| 海南省田间稻瘟病菌中AvrPiz-t基因位点变异 |
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| 引用本文：张敏,肖倩,阮孙美,张攀,胡吉会,陈亚莉,罗琼.海南省田间稻瘟病菌中AvrPiz-t基因位点变异.植物保护学报,2023,50(2):325-332 |
| DOI：10.13802/j.cnki.zwbhxb.2023.2022061 |
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| 作者 | 单位 | E-mail | | 张敏 | 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201 | | | 肖倩 | 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201 | | | 阮孙美 | 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201 | | | 张攀 | 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201 | | | 胡吉会 | 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201 | | | 陈亚莉 | 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201 | | | 罗琼 | 云南农业大学植物保护学院, 云南生物资源保护与利用国家重点实验室, 昆明 650201 | qiongbf@aliyun.com |
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| 中文摘要:为评估在水稻育种中被广泛利用的广谱抗稻瘟病基因Piz-t的有效性,对不同年份分离自海南省陵水黎族自治县和三亚市水稻的273株田间稻瘟病菌株中的AvrPiz-t位点变异及其与菌株致病性的相关性进行系统研究。结果表明,海南省田间菌株中无毒基因AvrPiz-t位点的变异频率为0~100.00%,陵水黎族自治县菌株中的变异频率远远高于三亚市菌株。在菌株中共鉴定到3种Avr-Piz-t位点变异类型,分别为基因位点完全缺失、DNA重复元件MGR583在基因位点启动子区-10 bp上游和编码区218 bp下游插入。所有AvrPiz-t位点变异的菌株对携带Piz-t抗病基因的单基因水稻系IRBL-11均表现出强的致病性。陵水黎族自治县菌株中AvrPiz-t位点的变异频率呈逐年增加趋势,2021年94株菌株中AvrPiz-t位点的变异频率为100.00%,其中51.06%的菌株变异是MGR583在启动子区-10 bp上游插入,表明DNA重复元件MGR583在AvrPiz-t位点插入是AvrPiz-t从无毒到有毒进化的重要机制之一。 |
| 中文关键词:稻瘟病菌 无毒基因 AvrPiz-t 毒性变异 MGR583元件 |
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| Variations of the AvrPiz-t gene locus in field Magnaporthe oryzae strains collected from Hainan Province |
| Author Name | Affiliation | E-mail | | Zhang Min | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Xiao Qian | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Ruan Sunmei | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Zhang Pan | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Hu Jihui | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Chen Yali | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | | | Luo Qiong | State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, College of Plant Protection, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China | qiongbf@aliyun.com |
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| Abstract:To evaluate the effectiveness of the broad-spectrum blast resistance gene Piz-t widely used in rice resistance breeding, the investigation of AvrPiz-t (the Magnaporthe oryzae avirulence gene) locus variation in 273 field strains collected from Lingshui and Sanya of Hainan Province were carried out, and the correlation between pathogenicity and AvrPiz-t locus variation of strains was analyzed. The results showed that the variation frequency of AvrPiz-t locus in Hainan field strains ranged from 0 to 100.00%, and the variation frequency in the isolates from Lingshui was much higher than that in the isolates from Sanya. Three variant types of AvrPiz-t locus were identified in 273 field strains, including whole-gene deletion, the insertion of the repeat DNA element MGR583 at 10 bp upstream or 218 bp downstream from the transcription start site (TSS). All the tested isolates with variant AvrPiz-t locus were virulent to the monogenic rice line IRBL-11 containing cognate R gene Piz-t. The variation frequency of AvrPiz-t locus among the Lingshui isolates displayed a rising trend year by year. It is noteworthy that the variation frequency of AvrPiz-t locus in the 94 isolates collected from Lingshui in 2021 was 100.00%, of which, 51.06% was caused by MGR583 insertion at 10 bp upstream from the TSS. The results indicated that the insertion of the DNAelement MGR583 at AvrPiz-t locus was one of the important factor driving evolution of the blast fungal isolates in Hainan field from avirulence to virulence to the blast resistance gene Piz-t. |
| keywords:Magnaporthe oryzae avirulence gene AvrPiz-t virulence variation MGR583 element |
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