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黄瓜绿斑驳花叶病毒的遗传多样性与检测技术研究进展

引用本文：罗金燕,吴志峰,谢婷,陈磊,杜伟,李斌.黄瓜绿斑驳花叶病毒的遗传多样性与检测技术研究进展.植物保护学报,2022,49(2):460-470

DOI：10.13802/j.cnki.zwbhxb.2022.2021048

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作者	单位	E-mail
罗金燕	上海市农业技术推广服务中心, 上海 201103
吴志峰	浙江大学生物技术研究所, 杭州 310058
谢婷	浙江大学生物技术研究所, 杭州 310058
陈磊	上海市农业技术推广服务中心, 上海 201103
杜伟	宁夏回族自治区农业技术推广总站, 银川 750001
李斌	浙江大学生物技术研究所, 杭州 310058	libin0571@zju.edu.cn

中文摘要:黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)是我国重要的植物检疫性有害生物,预防其扩散和传播的关键在于准确、灵敏和快速的检测技术。该文总结分析NCBI数据库中已测定CGMMV分离物的基因组多样性;比较目前CGMMV主要检测技术的优缺点;归纳总结当前广泛用于检测CGMMV的逆转录-聚合酶链反应(reverse transcription PCR,RT-PCR)引物,并针对RT-PCR技术的最新研究进展和存在的一些不足进行讨论;最后对CGMMV检测技术的未来发展方向进行展望。

中文关键词:黄瓜绿斑驳花叶病毒检疫基因组遗传多样性检测技术 RT-PCR

Research progresses on genetic diversity and detection technology of cucumber green mottle mosaic virus

Author Name	Affiliation	E-mail
LUO Jin-yan	ShanghaiAgriculture Technology Extension & Service Center, Shanghai 201103, China
WU Zhi-feng	Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
XIE Ting	Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China
CHEN Lei	ShanghaiAgriculture Technology Extension & Service Center, Shanghai 201103, China
DU Wei	Agricultural Technology Extension Station of Ningxia Hui Autonomous Region, Yinchuan 750001, Ningxia Hui Autonomous Region, China
LI Bin	Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China	libin0571@zju.edu.cn

Abstract:Cucumber green mottle mosaic virus(CGMMV) is an important plant quarantine pest in China. The key to prevent its spread lies in accurate, sensitive and rapid detection technology. This paper firstly analyzed and summarized the diversity of the sequenced CGMMV genomes available in the NCBI database; compared the advantages and disadvantages of the current main CGMMV detection technologies; summed up the CGMMV detection primers that widely used in reverse transcription PCR(RTPCR), and then discussed the latest research progresses and some shortcomings of RT-PCR technology.Finally, this paper looked forward to the future direction of CGMMV detection technology.

keywords:cucumber green mottle mosaic virus quarantine genome genetic diversity detection technology RT-PCR

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