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FKBP46对飞蝗卵滞育具有促进作用

引用本文：张道刚,陈俊,崔栋楠,李霜,潘凡,朱凯辉,涂雄兵,张泽华.FKBP46对飞蝗卵滞育具有促进作用.植物保护学报,2023,50(2):485-492

DOI：10.13802/j.cnki.zwbhxb.2023.2021161

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作者	单位	E-mail
张道刚	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
陈俊	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
崔栋楠	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
李霜	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
潘凡	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
朱凯辉	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193
涂雄兵	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193	xbtu@ippcaas.cn
张泽华	中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193	zhangzehua@caas.cn

中文摘要:为验证FK506结合蛋白46(FK506 binding protein 46,FKBP46)在调节飞蝗Locusta migrato-ria滞育中的作用,首先从飞蝗中克隆FKBP46,然后合成双链RNA,将其注入到飞蝗雌成虫体内进行干扰,观察干扰后钙调磷酸酶(calcineurin,CaN)的活性和卵滞育率的变化。结果显示,短日照条件下,RNA干扰FKBP46后飞蝗卵滞育率显著降低,由97.44%降至78.59%;在长日照条件下,RNA干扰FKBP46后,所有飞蝗卵均成功孵化,与对照组无显著差异;FKBP46基因沉默后,飞蝗后足中CaN活性分别从0.24 U/g上调至0.29 U/g,脂肪体中CaN活性从0.21 U/g上调至0.30 U/g。表明在短日照条件下FKBP46负调控CaN影响飞蝗滞育,FKBP46的沉默效应表明该调控基因可能是基于RNA干扰的飞蝗滞育控制的理想靶点。

中文关键词:飞蝗滞育 FKBP 钙调磷酸酶活性 RNA干扰

FKBP46 plays a role in promoting the egg diapause of the migratory locust, Locusta migratoria

Author Name	Affiliation	E-mail
Zhang Daogang	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Chen Jun	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Cui Dongnan	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Li Shuang	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Pan Fan	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Zhu Kaihui	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Tu Xiongbing	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China	xbtu@ippcaas.cn
Zhang Zehua	State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China	zhangzehua@caas.cn

Abstract:The aim of the present study was to verify the function of FK506 binding protein 46 (FKBP46) in regulating the diapause of the migratory locust, Locusta migratoria. FKBP46 was cloned from L. migratoria and the double-stranded RNA of FKBP46 was synthesized, which was then injected into L. migratoria female adults to observe the changes in calcineurin (CaN) activity and the rate of egg diapause. The results showed that RNA interference of FKBP46 significantly decreased egg diapause rates from 97.44% to 78.59% under short photoperiods. Under long photoperiod, all L. migratoria eggs hatched after FKBP46 RNA interference, with no difference from the control group. Meanwhile, CaN activity was up-regulated from 0.24 U/g to 0.29 U/g in the hind leg and from 0.21 U/g to 0.30 U/g in the fat body, respectively, after FKBP46 gene silencing. These results suggested that FKBP46 negatively regulated CaN to affect L. migratoria diapause under short photoperiod. This regulatory gene is probably an ideal target for RNAi-based diapause control of the migratory locust.

keywords:Locusta migratoria diapause FKBP calcineurin activity RNA interference

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