| MfMel1基因参与调控桃褐腐病菌对桃果实的致病力 |
| 点此下载全文 |
| 引用本文：谷文倩,阴伟晓,罗朝喜.MfMel1基因参与调控桃褐腐病菌对桃果实的致病力.植物保护学报,2023,50(2):380-390 |
| DOI：10.13802/j.cnki.zwbhxb.2023.2021076 |
| 摘要点击次数: |
| 全文下载次数: |
|
|
| 中文摘要:为明确MfMel1基因在桃褐腐病菌Monilinia fructicola中的功能,利用生物信息学软件对该基因的结构、所编码蛋白的保守结构域以及进化关系进行分析;通过遗传转化方法对该基因进行敲除,并进行表型分析。结果显示,敲除转化子△MfMel1菌株的菌丝生长速率、孢子萌发率与野生型菌株Bmpc7均无显著差异,但产孢量显著降低。△MfMel1菌株在0.01% SDS、600 μg/mL刚果红、150 g/L甘油、200 g/L蔗糖、6 mmol/L H2O2、4% NaCl和1.2 mmol/L山梨醇培养基上的生长速率、菌丝形态均无显著变化,但对200 g/L葡萄糖的渗透胁迫敏感性增强。敲除转化子△MfMel1菌株的α-半乳糖苷酶活性明显降低,对桃果实的致病力显著下降。表明MfMel1基因在桃褐腐病菌中可能通过参与调控α-半乳糖苷酶酶活,从而参与病原菌的致病。 |
| 中文关键词:桃褐腐病菌 MfMel1基因 糖苷水解酶 侵染机制 α-半乳糖苷酶 |
| |
| MfMel1 gene participates in the pathogenicity of pathogen fungus Monilinia fructicola on peach fruits |
| Author Name | Affiliation | E-mail | | Gu Wenqian | National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China | | | Yin Weixiao | Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China | | | Luo Chaoxi | National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China
Hubei Key Laboratory of Plant Pathology, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China | cxluo@mail.hzau.edu.cn |
|
|
| Abstract:In order to illustrate the functions of MfMel1 in pathogen fungus Monilinia fructicola, the gene structure, conserved domain and evolutionary relationships of the predicted protein were analyzed using bioinformatic software. The gene was knocked out through the genetic transformation, and the phenotypes of the mutants were investigated. No significant difference was observed for the mycelial growth and spore germination between knockout transformants △MfMel1 and wild type strain Bmpc7. However, the sporulation of △MfMel1 strains decreased significantly compared to Bmpc7. △MfMel1 strains showed increased sensitivity to 200 g/L glucose, but not to 0.01% SDS, 600 μg/mL congo red, 150g/Lglycerol,200g/Lsucrose,6mmol/LH2O2,4%NaCl,or 1.2mmol/Lsorbitol.△MfMel1 strains exhibited significantly decreased virulence on peach fruit and reduced activity of α-galactosidase. The results indicated that MfMel1 was involved in regulating the α-galactosidase activity, thus playing an important role during the infection of M. fructicola on peach fruit. |
| keywords:peach brown rot MfMel1 gene glycoside reductase infection mechanism α-galactosidase |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 14347303 版权所有：植物保护学报 京ICP备05006550号-2 主管单位：中国科协 主办单位：中国植物保护学会、中国农业大学 地址：北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话：010-62732528 电子邮件：zbxb@cau.edu.cn 技术支持： 北京勤云科技发展有限公司 |
