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野生甘蓝表皮毛对菜青虫的抗性机理
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引用本文:李魁,马骁,王志敏,钱伟,梅家琴,汤青林,魏大勇.野生甘蓝表皮毛对菜青虫的抗性机理.植物保护学报,2022,49(2):662-670
DOI:10.13802/j.cnki.zwbhxb.2021.2020163
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作者单位E-mail
李魁 西南大学园艺园林学院, 南方山地园艺学教育部重点实验室, 重庆 400715  
马骁 西南大学园艺园林学院, 南方山地园艺学教育部重点实验室, 重庆 400715  
王志敏 西南大学园艺园林学院, 南方山地园艺学教育部重点实验室, 重庆 400715  
钱伟 西南大学农学与生物科技学院, 重庆 400715  
梅家琴 西南大学农学与生物科技学院, 重庆 400715  
汤青林 西南大学园艺园林学院, 南方山地园艺学教育部重点实验室, 重庆 400715 swutql@163.com 
魏大勇 西南大学园艺园林学院, 南方山地园艺学教育部重点实验室, 重庆 400715 swuwdy@swu.edu.cn 
中文摘要:为明确一种全身覆盖表皮毛的野生甘蓝Brassica incana (编号C01)是否具有抗虫性,通过测定菜青虫Pieris rapae对野生甘蓝C01和无毛甘蓝B.alboglabra(编号C41)的拒食、取食和产卵行为进行抗虫性分析,同时通过测定两者的内源激素含量、表皮毛发育相关基因表达量和防御酶活性探讨野生甘蓝C01对菜青虫的抗性机理。结果显示,生长至8~10叶期,无毛甘蓝C41叶片被菜青虫啃食严重,但野生甘蓝C01叶片未被啃食;菜青虫对无毛甘蓝C41和剪除表皮毛的野生甘蓝C01叶片取食面积差异不显著,但均显著大于对野生甘蓝C01叶片的取食面积;着卵的无毛甘蓝C41植株显著多于野生甘蓝C01。野生甘蓝C01叶片中茉莉酸和茉莉酸甲酯含量都显著高于无毛甘蓝C41叶片,而两者中水杨酸和水杨酸甲酯的含量差异不显著。BolJAZ1基因在无毛甘蓝C41叶片中高表达,而BolGL3BolGL2基因在野生甘蓝C01叶片中高表达;且野生甘蓝C01叶片中多酚氧化酶、过氧化物酶和苯丙氨酸解氨酶3种防御酶的活性均显著高于无毛甘蓝C41。表明野生甘蓝C01叶片的表皮毛会影响菜粉蝶产卵,对菜青虫表现出显著抗性,同时野生甘蓝C01叶片中内源激素茉莉酸和茉莉酸甲酯的增加以及BolGL3/BolGL2基因的高表达显示茉莉酸途径可能参与了甘蓝表皮毛的分化。
中文关键词:甘蓝  表皮毛  菜青虫  激素  抗性
 
The mechanism of trichome resistance in wild Brassica oleracea to cabbage white butterfly Pieris rapae
Author NameAffiliationE-mail
LI Kui Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China  
MA Xiao Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China  
WANG Zhi-min Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China  
QIAN Wei College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China  
MEI Jia-qin College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China  
TANG Qing-lin Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China swutql@163.com 
WEI Da-yong Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China swuwdy@swu.edu.cn 
Abstract:To determine whether a wild type of Brassica incana (coded as C01) covered with trichomes was insect-resistant, the insect resistance analysis of C01 and hairless B. alboglabra (coded as C41) was performed by measuring the antifeeding effect, feeding effect and the spawning behavior of cabbage white butterfly Pieris rapae. Meanwhile, to explore the mechanism of C01 resistance to P. rapae, the endogenous hormone content, gene expression patterns related to trichome development and the activity of defense enzymes in C01 and C41 were further determined. The results showed that the leaves of C41 were more seriously eaten than C01 by P. rapae during the 8- to 10-leaf stage. There was no significant difference in the areas eaten by P. rapae between the leaves of C41 and C01 with trichomes cut off, but the leaf area of C41 eaten by P. rapae was significantly larger than that of wild C01. The number of eggs in C41 was significantly greater than that in C01. In addition, the average contents of jasmonic ac id (JA) and methyl jasmonate (MeJA) in C01 leaves were significantly higher than those in C41 leaves, while the average contents of salicylic acid and methyl salicylate had no significant difference between the two types of leaves. Gene expression analysis indicated that BolJAZ1 was highly expressed in C41 leaves, while BolGL3 and BolGL2 were highly expressed in C01 leaves. The activities of polyphenol oxidase, peroxidase and phenylalanine ammonia-lyase in C01 leaves were significantly higher than those in C41 leaves. In brief, the trichome of C01 affected the spawning of the butterfly, and thus showed significant resistance to P. rapae. Meanwhile, the increase of endogenous JA and MeJA in C01 leaves and the high expression of BolGL3/BolGL2 genes suggested that the JA pathway might be involved in the differentiation of trichome in B. oleracea.
keywords:Brassica oleracea  trichome  Pieris rapae  hormone  resistance
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