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抑霉唑、咪鲜胺与指状青霉CYP51的结合模式及交互抗性机制分析

引用本文：李康,肖春,严飘,陶丽红,李佳俊,吴文伟,叶敏,王凯博.抑霉唑、咪鲜胺与指状青霉CYP51的结合模式及交互抗性机制分析.植物保护学报,2023,50(2):545-556

DOI：10.13802/j.cnki.zwbhxb.2023.2021104

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作者	单位	E-mail
李康	云南农业大学, 云南生物资源保护与利用国家重点实验室, 昆明 650201
肖春	云南农业大学, 云南生物资源保护与利用国家重点实验室, 昆明 650201
严飘	云南农业大学, 云南生物资源保护与利用国家重点实验室, 昆明 650201
陶丽红	云南农业大学, 云南生物资源保护与利用国家重点实验室, 昆明 650201
李佳俊	云南农业大学, 云南生物资源保护与利用国家重点实验室, 昆明 650201
吴文伟	云南省农业科学院农业环境资源研究所, 昆明 650201
叶敏	云南农业大学, 云南生物资源保护与利用国家重点实验室, 昆明 650201	yeminpc@163.com
王凯博	云南省农业科学院茶叶研究所, 昆明 650201	405052973@qq.com

中文摘要:为解析指状青霉Penicillium digitatum对抑霉唑和咪鲜胺的抗性机制和交互抗性机制,基于指状青霉甾醇14α-去甲基化酶(sterol 14α-demethylases,CYP51) CYP51A和CYP51B的氨基酸序列,采用同源建模的方法构建其三维结构,根据已报道的可能与抑霉唑抗性相关的突变位点CYP51A-Y308H及与咪鲜胺抗性相关的突变位点CYP51B-Y136H、CYP51B-Q309H和CYP51BF506I构建突变模型,采用分子对接法分析其突变前后与抑霉唑、咪鲜胺的结合模式,并进行氨基酸序列保守性分析。结果显示,抑霉唑和咪鲜胺与指状青霉CYP51的结合无特异性,两者均能与其CYP51A和CYP51B结合。指状青霉的CYP51A-Y308氨基酸残基及CYP51A-Y308H突变体氨基酸残基均不能与抑霉唑和咪鲜胺形成相互作用力,说明该突变与指状青霉对抑霉唑和咪鲜胺的抗性无关。指状青霉的CYP51B-Y136H突变导致其与咪鲜胺和抑霉唑的亲和力下降;指状青霉的CYP51B-Y136氨基酸位于CYP51B蛋白的保守区域,其产生的与咪鲜胺和抑霉唑抗性相关的随机突变在药剂选择压力下的分布频率可能逐渐上升。表明指状青霉CYP51B-Y136氨基酸突变可能是其对咪鲜胺及抑霉唑产生抗性及交互抗性的原因之一。

中文关键词:指状青霉甾醇14α-去甲基化酶（CYP51）抑霉唑咪鲜胺交互抗性

Analysis of cross-resistance to imazalil and prochloraz and their binding modes with CYP51 in pathogenic fungus Penicillium digitatum

Author Name	Affiliation	E-mail
Li Kang	State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China
Xiao Chun	State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China
Yan Piao	State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China
Tao Lihong	State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China
Li Jiajun	State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China
Wu Wenwei	Institute of Agricultural Environment and Resource, Yunnan Academy of Agricultural Sciences, Kunming 650201, Yunnan Province, China
Ye Min	State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, Yunnan Province, China	yeminpc@163.com
Wang Kaibo	Institute of Tea, Yunnan Academy of Agricultural Sciences, Kunming 650201, Yunnan Province, China	405052973@qq.com

Abstract:In order to analyze the mechanisms of resistance and cross-resistance to imazalil and prochloraz in pathogenic fungus Penicillium digitatum, the three-dimensional structure of sterol 14α-demethylases (CYP51) of P. digitatum were constructed by homology modeling according to the amino acid sequence of CYP51A and CYP51B, respectively. Then, the protein models of mutants CYP51A/CYP51B were constructed according to the reported mutation site CYP51A-Y308H which might relate to the resistance of P. digitatum to imazalil and CYP51B-Y136H/Q309H/F506I which might relate to the resistance of P. digitatum to prochloraz. The binding modes of the wild-type and mutants CYP51A/CYP51B with imazalil and prochloraz were analyzed by molecular docking, respectively. Besides, the conservation of amino acid sequence of CYP51A and CYP51B was also analyzed. The results showed that imazalil and prochloraz were non-specific, targeting both CYP51A and CYP51B of P. digitatum. Neither the Y308 residue of CYP51A-Y308 nor the Y308 residue of CYP51A-Y308H mutations in P. digitatum could obviously interact with imazalil or prochloraz, suggesting that the CYP51A-Y308H mutation was irrelevant to the resistance to imazalil and prochloraz in P. digitatum. The CYP51B-Y136H mutation could reduce the binding affinity to imazalil and prochloraz in P. digitatum, which was one of the reasons that caused the resistance and cross-resistance to imazalil and prochloraz. The Y136 residue was located in the conserved region of CYP51B in P. digitatum, and the frequency of random mutations related to the resistance to prochloraz and imazalil might gradually increase under the fungicides selective pressure. The findings from this study evidenced that the CYP51B-Y136H mutation in P. digitatum should be paid more attention in the future.

keywords:Penicillium digitatum sterol 14α-demethylases (CYP51) imazalil prochloraz cross-resistance

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