FKBP46对飞蝗卵滞育具有促进作用 |
点此下载全文 |
引用本文:张道刚,陈俊,崔栋楠,李霜,潘凡,朱凯辉,涂雄兵,张泽华.FKBP46对飞蝗卵滞育具有促进作用.植物保护学报,2023,50(2):485-492 |
DOI:10.13802/j.cnki.zwbhxb.2023.2021161 |
摘要点击次数: |
全文下载次数: |
作者 | 单位 | E-mail | 张道刚 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | 陈俊 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | 崔栋楠 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | 李霜 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | 潘凡 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | 朱凯辉 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | | 涂雄兵 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | xbtu@ippcaas.cn | 张泽华 | 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193 | zhangzehua@caas.cn |
|
|
中文摘要:为验证FK506结合蛋白46(FK506 binding protein 46,FKBP46)在调节飞蝗Locusta migrato-ria滞育中的作用,首先从飞蝗中克隆FKBP46,然后合成双链RNA,将其注入到飞蝗雌成虫体内进行干扰,观察干扰后钙调磷酸酶(calcineurin,CaN)的活性和卵滞育率的变化。结果显示,短日照条件下,RNA干扰FKBP46后飞蝗卵滞育率显著降低,由97.44%降至78.59%;在长日照条件下,RNA干扰FKBP46后,所有飞蝗卵均成功孵化,与对照组无显著差异;FKBP46基因沉默后,飞蝗后足中CaN活性分别从0.24 U/g上调至0.29 U/g,脂肪体中CaN活性从0.21 U/g上调至0.30 U/g。表明在短日照条件下FKBP46负调控CaN影响飞蝗滞育,FKBP46的沉默效应表明该调控基因可能是基于RNA干扰的飞蝗滞育控制的理想靶点。 |
中文关键词:飞蝗 滞育 FKBP 钙调磷酸酶活性 RNA干扰 |
|
FKBP46 plays a role in promoting the egg diapause of the migratory locust, Locusta migratoria |
Author Name | Affiliation | E-mail | Zhang Daogang | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | Chen Jun | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | Cui Dongnan | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | Li Shuang | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | Pan Fan | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | Zhu Kaihui | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | | Tu Xiongbing | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | xbtu@ippcaas.cn | Zhang Zehua | State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China | zhangzehua@caas.cn |
|
|
Abstract:The aim of the present study was to verify the function of FK506 binding protein 46 (FKBP46) in regulating the diapause of the migratory locust, Locusta migratoria. FKBP46 was cloned from L. migratoria and the double-stranded RNA of FKBP46 was synthesized, which was then injected into L. migratoria female adults to observe the changes in calcineurin (CaN) activity and the rate of egg diapause. The results showed that RNA interference of FKBP46 significantly decreased egg diapause rates from 97.44% to 78.59% under short photoperiods. Under long photoperiod, all L. migratoria eggs hatched after FKBP46 RNA interference, with no difference from the control group. Meanwhile, CaN activity was up-regulated from 0.24 U/g to 0.29 U/g in the hind leg and from 0.21 U/g to 0.30 U/g in the fat body, respectively, after FKBP46 gene silencing. These results suggested that FKBP46 negatively regulated CaN to affect L. migratoria diapause under short photoperiod. This regulatory gene is probably an ideal target for RNAi-based diapause control of the migratory locust. |
keywords:Locusta migratoria diapause FKBP calcineurin activity RNA interference |
查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
|
您是本站第 7511533
版权所有:植物保护学报 京ICP备05006550号-2
主管单位:中国科协 主办单位:中国植物保护学会、中国农业大学 地址:北京市圆明园西路2号 中国农业大学植物保护学院 植物保护学报编辑部 电话:010-62732528 电子邮件:zbxb@cau.edu.cn
技术支持: 北京勤云科技发展有限公司
etiller();
|