| 菊酯类药剂对草地贪夜蛾的毒力及对钠离子通道的诱导特性 |
| 点此下载全文 |
| 引用本文：李芬,王力奎,彭正强,MALHAT Farag,吕宝乾,吴少英.菊酯类药剂对草地贪夜蛾的毒力及对钠离子通道的诱导特性.植物保护学报,2020,47(4):884-890 |
| DOI：10.13802/j.cnki.zwbhxb.2020.2020826 |
| 摘要点击次数: |
| 全文下载次数: |
|
|
| 中文摘要:为明确菊酯类药剂对草地贪夜蛾Spodoptera frugiperda的防治效果，采用点滴法在室内测定高效氯氟氰菊酯、高效氯氰菊酯、联苯菊酯、溴氰菊酯和茚虫威原药对草地贪夜蛾3龄幼虫的毒力，利用实时荧光定量PCR技术测定3龄幼虫在这5种药剂诱导不同时间下其体内钠离子通道基因片段的表达量变化。结果显示，高效氯氟氰菊酯、溴氰菊酯、联苯菊酯、茚虫威和高效氯氰菊酯这5种药剂处理草地贪夜蛾3龄幼虫24 h后的LD50值分别为74.911、83.280、152.662、179.372、567.250 mg/L；处理48 h后的LD50值分别为18.946、67.874、120.888、132.790、461.635 mg/L；同时发现草地贪夜蛾3龄幼虫钠离子通道基因的表达均呈现不被诱导或诱导下调现象，表明草地贪夜蛾幼虫可通过降低靶标基因的表达量进而减少药剂对其的毒性。表明供试4种拟除虫菊酯类药剂和1种氯虫苯甲酰胺类药剂可作为草地贪夜蛾田间应急防控药剂施用。 |
| 中文关键词:海南省 草地贪夜蛾 杀虫剂 拟除虫菊酯 茚虫威 |
| |
| Toxicity of pyrethroid pesticides to fall armyworm Spodoptera frugiperda and its inducing properties of sodium ion channels |
| Author Name | Affiliation | E-mail | | LI Fen | College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China | | | WANG Likui | College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China | | | PENG Zhengqiang | Environment and Plant Protection Institute, China Academy of Tropical Agricultural Sciences, Haikou 570228, Hainan Province, China | | | MALHAT Farag | Central Agricultural Pesticide Laboratory, Pesticide Residues and Environmental Pollution Department, Agricultural Research Center, Giza 12618, Dokki, Egypt | | | Lü Baoqian | Environment and Plant Protection Institute, China Academy of Tropical Agricultural Sciences, Haikou 570228, Hainan Province, China | lvbaoqian@hotmail.com | | WU Shaoying | College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China | wsywsy6000@hainanu.edu.cn |
|
|
| Abstract:In order to clear the control effect of pyrethroid pesticides to fall armyworm Spodoptera frugiperda, the toxicity of lambda-cyhalothrin, beta cypermethrin, bifenthrin, deltamethrin and indoxacarb against the third instar larvae of S. frugiperda by drop method and the expression level of sodium channel gene in the third instar larvae under the induction of these five insecticides was detected by quantitative real-time PCR. The results showed that the LD50 values of lambda-cyhalothrin, deltamethrin, bifenthrin, indoxacarb and beta cypermethrin after 24 h treatment of the third instar larvae of S. frugiperda were 74.911, 83.280, 152.662, 179.372 and 567.250 mg/L, respectively. After 48 h of treatment, the LD50 values were in the order of 18.946, 67.874, 120.888, 132.790 and 461.635 mg/L, respectively. Meanwhile, the expression level of sodium channel gene was showed no change or down-regulated. It indicated that the fall armyworm larvae could reduce the toxicity of the insecticides by reducing the expression of the target gene. This study was preliminary cleared that the indoor insecticidal activity of four pyrethroid insecticides and one chlorobiobenzoamide insecticides and revealed these insecticides can be used as field emergency prevention and control of S. frugiperda at present. |
| keywords:Hainan Province Spodoptera frugiperda insecticide pyrethroid indoxacarb |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 8740129 版权所有：植物保护学报 京ICP备05006550号-2 主管单位：中国科协 主办单位：中国植物保护学会、中国农业大学 地址：北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话：010-62732528 电子邮件：zbxb@cau.edu.cn 技术支持： 北京勤云科技发展有限公司 |
