黄瓜绿斑驳花叶病毒的遗传多样性与检测技术研究进展 |
点此下载全文 |
引用本文:罗金燕,吴志峰,谢婷,陈磊,杜伟,李斌.黄瓜绿斑驳花叶病毒的遗传多样性与检测技术研究进展.植物保护学报,2022,49(2):460-470 |
DOI:10.13802/j.cnki.zwbhxb.2022.2021048 |
摘要点击次数: |
全文下载次数: |
|
|
中文摘要:黄瓜绿斑驳花叶病毒(cucumber green mottle mosaic virus,CGMMV)是我国重要的植物检疫性有害生物,预防其扩散和传播的关键在于准确、灵敏和快速的检测技术。该文总结分析NCBI数据库中已测定CGMMV分离物的基因组多样性;比较目前CGMMV主要检测技术的优缺点;归纳总结当前广泛用于检测CGMMV的逆转录-聚合酶链反应(reverse transcription PCR,RT-PCR)引物,并针对RT-PCR技术的最新研究进展和存在的一些不足进行讨论;最后对CGMMV检测技术的未来发展方向进行展望。 |
中文关键词:黄瓜绿斑驳花叶病毒 检疫 基因组 遗传多样性 检测技术 RT-PCR |
|
Research progresses on genetic diversity and detection technology of cucumber green mottle mosaic virus |
Author Name | Affiliation | E-mail | LUO Jin-yan | ShanghaiAgriculture Technology Extension & Service Center, Shanghai 201103, China | | WU Zhi-feng | Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China | | XIE Ting | Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China | | CHEN Lei | ShanghaiAgriculture Technology Extension & Service Center, Shanghai 201103, China | | DU Wei | Agricultural Technology Extension Station of Ningxia Hui Autonomous Region, Yinchuan 750001, Ningxia Hui Autonomous Region, China | | LI Bin | Institute of Biotechnology, Zhejiang University, Hangzhou 310058, Zhejiang Province, China | libin0571@zju.edu.cn |
|
|
Abstract:Cucumber green mottle mosaic virus(CGMMV) is an important plant quarantine pest in China. The key to prevent its spread lies in accurate, sensitive and rapid detection technology. This paper firstly analyzed and summarized the diversity of the sequenced CGMMV genomes available in the NCBI database; compared the advantages and disadvantages of the current main CGMMV detection technologies; summed up the CGMMV detection primers that widely used in reverse transcription PCR(RTPCR), and then discussed the latest research progresses and some shortcomings of RT-PCR technology.Finally, this paper looked forward to the future direction of CGMMV detection technology. |
keywords:cucumber green mottle mosaic virus quarantine genome genetic diversity detection technology RT-PCR |
查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 8618612 版权所有:植物保护学报 京ICP备05006550号-2 主管单位:中国科协 主办单位:中国植物保护学会、中国农业大学 地址:北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话:010-62732528 电子邮件:zbxb@cau.edu.cn 技术支持: 北京勤云科技发展有限公司
|