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基于高通量测序检测甜菜生尾孢中真菌病毒多样性

引用本文：李盈曦,周梦轲,杨一舟,鲍方维,韩成贵,王颖.基于高通量测序检测甜菜生尾孢中真菌病毒多样性.植物保护学报,2023,50(2):370-379

DOI：10.13802/j.cnki.zwbhxb.2023.2021085

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作者	单位	E-mail
李盈曦	中国农业大学植物保护学院, 农业农村部病虫害监测与绿色管理重点实验室, 北京 100193
周梦轲	中国农业大学植物保护学院, 农业农村部病虫害监测与绿色管理重点实验室, 北京 100193
杨一舟	中国农业大学生物学院, 北京 100193
鲍方维	中国农业大学植物保护学院, 农业农村部病虫害监测与绿色管理重点实验室, 北京 100193
韩成贵	中国农业大学植物保护学院, 农业农村部病虫害监测与绿色管理重点实验室, 北京 100193
王颖	中国农业大学植物保护学院, 农业农村部病虫害监测与绿色管理重点实验室, 北京 100193	yingwang@cau.edu.cn

中文摘要:为明确甜菜生尾孢Cercospora beticola中真菌病毒的多样性,2020年自新疆维吾尔族自治区、内蒙古自治区、黑龙江省和北京市采集感染褐斑病甜菜病叶,鉴定获得78株甜菜生尾孢菌株,运用高通量的宏转录组测序技术检测甜菜生尾孢所携带的真菌病毒种类;将分离到的甜菜生尾孢按照菌落生长表型分为4组,进行宏转录组测序,将得到的序列经过拼接、聚类和基因功能注释,筛选出真菌病毒相关同源序列。序列分析结果显示,4组样品共比对到6个真菌病毒科,包括双分体病毒科Partitiviridae、双核糖核酸病毒科Birnaviridae、低毒病毒科Hypoviridae、裸露RNA病毒科Narnaviridae、泛欧尔密病毒科Botourmiaviridae和转座病毒科Metaviridae,以及未分科的负义单链RNA病毒。研究结果丰富了甜菜生尾孢真菌病毒资源库。

中文关键词:甜菜生尾孢高通量测序真菌病毒低毒病毒双分体病毒

The discovery of mycoviruses associated with the plant pathogenic fungus Cercospora beticola with high-throughput sequencing

Author Name	Affiliation	E-mail
Li Yingxi	Key Laboratory of Pest Monitoring and Green Management of Ministry of Agriculture and Rural Affairs, College of Plant Protection, China Agricultural University, Beijing 100193
Zhou Mengke	Key Laboratory of Pest Monitoring and Green Management of Ministry of Agriculture and Rural Affairs, College of Plant Protection, China Agricultural University, Beijing 100193
Yang Yizhou	College of Biological Sciences, China Agricultural University, Beijing 100193, China
Bao Fangwei	Key Laboratory of Pest Monitoring and Green Management of Ministry of Agriculture and Rural Affairs, College of Plant Protection, China Agricultural University, Beijing 100193
Han Chenggui	Key Laboratory of Pest Monitoring and Green Management of Ministry of Agriculture and Rural Affairs, College of Plant Protection, China Agricultural University, Beijing 100193
Wang Ying	Key Laboratory of Pest Monitoring and Green Management of Ministry of Agriculture and Rural Affairs, College of Plant Protection, China Agricultural University, Beijing 100193	yingwang@cau.edu.cn

Abstract:To explore the diversity of mycoviruses associated with the plant pathogenic fungus Cercospora beticola, 78 single-spore isolates of C. beticola collected from several fields in Xinjiang Uygur Autonomous Region, Inner Mongolia Autonomous Region, Heilongjiang Province and Beijing City in 2020 were used for metatranscriptome sequencing analysis. Isolates were divided into four groups based on different colony phenotype and sequenced with high-throughput sequencing. Raw data were transcribed, clustered and functionally annotated. A total number of six virus families and a few unclassified (-)ssRNA viruses were identified according to the bioinformatics analysis. The identified fungi-associated viruses belong to Partitiviridae, Birnaviridae, Hypoviridae, Narnaviridae, Botourmiaviridae and Metaviridae. This study enriched the mycoviruses resource of C. beticola.

keywords:Cercospora beticola high-throughput sequencing mycovirus Hypovirus Partitivirus

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