| 稻稗HJHL-715种群对五氟磺草胺的抗药性水平及抗性机理分析 |
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| 引用本文：刘健,房加鹏,董立尧.稻稗HJHL-715种群对五氟磺草胺的抗药性水平及抗性机理分析.植物保护学报,2020,47(1):197-204 |
| DOI：10.13802/j.cnki.zwbhxb.2020.2019034 |
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| 中文摘要:为明确东北稻区稻稗Echinochloa oryzoides HJHL-715种群对五氟磺草胺的抗性水平及抗性机制，采用整株生物测定法测定稻稗种群对五氟磺草胺的敏感性，明确抗性种群的交互抗性和多抗性情况，研究3种细胞色素P450抑制剂对其敏感性的影响；并应用分子生物学方法进行稻稗的乙酰乳酸合酶（acetolactate synthase，ALS）离体活性测定、ALS基因序列分析及其表达量测定。结果表明：在东北稻区，五氟磺草胺对稻稗HJHL-715种群鲜重的抑制中剂量GR50为62.53 g/hm2；稻稗HJHL-715的ALS基因序列中未发现氨基酸突变，其ALS离体活性与敏感种群的ALS离体活性无显著性差异，ALS基因表达量显著低于敏感种群。1-氨基苯并三唑（1-aminobenzotriazole，ABT）、胡椒基丁醚（piperomyl butoxide，PBO）、马拉硫磷3种P450抑制剂显著提高了稻稗HJHL-715种群对五氟磺草胺的敏感性，使其对五氟磺草胺的GR50由原来的62.53 g/hm2分别下降到5.78、5.02、3.53 g/hm2。表明东北稻区已经出现了对五氟磺草胺具有高水平抗性的稻稗种群，稻稗HJHL-715种群对五氟磺草胺的抗性很可能是由细胞色素P450介导的代谢增强所致。 |
| 中文关键词:稻稗 五氟磺草胺 乙酰乳酸合酶 P450代谢 |
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| Analysis of the resistance level and its mechanism of barnyard grass Echinochloa oryzoides population HJHL-715 to penoxsulam |
| Author Name | Affiliation | E-mail | | LIU Jian | Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, College of Plant Protection, Nanjing 210095, Jiangsu Province, China | | | FANG Jiapeng | Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, College of Plant Protection, Nanjing 210095, Jiangsu Province, China | | | DONG Liyao | Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, College of Plant Protection, Nanjing Agricultural University, College of Plant Protection, Nanjing 210095, Jiangsu Province, China | dly@njau.edu.cn |
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| Abstract:To clarify the resistance of barnyard grass Echinochloa oryzoides population HJHL-715 to penoxsulam and its resistance mechanism in northeast China, whole-plant pot bioassays were conducted to determine the sensitivity of E. oryzoides to penoxsulam, and to explore its cross-resistance and multiresistance patterns and the effects of three cytochrome P450 inhibitors on its sensitivity to penoxsulam. The ALS gene sequence analysis, ALS gene expression and in vitro acetolactate synthase activity of E. oryzoides were investigated by molecular biological methods. The fresh quality inhibition medium dose (GR50) of the population HJHL-715 to penoxsulam in northeast China was 62.53 g/hm2, showing the high resistance level. No amino acid substitutions were found through the alignment of ALS gene sequences. There was no significant difference in the in vitro ALS activity between sensitive and resistant populations. Resistant populations had lower gene expression levels of ALS than sensitive populations. Three P450 inhibitors including 1-aminobenzotriazole (ABT), piperomyl butoxide (PBO) and malathion significantly increased the sensitivity of population HJHL-715 to penoxsulam. The GR50 of the population HJHL-715 decreased significantly from 62.53 g/hm2 to 5.78, 5.02, and 3.53 g/hm2, respectively. These results suggested that the resistance of E. oryzoides population HJHL-715 to penoxsulam in northeast China was mostly due to enhanced herbicide metabolism, possibly via cytochrome P450. |
| keywords:Echinochloa oryzoides penoxsulam acetolactate synthase P450 metabolism |
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