| 褐飞虱内共生菌研究进展 |
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| 引用本文：李慕雨,程依情,王正亮,俞晓平.褐飞虱内共生菌研究进展.植物保护学报,2023,50(2):274-286 |
| DOI：10.13802/j.cnki.zwbhxb.2023.2021139 |
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| 中文摘要:褐飞虱Nilaparvata lugens是我国及东南亚地区水稻上的主要害虫,其体内存在的大量共生菌是褐飞虱生物防治中尚未开发的一类重要微生物资源。褐飞虱内共生菌种类丰富多样,目前研究主要集中于其初级共生真菌——类酵母共生菌(yeast-like symbiotes,YLS)和2类次级共生细菌——沃尔巴克氏体Wolbachia和杀雄菌Arsenophonus。早期关于褐飞虱内共生菌的分离及种类鉴定主要采用体外离体培养、克隆文库构建以及PCR-变性梯度凝胶电泳等传统技术。近年来,宏基因组和高通量测序技术的应用极大拓展了褐飞虱内共生菌种类多样性研究的深度。内共生菌不仅参与褐飞虱的营养代谢,为宿主提供氨基酸、维生素和甾醇等必需营养物质以满足宿主正常的生长发育和繁殖,同时还可以介导宿主的致害性变异、抗药性产生和病原物防御等生理过程。鉴于内共生菌在褐飞虱生长发育及环境适应过程中的重要作用,利用内共生菌对褐飞虱进行生物防控已成为目前研究的一大热点,并展现出良好的应用前景。该文对褐飞虱内共生菌的主要种类及其生物学特性进行介绍,对其分离鉴定技术和多样性分析技术进行总结,并对褐飞虱内共生菌的生物学功能进行系统综述,最后对内共生菌在褐飞虱生物防治过程中的应用潜能及存在问题进行探讨,并对今后褐飞虱内共生菌相关研究进行展望。 |
| 中文关键词:褐飞虱 内共生菌 多样性 生物学功能 生物防治 |
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| Research progresses in endosymbionts of the brown planthopper Nilaparvata lugens (Hemiptera: Delphacidae) |
| Author Name | Affiliation | E-mail | | Li Muyu | Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang Province, China | | | Cheng Yiqing | Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang Province, China | | | Wang Zhengliang | Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang Province, China | zhengliang.w0234@163.com | | Yu Xiaoping | Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou 310018, Zhejiang Province, China | yuxiaoping19630306@163.com |
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| Abstract:The brown planthopper (BPH), Nilaparvata lugens, a major pest of rice in China and Southeast Asia, harbors a large number of endosymbionts, which are considered as important microbial resources that can be exploited and utilized in BPH biocontrol. The endosymbionts in BPH are rich in species. Among them, the primary fungal endosymbionts namely yeast-like symbiotes and two groups of bacterial endosymbionts Wolbachia and Arsenophonus were focused at present. Traditional methods for isolation and identification of BPH endosymbionts mainly include in vitro culture technique, cloning library construction and PCR-denaturing gradient gel electrophoresis. Recently, the depth of research on BPH endosymbiont diversity was greatly expanded by the application of metagenomics and highthroughput sequencing technologies. Endosymbionts are not only involved in host nutritional metabolism and hence provide essential nutrients, such as amino acids, vitamins and sterols for host growth, development and reproduction, but also mediate many physiological processes including virulence variation, insecticide resistance and pathogen defense. In view of the key roles that endosymbionts play in host growth, development and environmental adaptation, the endosymbiont-based biocontrol strategies for BPH have drawn much attention in recent years and shown a promising prospect for application. This review first gave a brief description of the main species and their biological characteristics of BPH endosymbionts, and then summarized the techniques used for the isolation, identification and diversity analysis of endosymbionts. Next, the biological functions of endosymbionts performed in BPH were systematically reviewed. Finally, the application potentials of endosymbionts in the biocontrol of BPH as well as the exsiting problems and future perspects were discussed. |
| keywords:Nilaparvata lugens endosymbiont diversity biological function biocontrol |
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