efficacy of conventional fungicides against cucumber downy mildew in Hebei and Shandong provinces, China

Author Name	Affiliation	E-mail
Wang Wenqiao	Key Laboratory for Integrated Management of Crop Pests in Northern Regions of North China, Ministry of Agriculture and Rural Affairs, Integrated Pest Managment Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China	wenqiaow@163.com
Han Xiuying	Key Laboratory for Integrated Management of Crop Pests in Northern Regions of North China, Ministry of Agriculture and Rural Affairs, Integrated Pest Managment Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China
Wu Jie	Key Laboratory for Integrated Management of Crop Pests in Northern Regions of North China, Ministry of Agriculture and Rural Affairs, Integrated Pest Managment Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China
Zhao Jianjiang	Key Laboratory for Integrated Management of Crop Pests in Northern Regions of North China, Ministry of Agriculture and Rural Affairs, Integrated Pest Managment Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China
Meng Runjie	Baoding College of Technology, Baoding 071051, Hebei Province, China
Lu Fen	Key Laboratory for Integrated Management of Crop Pests in Northern Regions of North China, Ministry of Agriculture and Rural Affairs, Integrated Pest Managment Center of Hebei Province, Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences, Baoding 071000, Hebei Province, China

Abstract:To clarify the temporal and spatial dynamics of the resistance Pseudoperonospora cubensis to fluopicolide in Hebei and Shandong provinces of China, the sensitivities to fluopicolide in 1 821 P. cubensis strains collected from main cucumber production areas of Hebei and Shandong provinces from 2011 to 2016 were measured by leaf disc floating tests, and the control efficacies of five conventional fungicides against cucumber downy mildew were assayed by spraying stems and leaves in the fields. The results showed that P. cubensis widely developed low resistance to fluopicolide all over the main cucumber production areas of Hebei and Shandong, with an average resistance factor of 5.86. The strains with low and moderate resistance accounted for 36.18% and 15.71%, respectively; the resistance frequency to fluopicolide was 51.89%, and the average resistance index was 0.34 for all tested strains. The three indexes of P. cubensis tended to increase year by year and varied as the detected regions changed. The results of the trials of control efficacy of fungicides against the disease showed that spray of fluopicolide·propamocarb hydrochloride 687.5 g/L SC at the recommended rate gave significantly higher control against cucumber downy mildew in Hebei and Shandong than spray of mefenoxam·mancozeb 68 WG, metalaxyl·mancozeb 58 WP, mancozeb 80 WP or azoxystrobin 250 g/L SC at the recommended rates, but the control efficacy of fluopicolide·propamocarb hydrochloride 687.5 g/L SC significantly decreased from 92.58%-93.31% in 2011 to 80.07%-80.82% in 2016. The monitoring results of wide occurrence of low resistance to fluopicolide was consistent with the field trial result of obviously less effectiveness of fluopicolide·propamocarb hydrochloride 687.5 g/L SC, indicating that the strategies of resistance management to fluopicolide in P. cubensis need to be established and implemented, such as limiting the spray times of fluopicolide·propamocarb hydrochloride 687.5 g/L SC to twice within a growth season of cucumber and using fluopicolide·propamocarb hydrochloride 687.5 g/L SC in alternation with the other oomycete fungicides with different modes of action for control of cucumber downy mildew in Hebei and Shandong provinces.

keywords:Pseudoperonospora cubensis fluopicolide resistance temporal and spatial dynamics control efficacy

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