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多杀霉素亚致死浓度对棉铃虫幼虫生理及代谢的影响

引用本文：宋永辉,徐梦超,薛玉莹,魏纪珍,杜孟芳,尹新明,刘晓光,安世恒.多杀霉素亚致死浓度对棉铃虫幼虫生理及代谢的影响.植物保护学报,2021,48(5):1156-1163

DOI：10.13802/j.cnki.zwbhxb.2021.2021868

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作者	单位	E-mail
宋永辉	河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002
徐梦超	河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002
薛玉莹	河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002
魏纪珍	河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002
杜孟芳	河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002
尹新明	河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002
刘晓光	河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002	xgliu2000@aliyun.com
安世恒	河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002

中文摘要:为明确田间使用多杀霉素亚致死浓度对棉铃虫Helicoverpa armigera幼虫的影响，用含多杀霉素亚致死浓度LC₂₅的人工饲料持续饲喂棉铃虫3龄幼虫，并对饲喂后其体重、取食量、累计蛹化率、蛹发育历期和蛹重等生长发育及脂肪体内甘油三脂（triglyceride，TG）含量和相关基因SREBP、FAS和HSL表达情况进行测定。结果表明，多杀霉素对棉铃虫的亚致死浓度LC₂₅为0.21 mg/kg；多杀霉素亚致死浓度处理4~6 d后，棉铃虫3龄幼虫体重分别为0.065、0.263和0.329 g，较对照显著降低；处理6 d后，其取食量为0.082 g，较对照显著降低；处理4~7 d后，其累计化蛹率分别为60.90%、63.20%、65.50%和65.50%，较对照显著降低。多杀霉素亚致死浓度处理后，棉铃虫蛹发育历期由对照9.89 d显著延长至10.74 d，单头蛹重为0.274 g，显著低于对照的0.324 g；其脂肪体TG含量较对照显著降低。多杀霉素亚致死浓度处理24~72 h后，参与脂肪酸合成信号通路中重要基因SREBP和FAS的相对表达量较对照均显著下调，而参与脂肪代谢的重要基因HSL则较对照显著上调。

中文关键词:棉铃虫亚致死浓度多杀霉素生长发育生理代谢脂肪体相关基因

Effects of sublethal concentration of spinosad on the metabolism and physiology of cotton bollworm Helicoverpa armigera

Author Name	Affiliation	E-mail
Song Yonghui	State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China
Xu Mengchao	State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China
Xue Yuying	State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China
Wei Jizhen	State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China
Du Mengfang	State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China
Yin Xinming	State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China
Liu Xiaoguang	State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China	xgliu2000@aliyun.com
An Shiheng	State Key Laboratory of Wheat and Maize Crop Science, College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China

Abstract:In order to investigate the effects of sublethal doses of pesticides on cotton bollworm Helicoverpa armigera in crop fields, the artificial diets mixed with the LC₂₅ of biopesticide spinosad were fed from the 3rd instar larva to pupal stage of H. armigera. The physiological indexes such as body weight, food consumption weight, the accumulative total pupation rate, developmental duration and pupal weight were observed, and triglyceride (TG) titer, relative expressions of different genes such as FAS, SREBP, HSL associated with the biosynthesis of fatty acids and lipid homeostasis pathways in the fat body were measured. The results showed that sublethal dose of spinosad LC₂₅ was 0.21 mg/kg, the body weights of the 3rd instar larvae of H. armigera fed with the artificial diets mixed with the LC₂₅ of spinosad from the fourth to sixth days were 0.065, 0.263 and 0.329 g, respectively, and the food consumption at the sixth day was 0.082 g, which all decreased compared with the control group. The accumulated pupation rates of the 3rd instar larvae of H. armigera fed with the artificial diets mixed with spinosad were 60.90%, 63.20%, 65.50% and 65.50%, respectively, from the 4th to 7th days, which were decreased compared with the control population, and the pupation stage was prolonged from 9.89 d (control) to 10.74 d (fed with the artificial diet mixed with spinosad). The content of TG in fatty tissues was also lower than that of the control from 24 h to 72 h after fed with sublethal concentration of spinosad, and the gene expression levels of SREBP and FAS were all down-regulated, while HSL was up-regulated.

keywords:Helicoverpa armigera sublethal concentration spinosad growth and development physiology metabolism fat body relative gene

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