| 芥菜乙醇提取物对西瓜枯萎病菌的抑菌机制 |
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| 引用本文：刘琳,王强,袁仁文,张蕊,王静,范淑英.芥菜乙醇提取物对西瓜枯萎病菌的抑菌机制.植物保护学报,2019,46(4):816-823 |
| DOI：10.13802/j.cnki.zwbhxb.2019.2019002 |
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| 中文摘要:为探究芥菜乙醇提取物对植物病原菌的抑制机理，以西瓜枯萎病菌Fusarium oxysporum f.sp.niveum为指示菌，以最低抑菌浓度（minimum inhibitory concentration，MIC）0.5 g/mL为基准，设置0、0.25、0.5和1.0 g/mL共4种处理浓度，通过测定菌丝形态结构、菌液电导率、菌体丙二醛含量、细胞壁相关水解酶活性、呼吸代谢途径酶活性、可溶性蛋白含量及还原糖含量等指标的变化分析其抑菌机理。结果显示，1.0 g/mL芥菜乙醇提取物对西瓜枯萎病菌的处理效果最好，其中，处理后病菌菌液的电导率明显增大，最大可达1.45 ms/cm，是对照组的1.57倍；丙二醛含量达3.16 mmol/g，为对照组的2.24倍；几丁质酶和β-1，3-葡聚糖酶的活性最高，分别为10.57 U/g和14.16 U/g，较对照组分别显著升高了48.04%和17.80%，同时细胞壁降解，使细胞完整性受损；琥珀酸脱氢酶和苹果酸脱氢酶的活性最低，分别为7.33 U/mg和0.28 U/mg，较对照组分别显著下降了73.02%和47.17%；可溶性蛋白和还原糖的含量显著降低，分别为0.44 mg/g和5.34 mg/g，较对照组分别下降了60.71%和56.30%，呼吸能量代谢受阻，抑制菌体生长。表明芥菜乙醇提取物可有效防治西瓜枯萎病。 |
| 中文关键词:芥菜 西瓜枯萎病 抑菌机理 |
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| Suppression mechanism of the ethanol extracts from Brassica juncea against the pathogen of watermelon Fusarium wilt |
| Author Name | Affiliation | E-mail | | Liu Lin | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Wang Qiang | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Ruan Renwen | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Zhang Rui | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Wang Jing | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | | | Fan Shuying | College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China | fansy12@126.com |
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| Abstract:In order to explore the inhibitory mechanism of the extract of Brassica juncea against plant pathogenic bacteria, Fusarium oxysporum f. sp. niveum was used as an indicator bacterium, and the minimum inhibitory concentration (MIC) 0.5 g/mL was used as the benchmark to set 0, 0.25, 0.5 and 1.0 g/mL treatments, by measuring the morphology of mycelium, changes in bacterial liquid conductivity, bacterial malondialdehyde content, cell wall-related hydrolase activity, respiratory metabolic pathway enzyme activity, soluble protein content and reducing sugar content to analyze its antibacterial mechanism. The results showed that the conductivity of the bacterial liquid of F. oxysporum f. sp. niveum after the treatment of the B. juncea ethanol extract increased significantly, and the conductivity of the bacterial liquid at the concentration of 1.0 g/mL was up to 1.45 ms/cm, which was 1.57 times the control, and malondialdehyde content reached 3.16 mmol/g, 2.24 times the control. The chitinase and β-1,3-glucanase activities were 10.57 U/g and 14.16 U/g, respectively, which were significantly higher than the control by 48.04% and 17.80%, accompanied by cell wall degradation and impaired cell integrity. The succinate dehydrogenase and malate dehydrogenase activities were 7.33 U/mg and 0.28 U/mg, respectively, which were significantly lower than the control by 73.02% and 47.17%. The contents of cell contents such as soluble protein, reducing sugar content were significantly reduced to 0.44 mg/g and 5.34 mg/g, decreased by 60.71% and 56.30%, accompanied by blocked respiratory energy metabolism and inhibited cell growth. It indicated that B. juncea ethanol extracts could effectively control watermelon Fusarium wilt. |
| keywords:Brassica juncea watermelon Fusarium wilt antifungal mechanism |
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