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褐飞虱化学感受蛋白基因克隆与信号肽活性鉴定
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引用本文:刘傲,郭倩汝,史韵竹,王满囷.褐飞虱化学感受蛋白基因克隆与信号肽活性鉴定.植物保护学报,2023,50(2):430-437
DOI:10.13802/j.cnki.zwbhxb.2023.2021107
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作者单位E-mail
刘傲 华中农业大学植物科学技术学院, 武汉 430070  
郭倩汝 华中农业大学植物科学技术学院, 武汉 430070  
史韵竹 华中农业大学植物科学技术学院, 武汉 430070  
王满囷 华中农业大学植物科学技术学院, 武汉 430070 mqwang@mail.hzau.edu.cn 
中文摘要:为科学利用化学感受蛋白(chemosensory protein,CSP)绿色防控褐飞虱Nilaparvata lugens,采用同源序列比对和基因克隆对褐飞虱CSP基因进行鉴定,利用基因组分析其CSP基因簇,通过酵母信号肽分泌验证系统验证褐飞虱CSP信号肽的活性。结果显示,共克隆了15个褐飞虱CSP基因,其中有4个CSP基因为新鉴定到的;共发现了2个CSP基因簇,均位于褐飞虱2号染色体上;14个CSP的N末端序列有较强的信号肽分泌活性,其中11个活性序列与SignalP-4.1预测的序列一致,另外3个CSP的活性序列比SignalP-4.1预测的序列长。
中文关键词:褐飞虱  化学感受蛋白  基因克隆  基因簇  信号肽活性
 
Cloning of chemosensory protein genes and identification of signal peptide activity in brown planthopper Nilaparvata lugens
Author NameAffiliationE-mail
Liu Ao College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China  
Guo Qianru College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China  
Shi Yunzhu College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China  
Wang Manqun College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, Hubei Province, China mqwang@mail.hzau.edu.cn 
Abstract:To develop green prevention and control methods against brown planthopper Nilaparvata lugens based on chemosensory proteins (CSPs), the CSP genes were identified with homologous sequence alignment and gene cloning. The CSP gene clusters were analyzed with its genome, and the signal peptide activities of CSPs were verified with yeast signal peptide secretion verification system. The results showed that a total of 15 CSP genes were cloned from Nilaparvata lugens, of which four were newly identified. Two CSP gene clusters were identified, and both were located on chromosome 2. The Nterminal sequences of 14 CSPs showed strong signal peptide secretion activity, 11 of which were consistent with SignalP-4.1 prediction sequences, and the other three were longer than prediction.
keywords:Nilaparvata lugens  chemosensoryprotein  genecloning  genecluster  signalpeptideactivity
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