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黄翅绢丝野螟气味受体基因GcaeOR10的鉴定及组织表达分析

引用本文：张绍华,孟倩倩,朱恩杭,查学宗,颜日辉,刘爱勤,吴刚,王政.黄翅绢丝野螟气味受体基因GcaeOR10的鉴定及组织表达分析.植物保护学报,2023,50(2):438-445

DOI：10.13802/j.cnki.zwbhxb.2022.2021184

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作者	单位	E-mail
张绍华	海南大学植物保护学院, 热带农林生物灾害绿色防控教育部重点实验室, 海口 570228 中国热带农业科学院香料饮料研究所, 海南省热带香辛饮料作物遗传改良与品质调控重点实验室, 万宁 571533
孟倩倩	中国热带农业科学院香料饮料研究所, 海南省热带香辛饮料作物遗传改良与品质调控重点实验室, 万宁 571533	mengzouzou@163.com
朱恩杭	海南大学植物保护学院, 热带农林生物灾害绿色防控教育部重点实验室, 海口 570228
查学宗	海南大学植物保护学院, 热带农林生物灾害绿色防控教育部重点实验室, 海口 570228
颜日辉	海南大学植物保护学院, 热带农林生物灾害绿色防控教育部重点实验室, 海口 570228
刘爱勤	海南大学植物保护学院, 热带农林生物灾害绿色防控教育部重点实验室, 海口 570228 中国热带农业科学院香料饮料研究所, 海南省热带香辛饮料作物遗传改良与品质调控重点实验室, 万宁 571533
吴刚	中国热带农业科学院香料饮料研究所, 海南省热带香辛饮料作物遗传改良与品质调控重点实验室, 万宁 571533
王政	海南大学植物保护学院, 热带农林生物灾害绿色防控教育部重点实验室, 海口 570228 中国热带农业科学院香料饮料研究所, 海南省热带香辛饮料作物遗传改良与品质调控重点实验室, 万宁 571533 重庆市疾病预防控制中心消毒与媒介生物控制所, 高致病性病原微生物重庆市重点实验室, 重庆 400042	sallywz618@163.com

中文摘要:为明确黄翅绢丝野螟Glyphodes caesalis气味受体基因GcaeOR10(GenBank登录号MW701397)的生物信息学特征及组织表达谱,使用生物信息学软件对其进行序列分析,并通过实时荧光定量PCR (real-time fluorescence quantitative PCR,qPCR)技术检测其在黄翅绢丝野螟成虫不同组织中的表达量。结果显示,黄翅绢丝野螟GcaeOR10基因开放阅读框(open reading frame,ORF)全长为1 200 bp,编码399个氨基酸残基,预测其编码的蛋白质分子量为45.67 kD,等电点为8.65,无信号肽,具有昆虫气味受体家族7个跨膜蛋白(7 transmembrane proteins,7tm-6)超家族的保守结构域,存在26个潜在的磷酸化位点,含有7个跨膜结构域,且N端位于细胞膜内,C端位于细胞膜外。黄翅绢丝野螟GcaeOR10与桑螟Glyphodes pyloalis的GpylOR17亲缘关系最近,其次与松蛀螟Conogethes pinicolalis的CpinOR39、CpinOR36和桃蛀螟Conogethes punctiferalis的CpunOR36亲缘关系较近。黄翅绢丝野螟GcaeOR10基因在雄成虫触角中的表达量最高,在腹部的表达量次之。表明黄翅绢丝野螟GcaeOR10是典型的气味受体,可能与该虫识别性信息素有关,参与其寻找配偶及交配等行为过程。

中文关键词:黄翅绢丝野螟气味受体生物信息学分析组织表达谱

Identification and tissue expression profiling of the odorant receptor gene GcaeOR10 in jack fruit borer Glyphodes caesalis (Lepidoptera: Pyralidae)

Author Name	Affiliation	E-mail
Zhang Shaohua	Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Research Institute of Spice and Beverage, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, Hainan Province, China
Meng Qianqian	Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Research Institute of Spice and Beverage, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, Hainan Province, China	mengzouzou@163.com
Zhu Enhang	Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China
Zha Xuezong	Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China
Yan Rihui	Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China
Liu Aiqin	Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Research Institute of Spice and Beverage, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, Hainan Province, China
Wu Gang	Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Research Institute of Spice and Beverage, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, Hainan Province, China
Wang Zheng	Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education, College of Plant Protection, Hainan University, Haikou 570228, Hainan Province, China Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Research Institute of Spice and Beverage, Chinese Academy of Tropical Agricultural Sciences, Wanning 571533, Hainan Province, China Chongqing Municipal Key Laboratory for High Pathogenic Microbes, Department of Disinfection and Vector Control, Chongqing Center for Disease Control and Prevention, Chongqing 400042, China	sallywz618@163.com

Abstract:To clarify the bioinformatics characteristics and tissue expression profile of the odorant receptor gene GcaeOR10 in Glyphodes caesalis (GenBank accession no. MW701397), bioinformatics software was used for sequence analysis, and its expression in different tissues of adults was detected by real-time fluorescence quantitative PCR (qPCR). The results showed that the open reading frame (ORF) of GcaeOR10 gene was 1 200 bp in length, encoding 399 amino acid residues. The predicted molecular weight was 45.67 kD and the isoelectric point was 8.65. However, the amino acid sequence of GcaeOR10 had no signal peptide but contained a conserved domain belonging to odorant receptor seven transmembrane proteins (7tm-6) superfamily and 26 potential phosphorylation sites. Besides, it also contained seven transmembrane structures. The N-terminus was intramembrane and the C-terminus was extramembrane. GcaeOR10 had the closest genetic relationship with GpylOR17 of G. pyloalis, followed by CpinOR39 and CpinOR36 of Conogethes pinicolalis and CpunOR36 of Conogethes punctiferalis. The qPCR results revealed that the expression of GcaeOR10 was the highest in the antennae of male adults, followed by in the abdomen. These results indicated that GcaeOR10 was a typical odorant receptor, which might be related to the mechanism of sex pheromone recognition and participate in its behavior processes such as finding a spouse and mating.

keywords:Glyphodes caesalis odorant receptor bioinformatics analysis tissue expression profiling

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