• 首页 | 期刊简介  | 
    编委会
    编委会
    青年编委会
     | 道德声明 | 投稿指南 | 联系我们 | 期刊订阅 | English
2019—2020年河南省小麦茎基腐病病原菌鉴定及致病力测定
点此下载全文
引用本文:高飞,谢源,潘鑫,李洪连,张晓婷,吴祖建,关雄.2019—2020年河南省小麦茎基腐病病原菌鉴定及致病力测定.植物保护学报,2023,50(2):298-305
DOI:10.13802/j.cnki.zwbhxb.2023.2022050
摘要点击次数:
全文下载次数:
作者单位E-mail
高飞 福建农林大学植物保护学院, 福州 350002
河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002 
 
谢源 福建农林大学植物保护学院, 福州 350002
河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002 
 
潘鑫 河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002  
李洪连 河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002  
张晓婷 河南农业大学植物保护学院, 省部共建小麦玉米作物学国家重点实验室, 郑州 450002  
吴祖建 福建农林大学植物保护学院, 福州 350002 wuzujian@126.com 
关雄 福建农林大学植物保护学院, 福州 350002 guanxfafu@126.com 
中文摘要:为监测小麦茎基腐病病原菌的变化,2019-2020年自河南省安阳、濮阳和鹤壁等17个市129个采样点采集小麦茎基腐病病株样品并分离病原菌,对所有分离物进行形态学鉴定,利用特异性引物、ITS和EF-1α基因序列进行分子生物学鉴定,并于室内对优势病原菌菌株进行致病力测定。结果表明,共分离得到892株镰孢菌Fusarium分离物,所得分离物中假禾谷镰孢F.pseudograminearum共851株,所占比例为95.41%,为优势种;禾谷镰孢F.graminearum共16株,所占比例为1.79%。除信阳市外,其他16个市优势病原菌均为假禾谷镰孢。不同假禾谷镰孢菌株存在明显的致病力分化,但多数为强致病力菌株。
中文关键词:小麦茎基腐病  病原菌  优势种  假禾谷镰孢  致病力分化
 
Identification and virulence determination of pathogens of wheat crown rot in Henan Province in 2019—2020
Author NameAffiliationE-mail
Gao Fei College Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
National Key Laboratory of Wheat and Maize Crop Science, College Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China 
 
Xie Yuan College Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
National Key Laboratory of Wheat and Maize Crop Science, College Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China 
 
Pan Xin National Key Laboratory of Wheat and Maize Crop Science, College Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China  
Li Honglian National Key Laboratory of Wheat and Maize Crop Science, College Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China  
Zhang Xiaoting National Key Laboratory of Wheat and Maize Crop Science, College Plant Protection, Henan Agricultural University, Zhengzhou 450002, Henan Province, China  
Wu Zujian College Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China wuzujian@126.com 
Guan Xiong College Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China guanxfafu@126.com 
Abstract:To monitor the changes of pathogen causing wheat crown rot in Henan Province, rotten wheat crown samples were collected and isolated for pathogenic fungi from 129 sites in 17 cities including Anyang, Puyang and Hebi etc. in Henan Province, in 2019-2020. Morphological and molecular biological identification including PCR amplification with specific primers and partial sequence analysis of ITS and EF-1α genes were done for all isolates. Then pathogenicity of the dominant pathogenic fungi was determined indoor. Results showed that among the total of 892 Fusarium isolates obtained, 851 isolates were identified as F. pseudograminearum, the dominant species, accounting for 95.41%, while 16 isolates were identified as F. graminearum, accounting for 1.79%. F. pseudograminearum dominated in all other sampling sites in Henan Province except for Xinyang. Although obvious virulence variation was detected among isolates of F. pseudograminearum, majority of them were highly virulent on wheat.
keywords:wheat crown rot  pathogen  dominant species  Fusarium pseudograminearum  pathogenicity differentiation
查看全文  查看/发表评论  下载PDF阅读器
您是本站第  8835820 版权所有:植物保护学报    京ICP备05006550号-2  
主管单位:中国科协 主办单位:中国植物保护学会、中国农业大学 地址:北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部
电话:010-62732528 电子邮件:zbxb@cau.edu.cn
技术支持:北京勤云科技发展有限公司