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小RNA生物合成途径主要元件调控稻瘟病菌生长发育和致病性
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引用本文:张丽梅,项旭跃,齐敏,曹雪琦,王宗华,郑华坤.小RNA生物合成途径主要元件调控稻瘟病菌生长发育和致病性.植物保护学报,2023,50(2):333-342
DOI:10.13802/j.cnki.zwbhxb.2023.2021105
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作者单位E-mail
张丽梅 福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 福州 350002  
项旭跃 福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 福州 350002  
齐敏 福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 福州 350002  
曹雪琦 福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 福州 350002  
王宗华 福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 福州 350002
闽江学院海洋研究院, 福州 350108 
 
郑华坤 福建农林大学植物保护学院, 闽台作物有害生物生态防控国家重点实验室, 福州 350002 huakunzheng@163.com 
中文摘要:为探究小RNA生物合成途径主要蛋白在稻瘟病菌Magnaporthe oryzae生长发育、胁迫响应和致病过程中的作用,在野生型菌株Guy11背景下,分别构建其单基因敲除突变体ΔMoago3、ΔModcl2、ΔMordrp2和双基因敲除突变体ΔMoago3ΔModcl2、ΔMoago3ΔMordrp2、ΔModcl2ΔMordrp2,并测定不同突变体的营养生长、产孢过程、对不同胁迫的响应、致病性及侵染菌丝类型。结果显示,仅双基因敲除突变体ΔMoago3ΔModcl2和ΔMoago3ΔMordrp2的菌落直径较野生型菌株Guy11显著减少;突变体ΔModcl2和ΔModcl2ΔMordrp2的产孢量分别显著下降至野生型菌株Guy11和突变体ΔMordrp2的66.67%;十二烷基硫酸钠(sodium dodecylsulfate,SDS)、NaCl、山梨醇和H2O2胁迫对各突变体的抑制率影响不显著,但在刚果红胁迫下,突变体ΔMoago3、ΔMordrp2和ΔMoago3ΔModcl2的抑制率均显著高于野生型菌株Guy11的抑制率;与野生型菌株Guy11相比,突变体ΔMoago3、ΔModcl2、ΔMordrp2和ΔModcl2ΔMordrp2的致病性略微减弱,突变体ΔMoago3ΔModcl2和ΔMoago3ΔMordrp2的致病性则明显减弱,而且ΔMoago3、ΔModcl2、ΔMoago3ΔModcl2、ΔMoago3ΔMordrp2和ΔModcl2ΔMordrp2中类型3和类型4侵染菌丝的比例也相应减少。表明小RNA生物合成途径主要元件在稻瘟病菌生长发育、胁迫响应和致病过程中均发挥着重要的调控作用。
中文关键词:稻瘟病菌  小RNA  生长发育  致病性  胁迫响应
 
Major components of sRNA biogenesis pathway are required for development and pathogenicity in rice blast fungus
Author NameAffiliationE-mail
Zhang Limei State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China  
Xiang Xuyue State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China  
Qi Min State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China  
Cao Xueqi State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China  
Wang Zonghua State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China
Institute of Oceanography, Minjiang University, Fuzhou 350108, Fujian Province, China 
 
Zheng Huakun State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, Fujian Province, China huakunzheng@163.com 
Abstract:To investigate the role of the major components of small RNA (sRNA) biosynthesis pathway in the development, stress response and pathogenicity of the rice blast fungus Magnaporthe oryzae, the single-gene knockout mutants ΔMoago3, ΔModcl2, ΔMordrp2 and double-gene knockout mutants ΔMoago3ΔModcl2, ΔMoago3ΔMordrp2 and ΔModcl2ΔMordrp2 were constructed in the wild type (Guy11) background, and the vegetative growth, conidiation, stress response, pathogenicity and invasive growth of these mutants were investigated. The results showed that, the fungal growth was only significantly reduced in the two double-gene deletion mutants, ΔMoago3ΔModcl2 and ΔMoago3ΔMordrp2, compared with the wild type strain Guy11; conidiation of ΔModcl2 and ΔModcl2ΔMordrp2 was significantly reduced to 66.67% of that of wild type strain Guy11 and ΔMordrp2; compared with the wild type strain Guy11, the inhibition rates of all the six mutants were not significantly altered upon the treatment of sodium dodecylsulfate (SDS), NaCl, sorbitol and H2O2, while under the treatment of Congo red, the inhibition rates of ΔMoago3, ΔMordrp2 and ΔMoago3ΔModcl2 were significantly increased; moreover, pathogenicity was slightly compromised in ΔMoago3, ΔModcl2, ΔMordrp2 and ΔModcl2ΔMordrp2, and was severely compromised in ΔMoago3ΔModcl2 and ΔMoago3ΔMordrp2; consistently, the percentages of type 3 and type 4 invasive hyphae overtly decreased in ΔMoago3, ΔModcl2, ΔMoago3ΔModcl2, ΔMoago3ΔMordrp2 and ΔModcl2ΔMordrp2. These results suggested that the major components of sRNA biogenesis are involved in the regulation of asexual development, stress response and pathogenicity in rice blast fungus.
keywords:rice blast fungus  sRNA  development  pathogenicity  stress response
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